Strain identifier

BacDive ID: 6475

Type strain: Yes

Species: Lactobacillus jensenii

Strain Designation: 62G

Strain history: CIP <- 1969, F. Gasser, Inst. Pasteur, Paris, France: strain 62G

NCBI tax ID(s): 1423762 (strain), 109790 (species)

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General

@ref: 8907

BacDive-ID: 6475

DSM-Number: 20557

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped, human pathogen

description: Lactobacillus jensenii 62G is an anaerobe, mesophilic, Gram-positive human pathogen that was isolated from human vaginal discharge.

NCBI tax id

NCBI tax idMatching level
109790species
1423762strain

strain history

@refhistory
8907<- ATCC <- F. Gasser, 62G
349171969, F. Gasser, Inst. Pasteur, Paris, France: strain 62G
67770LMG 6414 <-- DSM 20557 <-- ATCC 25258 <-- F. Gasser 62G.
120341CIP <- 1969, F. Gasser, Inst. Pasteur, Paris, France: strain 62G

doi: 10.13145/bacdive6475.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactobacillus
  • species: Lactobacillus jensenii
  • full scientific name: Lactobacillus jensenii Gasser et al. 1970 (Approved Lists 1980)

@ref: 8907

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus jensenii

full scientific name: Lactobacillus jensenii Gasser et al. 1970

strain designation: 62G

type strain: yes

Morphology

cell morphology

  • @ref: 120341
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
34917MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
8907MRS pre-reduced (DSMZ Medium 11b)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium11b.pdf
120341CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
8907positivegrowth30mesophilic
34917positivegrowth37mesophilic
67770positivegrowth37mesophilic
120341positivegrowth25-45
120341nogrowth15psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8907anaerobe
8907microaerophile

murein

  • @ref: 8907
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
120341nitrate-reduction17632
120341nitrite-reduction16301

metabolite tests

  • @ref: 120341
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
120341oxidase-
120341alcohol dehydrogenase-1.1.1.1
120341catalase-1.11.1.6
120341lysine decarboxylase-4.1.1.18
120341ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120341-+---+----++---++---

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8907-----+/------+++---------++++++--++------+----------
8907-----------+++-----------++----++-----------------
8907-----+/-----+/-+++---------++++++--++------+----------
8907-----+/------+++---------++++++--++---+/---+/-----------

Isolation, sampling and environmental information

isolation

@refsample type
8907human vaginal discharge
67770Human vaginal discharge
120341Human, Vaginal discharge

isolation source categories

Cat1Cat2Cat3
#Host#Human#Female
#Host Body Product#Urogenital tract#Vaginal secretion

taxonmaps

  • @ref: 69479
  • File name: preview.99_115.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_46;96_90;97_95;98_104;99_115&stattab=map
  • Last taxonomy: Lactobacillus
  • 16S sequence: AF243176
  • Sequence Identity:
  • Total samples: 46199
  • soil counts: 816
  • aquatic counts: 1287
  • animal counts: 43559
  • plant counts: 537

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
8907yes, in single cases1Risk group (German classification)
1203411Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus jensenii strain ATCC25258 16S ribosomal RNA gene, partial sequenceAF2431761496ena109790
20218Lactobacillus jensenii ATCC 25258 16S ribosomal RNA gene, partial sequenceAF429513504ena109790
20218Lactobacillus jensenii strain ATCC 25258 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF429600516ena109790
20218Lactobacillus jensenii gene, 16S-23S rRNA intergenic spacer region and 23S rRNA gene 5' flanking region, partial sequenceAB035486644ena109790

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus jensenii ATCC 25258GCA_018094625contigncbi109790
66792Lactobacillus jensenii DSM 205571423762.3wgspatric1423762
66792Lactobacillus jensenii strain ATCC 25258109790.239wgspatric109790
66792Lactobacillus jensenii DSM 205572657245084draftimg1423762
67770Lactobacillus jensenii DSM 20557GCA_001436455scaffoldncbi1423762

GC content

@refGC-contentmethod
890736.1
6777034.33genome sequence analysis
6777036.1Buoyant density centrifugation (BD)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno95.135no
gram-positiveyes96.741no
anaerobicyes81.648no
halophileyes75.763no
spore-formingno97.958no
glucose-utilyes92.638no
thermophileno95.608no
aerobicno97.989yes
flagellatedno98.113no
glucose-fermentyes91.854no

