Strain identifier

BacDive ID: 6471

Type strain: Yes

Species: Lactobacillus helveticus

Strain Designation: 12, Lh12

Strain history: CIP <- 1988, DSM <- ATCC <- P.A. Hansen: strain Lh12 <- Orla-Jensen, Thermobacterium helveticum

NCBI tax ID(s): 585520 (strain), 1587 (species)

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General

@ref: 8503

BacDive-ID: 6471

DSM-Number: 20075

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic

description: Lactobacillus helveticus 12 is an anaerobe, mesophilic bacterium that was isolated from emmental cheese.

NCBI tax id

NCBI tax idMatching level
585520strain
1587species

strain history

@refhistory
8503<- ATCC <- P.A. Hansen, Lh12 <- S. Orla-Jensen, 12 (Thermobacterium helveticum)
67770ATCC 15009 <-- P. A. Hansen Lh12 <-- S. Orla-Jensen 12 ("Thermobacterium helveticum").
119869CIP <- 1988, DSM <- ATCC <- P.A. Hansen: strain Lh12 <- Orla-Jensen, Thermobacterium helveticum

doi: 10.13145/bacdive6471.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactobacillus
  • species: Lactobacillus helveticus
  • full scientific name: Lactobacillus helveticus (Orla-Jensen 1919) Bergey et al. 1925 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Thermobacterium helveticum
    20215Lactobacillus suntoryeus

@ref: 8503

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus helveticus

full scientific name: Lactobacillus helveticus (Orla-Jensen 1919) Bergey et al. 1925

strain designation: 12, Lh12

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no93.976
69480100positive
119869nopositiverod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8503MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
37298MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
119869CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
8503positivegrowth37mesophilic
37298positivegrowth37mesophilic
49933positivegrowth37mesophilic
67770positivegrowth37mesophilic
119869positivegrowth30-45
119869nogrowth10psychrophilic
119869nogrowth15psychrophilic
119869nogrowth22psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8503anaerobe
8503microaerophile
49933anaerobe
49933microaerophile
119869facultative anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.997

murein

  • @ref: 8503
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
119869nitrate-reduction17632
119869nitrite-reduction16301
119869nitrate+respiration17632

metabolite tests

  • @ref: 119869
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
119869oxidase-
119869beta-galactosidase+3.2.1.23
119869alcohol dehydrogenase-1.1.1.1
119869catalase-1.11.1.6
119869lysine decarboxylase-4.1.1.18
119869ornithine decarboxylase-4.1.1.17

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8503----------++++--------+-----++--+-----------------
8503----------++++--------+-----++--+-----------------
8503----------++-+/---------+------+--+-----------------
8503----------++++--------+------+--+-----------------
8503----------++++--------+------+--+-----------------
8503----------++++--------+-----+/-+--+-----------------
8503----------++++--------+-----++--+-----------------
8503----------++++--------+-----+/-+--+-----------------

Isolation, sampling and environmental information

isolation

@refsample type
8503emmental cheese
49933Emmental cheese
67770Emmental (Swiss) cheese
119869Food, Cheese, Emmental Swiss

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Dairy product

taxonmaps

  • @ref: 69479
  • File name: preview.99_206.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_46;96_146;97_157;98_175;99_206&stattab=map
  • Last taxonomy: Lactobacillus
  • 16S sequence: LC062899
  • Sequence Identity:
  • Total samples: 47444
  • soil counts: 2173
  • aquatic counts: 2250
  • animal counts: 42256
  • plant counts: 765

