Strain identifier

BacDive ID: 6489

Type strain: Yes

Species: Lactobacillus pasteurii

Strain Designation: 1517

Strain history: CIP <- 2008, F. Gasser, Institut Pasteur, Paris, France: strain 1517

NCBI tax ID(s): 1423790 (strain), 872327 (species)

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General

@ref: 17360

BacDive-ID: 6489

DSM-Number: 23907

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, rod-shaped, colony-forming

description: Lactobacillus pasteurii 1517 is a microaerophile, mesophilic, Gram-positive bacterium that forms round colonies.

NCBI tax id

NCBI tax idMatching level
1423790strain
872327species

strain history

@refhistory
17360<- S. Cousin, CIP, Genopole, Paris, France; CRBIP 24.76
67770CRBIP 24.76 <-- F. Gasser 1517.
67771<- DSM <- S Cousin, CIP, Genopole, Paris, France, CRBIP 24.76
121623CIP <- 2008, F. Gasser, Institut Pasteur, Paris, France: strain 1517

doi: 10.13145/bacdive6489.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactobacillus
  • species: Lactobacillus pasteurii
  • full scientific name: Lactobacillus pasteurii Cousin et al. 2013

@ref: 17360

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus pasteurii

full scientific name: Lactobacillus pasteurii Cousin et al. 2013

strain designation: 1517

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
30463positive1.19 µm0.47 µmrod-shapedno
67771rod-shapedno
67771positive
69480no96.544
69480positive100
121623positiverod-shaped

colony morphology

@refcolony colorcolony shapemedium used
121623translucentroundDe Man, Rogosa and Sharpe Agar
121623translucentroundTrypticase Soy Agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17360MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
121623De Man, Rogosa and Sharpe Agaryes
121623Trypticase Soy Agaryes

culture temp

@refgrowthtypetemperaturerange
17360positivegrowth37mesophilic
30463positivegrowth30-45
30463positiveoptimum41thermophilic
67770positivegrowth37mesophilic
67771positivegrowth37mesophilic
121623positivegrowth37-45
121623nogrowth15psychrophilic
121623nogrowth22psychrophilic

culture pH

@refabilitytypepHPH range
30463positivegrowth04-08acidophile
30463positiveoptimum6.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
17360microaerophile
30463facultative anaerobe
67771anaerobe
121623facultative anaerobe

spore formation

@refspore formationconfidence
30463no
67771no
69481no100
69480no99.989

halophily

@refsaltgrowthtested relationconcentration
30463NaClpositivegrowth0-3 %
30463NaClpositiveoptimum1.5 %

murein

  • @ref: 17360
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

observation

  • @ref: 30463
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3046317057cellobiose+carbon source
3046328757fructose+carbon source
3046317234glucose+carbon source
3046317306maltose+carbon source
3046329864mannitol+carbon source
3046337684mannose+carbon source
30463506227N-acetylglucosamine+carbon source
3046333942ribose+carbon source
3046317814salicin+carbon source
3046317992sucrose+carbon source
304634853esculin+hydrolysis
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837128053melibiose-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
12162317632nitrate-reduction
12162316301nitrite-reduction
12162317632nitrate+respiration

metabolite tests

  • @ref: 121623
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
121623oxidase-
121623alcohol dehydrogenase-1.1.1.1
121623catalase-1.11.1.6
121623lysine decarboxylase-4.1.1.18
121623ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121623-+---+----+--+-++---

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
17360-----------+++----+---++++++++-+----+--+----------
17360-----------+++----+---++++++++-+----+--+----------
17360-----------+++----+---++++++++-+----+--+----------
121623----+/------+/-+/-+/-----+/----+/-+/-+/-+/-+/-+/-+/-+/--+/-----+/---+/-----------

Isolation, sampling and environmental information

isolation

@refcountryorigin.countrycontinent
17360FranceFRAEurope
67770
67771FranceFRAEurope

taxonmaps

  • @ref: 69479
  • File name: preview.99_2024.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_46;96_1120;97_1315;98_1585;99_2024&stattab=map
  • Last taxonomy: Lactobacillus pasteurii
  • 16S sequence: LC589212
  • Sequence Identity:
  • Total samples: 593
  • soil counts: 21
  • aquatic counts: 39
  • animal counts: 526
  • plant counts: 7

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
173601Risk group (German classification)
1216231Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
17360Lactobacillus pasteurii partial 16S rRNA gene, strain 1517FR6819011426ena1423790
67770Lactobacillus pasteurii JCM 18989 gene for 16S rRNA, partial sequenceLC5892121500ena872327

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus pasteurii CRBIP 24.761203066.3wgspatric1423790
66792Lactobacillus pasteurii DSM 23907 = CRBIP 24.761423790.3wgspatric1423790
66792Lactobacillus pasteurii strain DSM 23907872327.3wgspatric872327
66792Lactobacillus pasteurii DSM 239072916475386draftimg1423790
66792Lactobacillus pasteurii CRBIP 24.762563366755draftimg1423790
66792Lactobacillus pasteurii DSM 239072660238722draftimg1423790
67770Lactobacillus pasteurii DSM 23907 = CRBIP 24.76GCA_001433915contigncbi1423790
67770Lactobacillus pasteurii DSM 23907GCA_004354755contigncbi872327
67771Lactobacillus pasteurii DSM 23907 = CRBIP 24.76 type strain:CRBIP 24.76GCA_000297025contigncbi1423790

GC content

@refGC-contentmethod
1736045.4high performance liquid chromatography (HPLC)
3046345.4
6777038.5genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno96.391yes
gram-positiveyes96.244yes
anaerobicyes59.267no
aerobicno98.365yes
halophileyes60.394yes
spore-formingno97.333yes
thermophileno99.182no
glucose-utilyes93.347yes
flagellatedno98.433yes
glucose-fermentyes91.856no

External links

@ref: 17360

culture collection no.: DSM 23907, KCTC 21048, JCM 18989, CRBIP24.76

straininfo link

  • @ref: 75962
  • straininfo: 386785

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny22328611Lactobacillus pasteurii sp. nov. and Lactobacillus hominis sp. nov.Cousin S, Motreff L, Gulat-Okalla ML, Gouyette C, Sproer C, Schumann P, Begaud E, Bouchier C, Clermont D, Bizet CInt J Syst Evol Microbiol10.1099/ijs.0.036665-02012Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Lactobacillus/*classification/genetics/metabolism, Molecular Sequence Data, Multilocus Sequence Typing, Nucleic Acid Hybridization, Peptidoglycan/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics
Genetics23969061Draft Genome Sequence of Lactobacillus pasteurii CRBIP 24.76T.Cousin S, Clermont D, Creno S, Ma L, Loux V, Bizet C, Bouchier CGenome Announc10.1128/genomeA.00660-132013
Phylogeny34264810Lactobacillus corticis sp. nov., isolated from hardwood bark.Tohno M, Tanizawa Y, Kojima Y, Sakamoto M, Ohkuma M, Kobayashi HInt J Syst Evol Microbiol10.1099/ijsem.0.0048822021Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Japan, Lactobacillus/*classification/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, Plant Bark/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
17360Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23907)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23907
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30463Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2679828776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
75962Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID386785.1StrainInfo: A central database for resolving microbial strain identifiers
121623Curators of the CIPCollection of Institut Pasteur (CRBIP24.76 )https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CRBIP24.76%20