Strain identifier

BacDive ID: 6492

Type strain: Yes

Species: Lactiplantibacillus pentosus

Strain Designation: 124-2, P26

Strain history: CIP <- 1988, NCFB <- M.E. Sharpe, Reading, UK: strain P26 <- NCIB <- 1950, ATCC <- E.E. Snell: strain 124-2, Lactobacillus plantarum

NCBI tax ID(s): 1423791 (strain), 1589 (species)

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General

@ref: 8592

BacDive-ID: 6492

DSM-Number: 20314

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, rod-shaped, lactate production

description: Lactiplantibacillus pentosus 124-2 is a facultative anaerobe, mesophilic, Gram-positive bacterium that produces lactate and was isolated from Corn silage.

NCBI tax id

NCBI tax idMatching level
1589species
1423791strain

strain history

@refhistory
8592<- ATCC <- E.E. Snell, 124-2 (Lactobacillus plantarum)
67770ATCC 8041 <-- E. E. Snell 124-2.
121666CIP <- 1988, NCFB <- M.E. Sharpe, Reading, UK: strain P26 <- NCIB <- 1950, ATCC <- E.E. Snell: strain 124-2, Lactobacillus plantarum

doi: 10.13145/bacdive6492.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactiplantibacillus
  • species: Lactiplantibacillus pentosus
  • full scientific name: Lactiplantibacillus pentosus (Zanoni et al. 1987 ex Fred et al. 1921) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus pentosus

@ref: 8592

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactiplantibacillus

species: Lactiplantibacillus pentosus

full scientific name: Lactiplantibacillus pentosus (Zanoni et al. 1987) Zheng et al. 2020

strain designation: 124-2, P26

type strain: yes

Morphology

cell morphology

  • @ref: 121666
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8592MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
35498MEDIUM 41- for Lactobacillus, Leuconostoc, Weissella, Pediococcus, Sporolactobacillus inulinusyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp broth (55.000 g)
121666CIP Medium 41yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=41

culture temp

@refgrowthtypetemperature
8592positivegrowth30
35498positivegrowth37
67770positivegrowth30

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
121666facultative anaerobe
125439obligate aerobe96

compound production

@refcompound
8592acetic acid
8592lactic acid

murein

  • @ref: 8592
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

observation

  • @ref: 67770
  • observation: Assay of Biotin, nicotinic and pantothenic acids

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12166617632nitrate-reduction
12166616301nitrite-reduction
68371Potassium 5-ketogluconate-builds acid from
6837127613amygdalin+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose+builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837159640N-acetylglucosamine+builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose+builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose+builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol+builds acid from

metabolite production

  • @ref: 121666
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
121666oxidase-
121666catalase-1.11.1.6
121666urease-3.5.1.5
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121666-+---+----++++-+++--

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8592-+--+++---++++----++--+++++++++++------+----------
8592-+--+++---++++----++--+++++++++++-----------------
8592-+--+++---++++-+/---++--+++++++++++------++/-----+/--+--
8592-+--+++---++++-+/---++--+++++++++++------++/-----+/--+--
121666+--+++---++++-+/---+---+++++++++++------+----------

Isolation, sampling and environmental information

isolation

  • @ref: 67770
  • sample type: Corn silage

taxonmaps

  • @ref: 69479
  • File name: preview.99_195.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_140;97_151;98_168;99_195&stattab=map
  • Last taxonomy: Lactobacillaceae
  • 16S sequence: LC071808
  • Sequence Identity:
  • Total samples: 38637
  • soil counts: 2696
  • aquatic counts: 4479
  • animal counts: 29069
  • plant counts: 2393

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
85921Risk group (German classification)
1216661Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus plantarum ATCC 8041 16S ribosomal RNA gene, partial sequenceAF429521506nuccore1590
20218Lactobacillus plantarum strain ATCC 8041 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF429608516nuccore1590
20218Lactobacillus pentosus gene for 16S rRNA, partial sequenceD792111519nuccore1589
20218Lactobacillus pentosus 16S/23S ribosomal RNA small intergenic spacer region, complete sequenceU97134205nuccore1589
20218Lactobacillus pentosus 16S/23S ribosomal RNA large intergenic spacer region, tRNA-Ile and tRNA-Ala genes, complete sequenceU97140439nuccore1589
20218Lactobacillus pentosus gene for 16S rRNA, partial sequence, strain: JCM 1558AB289240622nuccore1589
67770Lactobacillus pentosus gene for 16S ribosomal RNA, partial sequence, strain: JCM 1558LC0718081460nuccore1589
124043Lactobacillus pentosus gene for 16S rRNA, partial sequence, strain: NBRC 106467.AB6260601492nuccore1589