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
85031Risk group (German classification)
1198691Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus helveticus gene for 16S rRNA, partial sequence, strain: YIT 0083 (= ATCC 15009)AB0082101552ena1587
20218Lactobacillus helveticus strain ATCC 15009; CRL 974 16S ribosomal RNA gene, partial sequenceAF213704525ena585520
20218Lactobacillus helveticus ATCC 15009 16S ribosomal RNA gene, partial sequenceAF429509504ena585520
20218Lactobacillus helveticus strain ATCC 15009 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF429596540ena585520
20218Lactobacillus helveticus strain LMG 6413 16S-23S ribosomal RNA intergenic spacer, complete sequenceDQ123574203ena1587
20218L.helveticus rrn operon, 16S-23S rRNA spacer (long), tRNA-Ile and tRNA-Ala genesZ75482455ena585520
20218L.helveticus rrn operon, 16S-23S rRNA spacer (short)Z75483204ena585520
20218Lactobacillus helveticus partial 16S rRNA gene, type strain DSM 20075TFR6830851516ena1587
20218Lactobacillus helveticus partial 16S rRNA gene, strain type strain:DSM 20075AM1137791554ena1587
20218Lactobacillus helveticus gene for 16S rRNA, partial sequence, strain: JCM 1120AB289155651ena1587
20218Lactobacillus helveticus 16S ribosomal RNA, partial sequence; 16S/23S intergenic spacer region, complete sequence; and 23S ribosomal RNA partial sequenceAF182728660ena1587
20218Lactobacillus helveticus gene for 16S ribosomal RNA, partial sequenceD31692177ena1587
20218Lactobacillus helveticus gene for 16S rRNA, partial sequence, strain: NBRC 15019AB6807511487ena1587
20218L.helveticus 16S rRNA geneX611411333ena1587
20218Lactobacillus helveticus strain NCIMB 11971 16S ribosomal RNA gene, partial sequenceAY3691161529ena1587
67770Lactobacillus helveticus gene for 16S ribosomal RNA, partial sequence, strain: JCM 1120LC0628991463ena1587

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus helveticus JCM 1120GCA_015698225contigncbi1587
66792Lactobacillus helveticus DSM 20075585520.4wgspatric585520
66792Lactobacillus helveticus DSM 20075 = CGMCC 1.1877585520.12wgspatric585520
66792Lactobacillus helveticus DSM 20075 = CGMCC 1.1877 strain ATCC 15009585520.17plasmidpatric585520
66792Lactobacillus helveticus strain JCM 11201587.663wgspatric1587
66792Lactobacillus helveticus DSM 20075645951865draftimg585520
66792Lactobacillus helveticus DSM 200752700989140draftimg585520
67770Lactobacillus helveticus DSM 20075 = CGMCC 1.1877GCA_000160855scaffoldncbi585520
67770Lactobacillus helveticus DSM 20075 = CGMCC 1.1877GCA_001434945scaffoldncbi585520
66792Lactobacillus helveticus DSM 20075 = CGMCC 1.1877GCA_029823695scaffoldncbi585520

GC content

@refGC-contentmethod
850339.3
6777039.3thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno55no
gram-positiveyes93.963no
anaerobicyes69.353no
halophileyes86.583no
spore-formingno95.766no
glucose-utilyes89.649no
thermophileno98.331no
flagellatedno98.509no
aerobicno98.625yes
motileno96.424no
glucose-fermentyes90.983no

External links

@ref: 8503

culture collection no.: DSM 20075, ATCC 15009, CCUG 30139, IMET 10709, LMG 6413, CCTM 3036, JCM 1120, BCRC 12936, CCM 7193, CECT 4305, CIP 103146, IFO 15019, KCTC 3545, LMG 13555, NBRC 15019, NCFB 2712, NCIMB 11971, NRIC 1545, NRRL B-4526, NCDO 2712