External links

@ref: 8907

culture collection no.: DSM 20557, ATCC 25258, JCM 15953, BCRC 12939, CCUG 21961, CCUG 35572, CECT 4306, CIP 69.17, JCM 1146, KCTC 3599, KCTC 5194, LMG 6414, NCFB 2165, NCIMB 13279, NRRL B-4550, NCDO 2165

straininfo link

  • @ref: 75948
  • straininfo: 3316

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10843070Lactobacillus fornicalis sp. nov., isolated from the posterior fornix of the human vagina.Dicks LM, Silvester M, Lawson PA, Collins MDInt J Syst Evol Microbiol10.1099/00207713-50-3-12532000Bacterial Proteins/chemistry, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Female, Genes, rRNA, Humans, Lactobacillus/*classification/genetics/isolation & purification, Molecular Sequence Data, Phenotype, *Phylogeny, Polymerase Chain Reaction, RNA, Ribosomal, 16S/genetics, Random Amplified Polymorphic DNA Technique, Sequence Analysis, DNA, Vagina/*microbiologyPathogenicity
Enzymology17184325Development of antioxidant activity in milk whey during fermentation with lactic acid bacteria.Virtanen T, Pihlanto A, Akkanen S, Korhonen HJ Appl Microbiol10.1111/j.1365-2672.2006.03072.x2007Amino Acids/analysis, Animals, Antioxidants/*metabolism, Bifidobacterium/growth & development/isolation & purification, Colony Count, Microbial, Fermentation/*physiology, *Food Microbiology, Gram-Positive Bacteria/*growth & development/isolation & purification, Lactobacillus/growth & development/isolation & purification, Lactococcus/growth & development/isolation & purification, Leuconostoc/growth & development/isolation & purification, Lipid Peroxidation, Milk/*microbiology, Milk Proteins/*metabolism, Molecular Weight, Peptide Hydrolases/metabolism, Reactive Oxygen Species/metabolism, Whey ProteinsPhylogeny
Metabolism19948869Biosynthesis and degradation of H2O2 by vaginal lactobacilli.Martin R, Suarez JEAppl Environ Microbiol10.1128/AEM.01631-092009Female, Humans, Hydrogen Peroxide/analysis/*metabolism/pharmacology, Iron/pharmacology, Lactobacillus/drug effects/*metabolism, Middle Aged, Premenopause, Vagina/*microbiologyPathogenicity
Phylogeny24813010Inhibitory effects of seaweed extracts on the growth of the vaginal bacterium Gardnerella vaginalis.Ha YM, Choi JS, Lee BB, Moon HE, Cho KK, Choi ISJ Environ Biol2014Anti-Bacterial Agents/chemistry/*pharmacology, Gardnerella vaginalis/*drug effects, Plant Extracts/chemistry/*pharmacology, Seaweed/*chemistry/classification, Species SpecificityPathogenicity
Metabolism25635270Effect of iron on the probiotic properties of the vaginal isolate Lactobacillus jensenii CECT 4306.Martin R, Sanchez B, Urdaci MC, Langella P, Suarez JE, Bermudez-Humaran LGMicrobiology (Reading)10.1099/mic.0.0000442015Bacterial Adhesion, Bacterial Proteins, Cell Line, Female, Ferric Compounds/metabolism, Humans, Immunomodulation, Iron/*metabolism, Lactobacillus/*metabolism, Microbiota, Mucous Membrane/immunology/microbiology, *Probiotics, Proteome, Proteomics, Vagina/immunology/*microbiologyPathogenicity
Phylogeny34264810Lactobacillus corticis sp. nov., isolated from hardwood bark.Tohno M, Tanizawa Y, Kojima Y, Sakamoto M, Ohkuma M, Kobayashi HInt J Syst Evol Microbiol10.1099/ijsem.0.0048822021Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Japan, Lactobacillus/*classification/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, Plant Bark/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome
Pathogenicity34948426Engineering of Vaginal Lactobacilli to Express Fluorescent Proteins Enables the Analysis of Their Mixture in Nanofibers.Stojanov S, Plavec TV, Kristl J, Zupancic S, Berlec AInt J Mol Sci10.3390/ijms2224136312021Female, Green Fluorescent Proteins/genetics, Humans, Lactobacillus/*genetics, Lactobacillus crispatus/genetics, Lactobacillus gasseri/genetics, Lactobacillus plantarum, Luminescent Proteins/*genetics, *Microorganisms, Genetically-Modified, *Probiotics, Vagina/*microbiologyBiotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8907Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20557)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20557
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34917Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10427
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75948Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3316.1StrainInfo: A central database for resolving microbial strain identifiers
120341Curators of the CIPCollection of Institut Pasteur (CIP 69.17)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2069.17