Strain identifier

BacDive ID: 6493

Type strain: No

Species: Lactiplantibacillus plantarum subsp. plantarum

Strain Designation: P1

Strain history: CIP <- 1971, F. Gasser, Inst. Pasteur, Paris, France: strain P1

NCBI tax ID(s): 337330 (subspecies)

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General

@ref: 1148

BacDive-ID: 6493

DSM-Number: 2601

keywords: genome sequence, Bacteria, facultative anaerobe, mesophilic, human pathogen

description: Lactiplantibacillus plantarum subsp. plantarum P1 is a facultative anaerobe, mesophilic human pathogen that was isolated from pickled cabbage.

NCBI tax id

  • NCBI tax id: 337330
  • Matching level: subspecies

strain history

@refhistory
1148<- NCIB <- NCTC <- M. Stephenson
398141971, F. Gasser, Inst. Pasteur, Paris, France: strain P1
67770ATCC 10241 <-- M. Stephenson.
121860CIP <- 1971, F. Gasser, Inst. Pasteur, Paris, France: strain P1

doi: 10.13145/bacdive6493.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactiplantibacillus
  • species: Lactiplantibacillus plantarum subsp. plantarum
  • full scientific name: Lactiplantibacillus plantarum subsp. plantarum (Orla-Jensen 1919) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus plantarum subsp. plantarum

@ref: 1148

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactiplantibacillus

species: Lactiplantibacillus plantarum subsp. plantarum

full scientific name: Lactiplantibacillus plantarum subsp. plantarum (Orla-Jensen 1919) Zheng et al. 2020

strain designation: P1

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no97.346
69480100positive
121860nopositiverod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1148MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
39814MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
121860CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
1148positivegrowth30mesophilic
39814positivegrowth37mesophilic
67770positivegrowth30mesophilic
121860positivegrowth22-37
121860nogrowth10psychrophilic
121860nogrowth15psychrophilic
121860nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 121860
  • oxygen tolerance: facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.997

compound production

  • @ref: 1148
  • compound: acetylcholine

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371melezitose+builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371D-sorbitol+builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
121860nitrate-reduction17632
121860nitrite-reduction16301
121860nitrate+respiration17632

metabolite production

  • @ref: 67770
  • Chebi-ID: 15355
  • metabolite: acetylcholine
  • production: yes

metabolite tests

  • @ref: 121860
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
121860oxidase-
121860alcohol dehydrogenase-1.1.1.1
121860catalase-1.11.1.6
121860lysine decarboxylase-4.1.1.18
121860ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121860--++-+-+--++-+-+++--

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
1148----++----++++----+++-+++++++++++-++---++---------
121860----+----++++-+--+++/--+++++++++++-+----++---------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121860---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
1148pickled cabbage
67770Pickled cabbage
121860Food, SauerkrautUnited KingdomGBREurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Food
#Engineered#Food production#Vegetable (incl. Grains)
#Host#Plants#Herbaceous plants (Grass,Crops)
#Condition#Saline

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
1148yes, in single cases1Risk group (German classification)
1218601Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactiplantibacillus plantarum DSM 2601GCA_001888655contigncbi1590
66792Lactobacillus plantarum strain DSM 26011590.345wgspatric1590

GC content

  • @ref: 67770
  • GC-content: 45
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno94.426no
gram-positiveyes96.007no
anaerobicno89.437no
aerobicno97.227no
halophileyes85.208no
spore-formingno92.924no
thermophileno99.884yes
glucose-utilyes92.752no
flagellatedno97.032no
glucose-fermentyes89.132no