straininfo link

  • @ref: 75944
  • straininfo: 9755

literature

Pubmed-IDtitleauthorsjournalDOIyearmeshtopictopic2
7764382Mapping of three plasmids from Lactobacillus helveticus ATCC 15009.Fortina MG, Parini C, Rossi P, Manachini PLLett Appl Microbiol10.1111/j.1472-765x.1993.tb01473.x1993Lactobacillus/*genetics, Plasmids/*genetics, Restriction Mapping
8979334Isolation, purification, and amino acid sequence of lactobin A, one of the two bacteriocins produced by Lactobacillus amylovorus LMG P-13139.Contreras BG, De Vuyst L, Devreese B, Busanyova K, Raymaeckers J, Bosman F, Sablon E, Vandamme EJAppl Environ Microbiol10.1128/aem.63.1.13-20.19971997Alcoholic Beverages/microbiology, Amino Acid Sequence, Bacterial Proteins/biosynthesis/*genetics/*isolation & purification, Bacteriocins/biosynthesis/*genetics/*isolation & purification, Fermentation, Lactobacillus/*genetics/*metabolism, Molecular Sequence Data, Molecular Weight, Sequence Homology, Amino AcidEnzymologyPhylogeny
10545264Complete sequence of plasmid pLH1 from lactobacillus helveticus ATCC15009: analysis reveals the presence of regions homologous to other native plasmids from the host strain.Thompson JK, Foley S, McConville KJ, Nicholson C, Collins MA, Pridmore RDPlasmid10.1006/plas.1999.14281999Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Bacterial/analysis, Lactobacillus/chemistry/*genetics, Molecular Sequence Data, Plasmids/chemistry/*genetics, Recombinases, Sequence Homology, Amino Acid, Transposases/geneticsEnzymologyGenetics
11097908Nutritional requirements and nitrogen-dependent regulation of proteinase activity of Lactobacillus helveticus CRL 1062.Hebert EM, Raya RR, De Giori GSAppl Environ Microbiol10.1128/AEM.66.12.5316-5321.20002000Aminopeptidases/metabolism, Caseins/metabolism, Culture Media/chemistry, Endopeptidases/*metabolism, Hydrolysis, Lactobacillus/growth & development/*metabolism, Nitrogen/*metabolism, Serine Endopeptidases/metabolismMetabolismCultivation
16478513Plasmids from Lactobacillus helveticus: distribution and diversity among natural isolates.Ricci G, Borgo F, Fortina MGLett Appl Microbiol10.1111/j.1472-765X.2005.01847.x2006Cheese/*microbiology, *Food Microbiology, Genetic Variation, Italy, Lactobacillus helveticus/*genetics, Molecular Weight, Plasmids/chemistry/*geneticsBiotechnology
16549380Induction of systemic and mucosal immune response and decrease in Streptococcus pneumoniae colonization by nasal inoculation of mice with recombinant lactic acid bacteria expressing pneumococcal surface antigen A.Oliveira ML, Areas AP, Campos IB, Monedero V, Perez-Martinez G, Miyaji EN, Leite LC, Aires KA, Lee Ho PMicrobes Infect10.1016/j.micinf.2005.10.0202006Adhesins, Bacterial/biosynthesis/genetics/*immunology, Administration, Intranasal, Amino Acid Sequence, Animals, Antibodies, Bacterial/*analysis/blood, Bacterial Adhesion/genetics/*immunology, Bacterial Proteins/genetics, Female, Immunoglobulin A/analysis, Immunoglobulin G/blood, Lactobacillus/genetics/metabolism, Lipoproteins/biosynthesis/genetics/*immunology, Mice, Mice, Inbred C57BL, Molecular Sequence Data, Pneumococcal Infections/*immunology/*prevention & control, Pneumococcal Vaccines/*administration & dosage, Protein Sorting Signals/genetics, Respiratory Mucosa/*immunology, Saliva/immunology, Species Specificity, Streptococcus pneumoniae/*immunology, *Vaccination, Vaccines, DNA/*administration & dosageGeneticsPhylogeny
18155517Lactic acid production by mixed cultures of Kluyveromyces marxianus, Lactobacillus delbrueckii ssp. bulgaricus and Lactobacillus helveticus.Plessas S, Bosnea L, Psarianos C, Koutinas AA, Marchant R, Banat IMBioresour Technol10.1016/j.biortech.2007.10.0392007Cheese, Cooking, Culture Media, Ethanol/metabolism, Fermentation, Food Analysis, Food Handling/methods, Kluyveromyces/growth & development/*metabolism, Lactic Acid/analysis/*biosynthesis, Lactobacillus delbrueckii/growth & development/*metabolism, Lactobacillus helveticus/growth & development/*metabolismMetabolismCultivation
