Strain identifier

BacDive ID: 6461

Type strain: Yes

Species: Fructilactobacillus fructivorans

Strain history: CIP <- 1987, DSMZ <- R.H. Vaughn <- C.S. Pederson <- K.O. Stetter <- Fujikii

NCBI tax ID(s): 1614 (species)

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General

@ref: 8474

BacDive-ID: 6461

DSM-Number: 20203

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic

description: Fructilactobacillus fructivorans CCUG 32260 is an anaerobe, mesophilic bacterium of the family Lactobacillaceae.

NCBI tax id

  • NCBI tax id: 1614
  • Matching level: species

strain history

@refhistory
8474<- ATCC <- R.H. Vaughn <- C.S. Pederson
67770ATCC 8288 <-- R. H. Vaughn <-- C. S. Pederson.
119467CIP <- 1987, DSMZ <- R.H. Vaughn <- C.S. Pederson <- K.O. Stetter <- Fujikii

doi: 10.13145/bacdive6461.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Fructilactobacillus
  • species: Fructilactobacillus fructivorans
  • full scientific name: Fructilactobacillus fructivorans (Charlton et al. 1934) Zheng et al. 2020

  • synonyms

    @refsynonym
    20215Lactobacillus trichodes
    20215Lactobacillus heterohiochii
    20215Lactobacillus homohiochii
    20215Lactobacillus fructivorans

@ref: 8474

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Fructilactobacillus

species: Fructilactobacillus fructivorans

full scientific name: Fructilactobacillus fructivorans (Charlton et al. 1934) Zheng et al. 2020

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no94.936
69480100positive
119467yespositiverod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8474MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
37107MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
119467CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
8474positivegrowth30mesophilic
37107positivegrowth25mesophilic
50936positivegrowth30mesophilic
67770positivegrowth37mesophilic
119467positivegrowth22-37
119467nogrowth10psychrophilic
119467nogrowth15psychrophilic
119467nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
50936anaerobe
119467facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.998

murein

  • @ref: 8474
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
119467nitrate-reduction17632
119467nitrite-reduction16301
119467nitrate+respiration17632

antibiotic resistance

  • @ref: 119467
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite tests

  • @ref: 119467
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
119467oxidase-
119467alcohol dehydrogenase-1.1.1.1
119467catalase-1.11.1.6
119467lysine decarboxylase-4.1.1.18
119467ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119467-----+++--++---+----

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8474-----------++-------------------------------------
8474-----------++----------------------------------+--
119467----+-----++---------------+------------------+--

Isolation, sampling and environmental information

taxonmaps

  • @ref: 69479
  • File name: preview.99_3338.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_1742;97_2083;98_2559;99_3338&stattab=map
  • Last taxonomy: Fructilactobacillus fructivorans subclade
  • 16S sequence: LC064889
  • Sequence Identity:
  • Total samples: 639
  • soil counts: 41
  • aquatic counts: 96
  • animal counts: 438
  • plant counts: 64

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
84741Risk group (German classification)
1194671Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus fructivorans ATCC 8288 16S ribosomal RNA gene, partial sequenceAF429507473ena941770
20218Lactobacillus fructivorans strain ATCC 8288 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF429594545ena941770
20218Lactobacillus fructivorans strain ATCC 8288 16S-23S ribosomal RNA intergenic spacer and 23S ribosomal RNA gene, partial sequenceEU161579828ena941770
20218Lactobacillus fructivorans 16S ribosomal RNAM588181520ena1614
20218L.fructivorans (DSM 20203 T) 16S rRNA geneX763301570ena941770
20218Lactobacillus fructivorans gene for 16S rRNA, partial sequence, strain: JCM 1117AB289115643ena941770
20218Lactobacillus fructivorans gene for 16S ribosomal RNA, partial sequenceD31690176ena941770
20218Lactobacillus fructivorans gene for 16S rRNA, partial sequence, strain: NBRC 13954AB6805321501ena1614
67770Lactobacillus fructivorans gene for 16S ribosomal RNA, partial sequence, strain: JCM 1117LC0648891478ena1614

