Strain identifier

BacDive ID: 6473

Type strain: Yes

Species: Lentilactobacillus hilgardii

Strain Designation: 9

Strain history: CIP <- 1987, DSM <- ATCC <- R.H. Vaughn: strain 9

NCBI tax ID(s): 1588 (species)

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General

@ref: 8606

BacDive-ID: 6473

DSM-Number: 20176

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, rod-shaped

description: Lentilactobacillus hilgardii 9 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from wine.

NCBI tax id

  • NCBI tax id: 1588
  • Matching level: species

strain history

@refhistory
8606<- ATCC <- R.H. Vaughn, 9
67770ATCC 8290 <-- R. H. Vaughn 9.
119893CIP <- 1987, DSM <- ATCC <- R.H. Vaughn: strain 9

doi: 10.13145/bacdive6473.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lentilactobacillus
  • species: Lentilactobacillus hilgardii
  • full scientific name: Lentilactobacillus hilgardii (Douglas and Cruess 1936) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus hilgardii

@ref: 8606

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lentilactobacillus

species: Lentilactobacillus hilgardii

full scientific name: Lentilactobacillus hilgardii (Douglas and Cruess 1936) Zheng et al. 2020

strain designation: 9

type strain: yes

Morphology

cell morphology

  • @ref: 119893
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8606MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
34496MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
119893CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
8606positivegrowth30mesophilic
34496positivegrowth30mesophilic
49934positivegrowth37mesophilic
67770positivegrowth30mesophilic
119893positivegrowth15-37
119893nogrowth10psychrophilic
119893nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 119893
  • oxygen tolerance: facultative anaerobe

murein

  • @ref: 8606
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371lactose-builds acid from17716
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
119893nitrate-reduction17632
119893nitrite-reduction16301
119893nitrate+respiration17632

antibiotic resistance

  • @ref: 119893
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite tests

  • @ref: 119893
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
119893oxidase-
119893alcohol dehydrogenase+1.1.1.1
119893catalase-1.11.1.6
119893lysine decarboxylase-4.1.1.18
119893ornithine decarboxylase-4.1.1.17

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    49934C14:01.914
    49934C16:031.516
    49934C18:02.618
    49934C16:1 ω7c2.115.819
    49934C16:1 ω7c/C15:0 ISO 2OH2.115.85
    49934C18:1 ω7c /12t/9t24.917.824
    49934C18:1 ω9c8.217.769
    49934C19:0 CYCLO ω8c21.718.9
    49934C19:0 CYCLO ω9c3.818.87
    49934Unidentified1.213.242
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119893--++-+-+--++++-+----

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8606-----++---+++---------------+-+-------------------
8606-----++----+-------------+--+---------------------
8606-----++-----+------------+---------------------+--
119893----++---+++---------------+-+----------------+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119893---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample type
8606wine
49934Wine
67770Wine
119893Plant, Wine

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Beverage
#Engineered#Food production#Fermented
#Host#Plants#Shrub (Scrub)
#Host Body-Site#Plant#Fruit (Seed)

taxonmaps

  • @ref: 69479
  • File name: preview.99_4716.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_367;97_831;98_1249;99_4716&stattab=map
  • Last taxonomy: Lentilactobacillus hilgardii
  • 16S sequence: LC064898
  • Sequence Identity:
  • Total samples: 2549
  • soil counts: 198
  • aquatic counts: 277
  • animal counts: 1773
  • plant counts: 301

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
86061Risk group (German classification)
1198931Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus hilgardii ATCC 8290 16S ribosomal RNA gene, partial sequenceAF429510513ena1588
20218Lactobacillus hilgardii strain ATCC 8290 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF429597544ena1588
20218Lactobacillus hilgardii strain ATCC 8290 16S-23S ribosomal RNA intergenic spacer and 23S ribosomal RNA gene, partial sequenceEU161617834ena1588
20218Lactobacillus hilgardii strain DSM 20176 16S ribosomal RNA gene, partial sequenceM588211521ena1588
20218Lactobacillus hilgardii gene for 16S rRNA, partial sequence, strain: JCM 1155AB289158630ena1588
20218Lactobacillus hilgardii gene for 16S rRNA, partial sequence, strain: YIT 0269AB4293701566ena1588
20218Lactobacillus hilgardii gene for 16S ribosomal RNA, partial sequenceD31693227ena1588
20218Lactobacillus hilgardii gene for 16S rRNA, partial sequence, strain: NBRC 15886AB6809891499ena1588
67770Lactobacillus hilgardii gene for 16S rRNA, partial sequenceAB2629621549ena1588
67770Lactobacillus hilgardii gene for 16S ribosomal RNA, partial sequence, strain: JCM 1155LC0648981523ena1588