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactiplantibacillus pentosus DSM 20314GCA_003641185completencbi1589
66792Lactobacillus pentosus DSM 203141423791.3wgspatric1423791
66792Lactobacillus pentosus strain ATCC 80411589.100wgspatric1589
66792Lactobacillus pentosus strain DSM 203141589.90completepatric1589
66792Lactobacillus pentosus strain NBRC 1064671589.102wgspatric1589
66792Lactiplantibacillus pentosus DSM 203142700989129draftimg1423791
66792Lactiplantibacillus pentosus DSM 203142896846605completeimg1423791
67770Lactiplantibacillus pentosus DSM 20314GCA_001433755scaffoldncbi1423791
67770Lactiplantibacillus pentosus NBRC 106467GCA_007991835contigncbi1589
67770Lactiplantibacillus pentosus ATCC 8041GCA_004354685scaffoldncbi1589

GC content

@refGC-contentmethod
859246.1
6777046.1thermal denaturation, midpoint method (Tm)
6777043.8

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidence
125439BacteriaNetspore_formationAbility to form endo- or exosporesyes52.1
125439BacteriaNetmotilityAbility to perform movementyes60.5
125439BacteriaNetgram_stainReaction to gram-stainingpositive77.6
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthobligate aerobe96

External links

@ref: 8592

culture collection no.: DSM 20314, ATCC 8041, DSM 43, NCDO 363, NCIB 8026, CCUG 33455, NCIMB 8026, LMG 10755, JCM 1558, BCRC 11053, CECT 4023, CIP 103156, KCTC 3120, NBRC 106467, NCAIM B.01727, NCCB 32014, NRIC 1068, NRRL B-227, NRRL B-473, VTT E-99168