24242242Lactobacillus helveticus MIMLh5-specific antibodies for detection of S-layer protein in Grana Padano protected-designation-of-origin cheese.Stuknyte M, Brockmann EC, Huovinen T, Guglielmetti S, Mora D, Taverniti V, Arioli S, De Noni I, Lamminmaki UAppl Environ Microbiol10.1128/AEM.03057-132013Antibodies, Monoclonal/*immunology, Bacteriophages/immunology, Blotting, Western, Cheese/*microbiology, Escherichia coli/genetics, Food Analysis/*methods, Humans, Lactobacillus helveticus/*immunology, Membrane Glycoproteins/*immunology/metabolism, Peptide Library, Recombinant Proteins/genetics/immunology, Single-Chain Antibodies/geneticsPathogenicityMetabolism
25557185Distinctive proteolytic activity of cell envelope proteinase of Lactobacillus helveticus isolated from airag, a traditional Mongolian fermented mare's milk.Miyamoto M, Ueno HM, Watanabe M, Tatsuma Y, Seto Y, Miyamoto T, Nakajima HInt J Food Microbiol10.1016/j.ijfoodmicro.2014.12.0122014Amino Acid Sequence, Animals, Caseins/metabolism, Cell Membrane/*metabolism, Dairy Products/*microbiology, Endopeptidases/chemistry/genetics/*metabolism, Fermentation, Horses, Lactobacillus helveticus/*enzymology/*isolation & purification, Milk/*microbiology, Mongolia, Proteolysis, Sequence Homology, Amino AcidMetabolismEnzymology
27435541Functional Probiotic Characterization and In Vivo Cholesterol-Lowering Activity of Lactobacillus helveticus Isolated from Fermented Cow Milk.Damodharan K, Palaniyandi SA, Yang SH, Suh JWJ Microbiol Biotechnol10.4014/jmb.1603.030052016Animals, Body Weight/drug effects, Caco-2 Cells, Cattle, Cholesterol/analysis/*metabolism, Cultured Milk Products/*microbiology, Eating/drug effects, Humans, Lactobacillus helveticus/*metabolism, Liver/chemistry/drug effects/metabolism, Mice, Mice, Inbred ICR, Probiotics/*pharmacologyPathogenicityMetabolism
27792948Isolation and characterization of Lactobacillus helveticus DSM 20075 variants with improved autolytic capacity.Spus M, Liu H, Wels M, Abee T, Smid EJInt J Food Microbiol10.1016/j.ijfoodmicro.2016.10.0202016*Bacteriolysis, Cheese/analysis/*microbiology, Fermentation, Hot Temperature, Lactobacillus helveticus/*cytology/genetics/isolation & purification/metabolism, Milk Proteins/metabolismPhylogenyStress
30813223beta-Galactosidase from Lactobacillus helveticus DSM 20075: Biochemical Characterization and Recombinant Expression for Applications in Dairy Industry.Kittibunchakul S, Pham ML, Tran AM, Nguyen THInt J Mol Sci10.3390/ijms200409472019Biocatalysis, *Dairying, Enzyme Stability, Escherichia coli/metabolism, Hydrogen-Ion Concentration, Hydrolysis, Kinetics, Lactobacillus helveticus/*enzymology/growth & development, Recombinant Proteins/*metabolism, Temperature, Time Factors, Trisaccharides/metabolismEnzymologyMetabolism
31293536Genomic Comparison of Lactobacillus helveticus Strains Highlights Probiotic Potential.Fontana A, Falasconi I, Molinari P, Treu L, Basile A, Vezzi A, Campanaro S, Morelli LFront Microbiol10.3389/fmicb.2019.013802019
32196339Structural Comparison of Different Galacto-oligosaccharide Mixtures Formed by beta-Galactosidases from Lactic Acid Bacteria and Bifidobacteria.Kittibunchakul S, van Leeuwen SS, Dijkhuizen L, Haltrich D, Nguyen THJ Agric Food Chem10.1021/acs.jafc.9b081562020Bacterial Proteins/genetics/*metabolism, Bifidobacterium/chemistry/genetics/*metabolism, Carbohydrate Conformation, Lactobacillales/chemistry/genetics/*metabolism, Lactobacillus helveticus/chemistry/*enzymology/genetics, Lactose/metabolism, Oligosaccharides/*chemistry/metabolism, beta-Galactosidase/genetics/*metabolismMetabolismEnzymology
35060080Development of Thermotolerant Lactobacilli Cultures with Improved Probiotic Properties Using Adaptive Laboratory Evolution Method.Bommasamudram J, Kumar P, Kapur S, Sharma D, Devappa SProbiotics Antimicrob Proteins10.1007/s12602-021-09892-32022

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8503Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20075)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20075
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37298Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14911
49933Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 30139)https://www.ccug.se/strain?id=30139
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
75944Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID9755.1StrainInfo: A central database for resolving microbial strain identifiers
119869Curators of the CIPCollection of Institut Pasteur (CIP 103146)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103146