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus hilgardii ATCC 8290525327.3wgspatric1423757
66792Lactobacillus hilgardii DSM 20176 = ATCC 82901423757.3wgspatric1423757
66792Lactobacillus hilgardii strain ATCC 82901588.22wgspatric1588
66792Lentilactobacillus hilgardii ATCC 8290643886044draftimg1423757
66792Lentilactobacillus hilgardii DSM 201762660237994draftimg1423757
67770Lentilactobacillus hilgardii DSM 20176 = ATCC 8290GCA_000159315scaffoldncbi1423757
67770Lentilactobacillus hilgardii DSM 20176 = ATCC 8290GCA_001434655contigncbi1423757
67770Lentilactobacillus hilgardii ATCC 8290GCA_004354795scaffoldncbi1588

GC content

@refGC-contentmethod
860640.3
6777039.6genome sequence analysis
6777040

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveyes96.672no
anaerobicno89.92no
halophileyes82.431no
spore-formingno92.779no
glucose-utilyes89.498no
motileno93.93no
flagellatedno98.03no
aerobicno94.439no
thermophileno98.953no
glucose-fermentyes86.037no

External links

@ref: 8606

culture collection no.: DSM 20176, ATCC 8290, NCDO 264, NCIB 8040, CCUG 30140, LMG 6895, JCM 1155, BCRC 12937, CECT 4786, CIP 103007, IFO 15886, KCTC 3500, NBIMCC 3445, NBRC 15886, NCFB 264, NCIMB 8040, NRIC 1060, NRRL B-1843, VTT E-991266

straininfo link

  • @ref: 75946
  • straininfo: 92309

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Biotechnology2246138Lactobacilli isolated from sugary kefir grains capable of polysaccharide production and minicell formation.Pidoux M, Marshall VM, Zanoni P, Brooker BJ Appl Bacteriol10.1111/j.1365-2672.1990.tb01521.x1990Animals, DNA, Bacterial/analysis, *Food Microbiology, Hydrogen-Ion Concentration, Lactobacillus/*classification/genetics/growth & development/metabolism, Microscopy, Electron, *Milk, Nucleic Acid Hybridization, Phenotype, Polysaccharides, Bacterial/*biosynthesis, TemperaturePhylogeny
Metabolism12428963Mousy off-flavor of wine: precursors and biosynthesis of the causative N-heterocycles 2-ethyltetrahydropyridine, 2-acetyltetrahydropyridine, and 2-acetyl-1-pyrroline by Lactobacillus hilgardii DSM 20176.Costello PJ, Henschke PAJ Agric Food Chem10.1021/jf020341r2002Acetaldehyde/analysis/metabolism, Acylation, Cations, Divalent, Deuterium, Ethanol/analysis/metabolism, Fructose/analysis/metabolism, Heterocyclic Compounds/analysis/chemistry/*metabolism, Lactobacillus/*metabolism, Lysine/analysis/metabolism, Metals, Ornithine/analysis/metabolism, Pyridines/analysis/chemistry/*metabolism, Pyrroles/analysis/chemistry/*metabolism, *Taste, Wine/*analysis
Genetics22465289Insights into the completely annotated genome of Lactobacillus buchneri CD034, a strain isolated from stable grass silage.Heinl S, Wibberg D, Eikmeyer F, Szczepanowski R, Blom J, Linke B, Goesmann A, Grabherr R, Schwab H, Puhler A, Schluter AJ Biotechnol10.1016/j.jbiotec.2012.03.0072012Anaerobiosis, Base Sequence, DNA Transposable Elements, *Genome, Bacterial, Lactobacillus/*genetics/isolation & purification, Metabolic Networks and Pathways/genetics, Molecular Sequence Annotation, Molecular Sequence Data, Phylogeny, Poaceae/*microbiology, Prophages/genetics, Sequence Analysis, DNA, Silage/*microbiologyPhylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8606Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20176)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20176
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34496Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14757
49934Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 30140)https://www.ccug.se/strain?id=30140
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75946Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92309.1StrainInfo: A central database for resolving microbial strain identifiers
119893Curators of the CIPCollection of Institut Pasteur (CIP 103007)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103007