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus fructivorans KCTC 3543941770.3wgspatric1614
66792Lactobacillus fructivorans KCTC 3543 = DSM 20203941770.4wgspatric1614
66792Lactobacillus fructivorans KCTC 3543 = DSM 20203941770.5wgspatric1614
66792Fructilactobacillus fructivorans W2, KCTC 3543651324061draftimg941770
66792Fructilactobacillus fructivorans DSM 202032901599293draftimg941770
66792Fructilactobacillus fructivorans DSM 202032660238229draftimg941770
67770Fructilactobacillus fructivorans KCTC 3543GCA_000185465scaffoldncbi1614
67770Fructilactobacillus fructivorans DSM 20203GCA_001434095contigncbi1614
67770Fructilactobacillus fructivorans KCTC 3543GCA_003368395contigncbi1614

GC content

  • @ref: 67770
  • GC-content: 38.9
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno96.244no
flagellatedno98.466no
gram-positiveyes95.154no
anaerobicno70.44yes
halophileyes93.126no
spore-formingno97.385no
glucose-utilyes86.336no
aerobicno95.085yes
thermophileno99.263yes
glucose-fermentyes82.178no

External links

@ref: 8474

culture collection no.: CCUG 32260, NCIMB 8039, JCM 1117, IFO 13954, DSM 20203, ATCC 8288, NCIB 8039, BCRC 14623, CECT 4785, CIP 103042, KCTC 3543, LMG 9201, NBRC 13954, NCFB 2167, NRIC 0973, NRRL B-1841, VTT E-91473, NCDO 2167

straininfo link

  • @ref: 75933
  • straininfo: 3295

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny17609153New insertion sequence in Lactobacillus fructivorans strains isolated from spoiled sake.Wada Y, Mizoguchi HJ Biosci Bioeng10.1263/jbb.103.3992007Base Sequence, DNA Transposable Elements/*genetics, DNA, Bacterial/*genetics, Lactobacillus/*classification/*genetics, Molecular Sequence Data, Sequence Homology, Nucleic Acid, Species Specificity, Wine/*microbiologyGenetics
Metabolism19000615Biofilm formation by lactic acid bacteria and resistance to environmental stress.Kubota H, Senda S, Nomura N, Tokuda H, Uchiyama HJ Biosci Bioeng10.1263/jbb.106.3812008Acetic Acid/chemistry, *Biofilms, Colony Count, Microbial, Environment, Ethanol/chemistry, Fermentation, Food Industry, Lactic Acid/*metabolism, Lactobacillus/*metabolism, Microscopy, Electron, Scanning, Onions/microbiology, Plankton, Temperature, Time FactorsStress
Genetics22461550Genome sequence of Lactobacillus fructivorans KCTC 3543.Nam SH, Choi SH, Kang A, Lee KS, Kim DW, Kim RN, Kim DS, Park HSJ Bacteriol10.1128/JB.00075-122012Bacterial Proteins/genetics/metabolism, Base Sequence, Food Microbiology, Gene Expression Regulation, Bacterial, *Genome, Bacterial, Lactobacillus/classification/*genetics, Molecular Sequence DataBiotechnology
Phylogeny31407135Lactobacillus jinshani sp. nov., isolated from solid-state vinegar culture of Zhenjiang aromatic vinegar.Yu Y, Li X, Zhang J, Chai LJ, Lu ZM, Xu ZHAntonie Van Leeuwenhoek10.1007/s10482-019-01316-12019*Acetic Acid, Amino Acids, Diamino/metabolism, Bacterial Typing Techniques, Base Composition/genetics, DNA, Bacterial/genetics, Genome, Bacterial/genetics, Lactobacillus/classification/*genetics, Phylogeny, RNA, Ribosomal, 16S/geneticsGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8474Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20203)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20203
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37107Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14795
50936Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 32260)https://www.ccug.se/strain?id=32260
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
75933Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3295.1StrainInfo: A central database for resolving microbial strain identifiers
119467Curators of the CIPCollection of Institut Pasteur (CIP 103042)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103042