External links

@ref: 1148

culture collection no.: DSM 2601, ATCC 10241, NCDO 343, NCIB 7220, JCM 1551, BCRC 12251, CECT 749, CIP 71.39, KCTC 3104, LMG 9208, NCFB 343, NCIMB 7220, NCIMB 8184, NCTC 7220

straininfo link

  • @ref: 75966
  • straininfo: 265314

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Cultivation457268Serological studies on chemostat-grown cultures of Lactobacillus fermentum and Lactobacillus plantarum.Knox KW, Campbell LK, Broady KW, Wicken AJInfect Immun10.1128/iai.24.1.12-18.19791979Antibody Specificity, Antigens, Bacterial/*analysis, Antigens, Surface/analysis, Cell Wall/immunology, Culture Media, Lactobacillus/*classification/immunology, Polysaccharides, Bacterial/immunology, Ribitol, Serotyping, Teichoic Acids/immunologyPhylogeny
Phylogeny907345Isolation, extraction, and measurement of acetylcholine from Lactobacillus plantarum.Stanaszek PM, Snell JF, O'Neill JJAppl Environ Microbiol10.1128/aem.34.2.237-239.19771977Acetonitriles, Acetylcholine/analysis/*isolation & purification, Ketones, Lactobacillus/*analysis, Spectrophotometry/methods, TetraphenylborateEnzymology
Enzymology4673708Serological studies on the teichoic acids of Lactobacillus plantarum.Knox KW, Wicken AJInfect Immun10.1128/iai.6.1.43-49.19721972Allergy and Immunology, Animals, Antibodies, Bacterial/analysis, Antibody Specificity, *Antigens, Bacterial/analysis, Cell Membrane/immunology, Cell Wall/immunology, Chromatography, Cross Reactions, *Glycosides, Immunoglobulin G/analysis/isolation & purification, Immunoglobulin M/analysis/isolation & purification, Lactobacillus/cytology/*immunology, Mutation, *Phosphoric Acids, Precipitin Tests, Rabbits/immunology, Sugar Alcohols/analysis, Teichoic AcidsPhylogeny
7141685Teichoic acids from chemostat-grown cultures of Streptococcus mutans and Lactobacillus plantarum.Wicken AJ, Evans JD, Campbell LK, Knox KWInfect Immun10.1128/iai.38.1.1-7.19821982Cell Wall/analysis, Glucose/analysis, Glycerol/analysis, Lactobacillus/*analysis/growth & development, *Lipopolysaccharides, Phosphatidic Acids/*analysis, Phosphorus/analysis, Ribitol/analysis, Streptococcus mutans/*analysis/growth & development, Teichoic Acids/*analysis/immunology
31861054The Effect of the Ultra-High-Pressure Homogenization of Protein Encapsulants on the Survivability of Probiotic Cultures after Spray Drying.Mis-Solval KE, Jiang N, Yuan M, Joo KH, Cavender GAFoods10.3390/foods81206892019
Metabolism31906161Potential Application of Lactic Acid Bacteria to Reduce Aflatoxin B1 and Fumonisin B1 Occurrence on Corn Kernels and Corn Ears.Nazareth TM, Luz C, Torrijos R, Quiles JM, Luciano FB, Manes J, Meca GToxins (Basel)10.3390/toxins120100212019Aflatoxin B1/chemistry/*metabolism, Aspergillus flavus, Biological Phenomena, Culture Media, Food Microbiology, Food Preservation, Fumonisins/chemistry/*metabolism, Fusarium, Hydroxybenzoates/metabolism, Lactic Acid/*metabolism, Lactobacillales/*metabolism, Lactobacillus plantarum/metabolism, Mycotoxins/chemistry/*metabolism, Seeds/chemistry/microbiology, Zea mays/*chemistry/microbiologyCultivation
Biotechnology32919324Optimization of pH, time, temperature, variety and concentration of the added fatty acid and the initial count of added lactic acid Bacteria strains to improve microbial conjugated linoleic acid production in fermented ground beef.Ozer CO, Kilic BMeat Sci10.1016/j.meatsci.2020.1083032020Animals, Cattle, Fatty Acids/chemistry, Fermentation, Food Handling/methods, Hydrogen-Ion Concentration, Lactobacillus plantarum/*metabolism, Linoleic Acids, Conjugated/*biosynthesis, Meat Products/analysis/*microbiology, Safflower Oil, TemperatureMetabolism

Reference

@idauthorscataloguedoi/urltitle
1148Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2601)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2601
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39814Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10610
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
75966Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID265314.1StrainInfo: A central database for resolving microbial strain identifiers
121860Curators of the CIPCollection of Institut Pasteur (CIP 71.39)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2071.39