Strain identifier

BacDive ID: 6476

Type strain: No

Species: Lentilactobacillus kefiri

Strain Designation: Ke 10, Ke10

Strain history: CIP <- 2000, F. Gasser, Inst. Pasteur, Paris, France, L. kefiri <- NCDO <- NCIB, "Lactobacillus caucasicus" <- 1954, M.E. Sharpe, NIRD, Lactobacillus brevis <- C.S. Pederson: strain Ke10

NCBI tax ID(s): 33962 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 9.2 (current version):
version 9:
version 8.1:
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 9.2 (current version)

General

@ref: 8731

BacDive-ID: 6476

DSM-Number: 20485

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, rod-shaped

description: Lentilactobacillus kefiri Ke 10 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from kefir grains.

NCBI tax id

  • NCBI tax id: 33962
  • Matching level: species

strain history

@refhistory
8731<- NCDO <- NCIB <- C.S. Pederson, Ke 10
117068CIP <- 2000, F. Gasser, Inst. Pasteur, Paris, France, L. kefiri <- NCDO <- NCIB, "Lactobacillus caucasicus" <- 1954, M.E. Sharpe, NIRD, Lactobacillus brevis <- C.S. Pederson: strain Ke10

doi: 10.13145/bacdive6476.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lentilactobacillus
  • species: Lentilactobacillus kefiri
  • full scientific name: Lentilactobacillus kefiri (Kandler and Kunath 1983) Zheng et al. 2020

  • synonyms

    @refsynonym
    20215Lactobacillus kefiri
    20215Lactobacillus kefir

@ref: 8731

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lentilactobacillus

species: Lentilactobacillus kefiri

full scientific name: Lentilactobacillus kefiri (Kandler and Kunath 1983) Zheng et al. 2020

strain designation: Ke 10, Ke10

type strain: no

Morphology

cell morphology

  • @ref: 117068
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8731MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
117068CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperature
8731positivegrowth30
117068positivegrowth22-45
117068nogrowth10
117068nogrowth15

Physiology and metabolism

oxygen tolerance

  • @ref: 117068
  • oxygen tolerance: facultative anaerobe

murein

  • @ref: 8731
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11706817632nitrate-reduction
11706816301nitrite-reduction
11706817632nitrate+respiration
68371Potassium 5-ketogluconate-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate+builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose+builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837130849L-arabinose+builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from

antibiotic resistance

  • @ref: 117068
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite tests

  • @ref: 117068
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-chymotrypsin-3.4.21.1
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)-
117068oxidase-
117068alcohol dehydrogenase+1.1.1.1
117068catalase-1.11.1.6
117068lysine decarboxylase-4.1.1.18
117068ornithine decarboxylase-4.1.1.17
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
117068---+-++---++++-++---

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8731----++/-----++/-+/----------------+++----+/------------+--

Isolation, sampling and environmental information

isolation

@refsample type
8731kefir grains
117068Plant, Kefir grains

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
87311Risk group (German classification)
1170681Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus brevis ATCC 8007 16S ribosomal RNA gene, partial sequenceAF429497506nuccore1580
20218Lactobacillus brevis strain ATCC 8007 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF429584530nuccore1580
20218Lactobacillus kefiri 16S-23S ribosomal RNA intergenic spacer, complete sequenceAY821849222nuccore33962

GC content

  • @ref: 8731
  • GC-content: 42.7

External links

@ref: 8731

culture collection no.: DSM 20485, ATCC 8007, NCDO 190, NCIB 8120, CIP 106379, NCIMB 8120

straininfo link

  • @ref: 75949
  • straininfo: 3319

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16433957Molecular identification and typing of lactobacilli isolated from kefir grains.Delfederico L, Hollmann A, Martinez M, Iglesias NG, De Antoni G, Semorile LJ Dairy Res10.1017/S00220299050014082006Bacterial Typing Techniques, Base Sequence, Cultured Milk Products/*microbiology, DNA Primers, DNA Restriction Enzymes, DNA, Bacterial/*analysis/genetics, Genotype, Lactobacillus/*classification/genetics/*isolation & purification, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Random Amplified Polymorphic DNA Technique/*methods, Sequence Analysis, DNA, Species SpecificityGenetics
Metabolism22126976Surface proteins from Lactobacillus kefir antagonize in vitro cytotoxic effect of Clostridium difficile toxins.Carasi P, Trejo FM, Perez PF, De Antoni GL, Serradell Mde LAnaerobe10.1016/j.anaerobe.2011.11.0022011Animals, Antibiosis, Bacterial Toxins/*antagonists & inhibitors/metabolism, Cell Line, Chlorocebus aethiops, Clostridioides difficile/*metabolism, Lactobacillus/*metabolism, Membrane Glycoproteins/*metabolism, Protein BindingPathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8731Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20485)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20485
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
75949Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3319.1StrainInfo: A central database for resolving microbial strain identifiers
117068Curators of the CIPCollection of Institut Pasteur (CIP 106379)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106379