straininfo link

  • @ref: 75965
  • straininfo: 294493

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Biotechnology15610436Characterization of an acid phosphatase from Lactobacillus pentosus: regulation and biochemical properties.Palacios MC, Haros M, Rosell CM, Sanz YJ Appl Microbiol10.1111/j.1365-2672.2004.02447.x20056-Phytase/analysis, Acid Phosphatase/*analysis, Bacteriological Techniques, Fermentation, *Food Microbiology, Hydrogen-Ion Concentration, Lactobacillus/*enzymology, Spectrophotometry, TemperatureEnzymology
Pathogenicity23182040Improved acid tolerance of Lactobacillus pentosus by error-prone whole genome amplification.Ye L, Zhao H, Li Z, Wu JCBioresour Technol10.1016/j.biortech.2012.10.0422012Acids/*pharmacology, Adaptation, Physiological/drug effects/*genetics, Biomass, Bioreactors/microbiology, Genome, Bacterial/*genetics, Glucose/metabolism, Hydrogen-Ion Concentration/drug effects, L-Lactate Dehydrogenase/metabolism, Lactic Acid/biosynthesis, Lactobacillus/drug effects/*genetics/growth & development/*physiology, Mutation/genetics, Polymerase Chain Reaction/*methodsGenetics
Phylogeny23950151Lactobacillus mudanjiangensis sp. nov., Lactobacillus songhuajiangensis sp. nov. and Lactobacillus nenjiangensis sp. nov., isolated from Chinese traditional pickle and sourdough.Gu CT, Li CY, Yang LJ, Huo GCInt J Syst Evol Microbiol10.1099/ijs.0.054296-02013Bacterial Typing Techniques, Base Composition, Bread/microbiology, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fermentation, *Food Microbiology, Genes, Bacterial, Lactobacillus/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vegetables/microbiologyBiotechnology
Enzymology26801669Cloning and characterization of two distinct water-forming NADH oxidases from Lactobacillus pentosus for the regeneration of NAD.Zhang JD, Cui ZM, Fan XJ, Wu HL, Chang HHBioprocess Biosyst Eng10.1007/s00449-016-1542-82016*Bacterial Proteins/biosynthesis/chemistry/genetics, Catalysis, Cloning, Molecular, Hydrogen-Ion Concentration, *Lactobacillus pentosus/enzymology/genetics, NAD/genetics/*metabolism, *NADPH Oxidases/biosynthesis/chemistry/genetics, Oxidation-ReductionMetabolism
Pathogenicity27904056Prophylactic Effect of Lactobacillus pentosus strain S-PT84 on Candida Infection and Gastric Inflammation in a Murine Gastrointestinal Candidiasis Model.Maekawa T, Ishijima AS, Ida M, Izumo T, Ono Y, Shibata H, Abe SMed Mycol J10.3314/mmj.16-000122016Administration, Oral, Animals, *Antibiosis, Candida albicans/*growth & development, Candidiasis/*prevention & control, Capillary Permeability, Disease Models, Animal, Female, Gastrointestinal Diseases/*prevention & control, Inflammation/*prevention & control, *Lactobacillus pentosus, Mice, Inbred ICR, *Neutrophil Infiltration, Probiotics/*administration & dosage, Severity of Illness Index, Stomach/microbiology
Pathogenicity27904074Prophylactic Effect of Lactobacillus pentosus strain S-PT84 on Candida Infection and Gastric Inflammation in a Murine Gastrointestinal Candidiasis Model [Errata].Maekawa T, Ishijima AS, Ida M, Izumo T, Ono Y, Shibata H, Abe SMed Mycol J10.3314/mmj.16-00012E2016Administration, Oral, Animals, *Antibiosis, Candida albicans/*growth & development, Candidiasis/*prevention & control, Capillary Permeability, Disease Models, Animal, Female, Gastrointestinal Diseases/*prevention & control, Inflammation/*prevention & control, *Lactobacillus pentosus, Mice, Inbred ICR, *Neutrophil Infiltration, Probiotics/*administration & dosage, Severity of Illness Index, Stomach/microbiology
Metabolism30166164Expression of genes involved in metabolism of phenolic compounds by Lactobacillus pentosus and its relevance for table-olive fermentations.Carrasco JA, Lucena-Padros H, Brenes M, Ruiz-Barba JLFood Microbiol10.1016/j.fm.2018.06.0202018Bacterial Proteins/*genetics/metabolism, Carboxy-Lyases/genetics/metabolism, Carboxylic Ester Hydrolases/genetics/metabolism, Fermentation, Gallic Acid/analogs & derivatives/metabolism, Glucosidases/genetics/metabolism, Lactobacillus pentosus/enzymology/*genetics/*metabolism, Olea/chemistry/metabolism/*microbiology, Phenols/*metabolismEnzymology
Metabolism30378762Lactobacillus pentosus CECT 4023 T co-utilizes glucose and xylose to produce lactic acid from wheat straw hydrolysate: Anaerobiosis as a key factor.Cubas-Cano E, Gonzalez-Fernandez C, Ballesteros M, Tomas-Pejo EBiotechnol Prog10.1002/btpr.27392018Anaerobiosis, Fermentation/physiology, Glucose/metabolism, Hydrolysis, Lactic Acid/*metabolism, Lactobacillus pentosus/*metabolism, Triticum/*metabolism
Biotechnology31174085Evolutionary engineering of Lactobacillus pentosus improves lactic acid productivity from xylose-rich media at low pH.Cubas-Cano E, Gonzalez-Fernandez C, Tomas-Pejo EBioresour Technol10.1016/j.biortech.2019.1215402019Fermentation, Hydrogen-Ion Concentration, Lactic Acid, *Lactobacillus pentosus, *Xylose
Metabolism32036453Optimization of immobilization conditions for Lactobacillus pentosus cells.Wang J, Huang J, Guo H, Jiang S, Zhang J, Ning Y, Fang M, Liu SBioprocess Biosyst Eng10.1007/s00449-020-02305-92020Alginates/*chemistry, Cells, Immobilized/*metabolism, Lactic Acid/*metabolism, Lactobacillus pentosus/*metabolism, Polyvinyl Alcohol/*chemistry
Phylogeny32228805Lactobacillus garii sp. nov., isolated from a fermented cassava product.Diaz M, Sayavedra L, Atter A, Mayer MJ, Saha S, Amoa-Awua W, Narbad AInt J Syst Evol Microbiol10.1099/ijsem.0.0041212020Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fermented Foods/*microbiology, *Food Microbiology, Genes, Bacterial, Ghana, Lactobacillus/*classification/isolation & purification, Manihot/*microbiology, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNABiotechnology
Biotechnology32325410Efficient utilization of hydrolysates from steam-exploded gardening residues for lactic acid production by optimization of enzyme addition and pH control.Cubas-Cano E, Gonzalez-Fernandez C, Ballesteros I, Tomas-Pejo EWaste Manag10.1016/j.wasman.2020.04.0032020Fermentation, Gardening, Hydrogen-Ion Concentration, Hydrolysis, *Lactic Acid, *Steam, XyloseEnzymology
Pathogenicity36098081Partial Purification of Bacteriocin from Lactobacillus pentosus Strain 124-2 Isolated from "Dadih".Yuliana T, Hayati F, Rialita T, Cahyana YPak J Biol Sci10.3923/pjbs.2022.796.8022022Ammonium Sulfate, *Anti-Infective Agents/pharmacology, *Bacteriocins/pharmacology, Escherichia coli, Food Preservatives/pharmacology, Humans, *Lactobacillus pentosusEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8592Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20314)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20314
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
35498Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14922
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75965Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID294493.1StrainInfo: A central database for resolving microbial strain identifiers
121666Curators of the CIPCollection of Institut Pasteur (CIP 103156)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103156
124043Isabel Schober, Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardyhttps://github.com/GenomeNet/deepGdeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1