Strain identifier

BacDive ID: 6460

Type strain: No

Species: Limosilactobacillus fermentum

Strain Designation: 36

Strain history: CIP <- 1953, ATCC <- H.P. Sarett: strain 36 <- W. Peterson

NCBI tax ID(s): 1613 (species)

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General

@ref: 8759

BacDive-ID: 6460

DSM-Number: 20391

keywords: 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped, human pathogen

description: Limosilactobacillus fermentum 36 is an anaerobe, mesophilic, Gram-positive human pathogen of the family Lactobacillaceae.

NCBI tax id

  • NCBI tax id: 1613
  • Matching level: species

strain history

@refhistory
8759<- Difco Lab. <- ATCC <- H.P. Sarett, 36 <- W.H. Peterson
67770ATCC 9338 <-- H. P. Sarett 36 <-- W. H. Peterson.
119313CIP <- 1953, ATCC <- H.P. Sarett: strain 36 <- W. Peterson

doi: 10.13145/bacdive6460.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Limosilactobacillus
  • species: Limosilactobacillus fermentum
  • full scientific name: Limosilactobacillus fermentum (Beijerinck 1901) Zheng et al. 2020

  • synonyms

    @refsynonym
    20215Lactobacillus cellobiosus
    20215Lactobacillus fermentum

@ref: 8759

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Limosilactobacillus

species: Limosilactobacillus fermentum

full scientific name: Limosilactobacillus fermentum (Beijerinck 1901) Zheng et al. 2020

strain designation: 36

type strain: no

Morphology

cell morphology

  • @ref: 119313
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8759MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
35173MEDIUM 41- for Lactobacillus, Leuconostoc, Weissella, Pediococcus, Sporolactobacillus inulinusyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp broth (55.000 g)
119313CIP Medium 41yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=41

culture temp

@refgrowthtypetemperaturerange
8759positivegrowth37mesophilic
35173positivegrowth37mesophilic
47414positivegrowth37mesophilic
67770positivegrowth37mesophilic
119313positivegrowth22-45
119313nogrowth10psychrophilic
119313nogrowth15psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
47414anaerobe
47414microaerophile
119313facultative anaerobe

murein

  • @ref: 8759
  • murein short key: A21.04
  • type: A4ß L-Orn-D-Asp

observation

  • @ref: 67770
  • observation: Assay of Thiamine and pyrithiamine

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose+builds acid from28053
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
119313nitrate-reduction17632
119313nitrite-reduction16301
119313nitrate+respiration17632
68379nitrate-reduction17632
68379esculin-hydrolysis4853
68379urea+hydrolysis16199
68379gelatin+hydrolysis5291
68379D-ribose+fermentation16988
68379D-xylose+fermentation65327
68379D-mannitol-fermentation16899
68379maltose+fermentation17306
68379lactose+fermentation17716
68379sucrose-fermentation17992
68379glycogen-fermentation28087
68381arginine+hydrolysis29016
68381D-ribose+builds acid from16988
68381D-mannitol-builds acid from16899
68381sorbitol-builds acid from30911
68381lactose-builds acid from17716
68381trehalose-builds acid from27082
68381raffinose-builds acid from16634
68381sucrose-builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate-hydrolysis606565
68381glycogen-builds acid from28087
68381pullulan-builds acid from27941
68381maltose-builds acid from17306
68381melibiose+builds acid from28053
68381melezitose-builds acid from6731
68381methyl beta-D-glucopyranoside-builds acid from320055
68381D-tagatose-builds acid from16443
68381urea-hydrolysis16199

antibiotic resistance

  • @ref: 119313
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin+
11931315688acetoin-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase-3.2.1.21
68381arginine dihydrolase+3.5.3.6
68379catalase+1.11.1.6
68379gelatinase+
68379urease+3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15
119313oxidase-
119313alcohol dehydrogenase+1.1.1.1
119313catalase-1.11.1.6
119313lysine decarboxylase-4.1.1.18
119313ornithine decarboxylase-4.1.1.17

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlRIBXYLMANMALLACSACGLYGCAT
47414--++-++--++-++-++--+

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119313---+-+-+--++-+-+----

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8759-----++----+++--------------+-+----------------+--
8759-----++---+/-+++--------------+-+----------------+--
47414-----++---+++---------------+++----+-----------+--
119313----++---++++--------------++/-+----------------+--

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
47414+-+-+-+---------+-+-------+-----

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119313---------------------------------------------------------------------------------------------------

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
8759yes, in single cases1Risk group (German classification)
1193131Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus fermentum ATCC 9338 16S ribosomal RNA gene, partial sequenceAF429481515ena1613
20218Lactobacillus fermentum strain ATCC 9338 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF429568528ena1613

GC content

@refGC-content
875953.4
6777048.8

External links

@ref: 8759

culture collection no.: DSM 20391, ATCC 9338, CCM 91, NCDO 215, NCIB 6991, CCUG 21453, NCTC 6991, JCM 1560, BCRC 10360, CECT 285, CIP 53.163, IAM 1083, IAM 1148, IFO 3071, LMG 8896, NBRC 3071, NCFB 215, NCFB 8028, NCIMB 6991, VKM B-1311, VKM B-1566, VTT E-71033, NBIMCC 505, NCIMB 8028

straininfo link

  • @ref: 75932
  • straininfo: 3287

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Cultivation457268Serological studies on chemostat-grown cultures of Lactobacillus fermentum and Lactobacillus plantarum.Knox KW, Campbell LK, Broady KW, Wicken AJInfect Immun10.1128/iai.24.1.12-18.19791979Antibody Specificity, Antigens, Bacterial/*analysis, Antigens, Surface/analysis, Cell Wall/immunology, Culture Media, Lactobacillus/*classification/immunology, Polysaccharides, Bacterial/immunology, Ribitol, Serotyping, Teichoic Acids/immunologyPhylogeny
Biotechnology1907472Antibacterial activity of Lactobacillus sake isolated from dry fermented sausages.Sobrino OJ, Rodriguez JM, Moreira WL, Fernandez MF, Sanz B, Hernandez PEInt J Food Microbiol10.1016/0168-1605(91)90130-h1991Animals, Bacteria/*growth & development, Fermentation, *Food Microbiology, Lactobacillus/growth & development/*physiology, Listeria monocytogenes/growth & development, *Meat Products, Staphylococcus aureus/growth & development
Pathogenicity2632048Superoxide dismutase activity in some strains of lactobacilli: induction by manganese.Gonzalez SN, Apella MC, Romero N, Pesce de Ruiz Holgado AA, Oliver GChem Pharm Bull (Tokyo)10.1248/cpb.37.30261989Enzyme Induction/drug effects, Lactobacillus/*enzymology, Manganese/*pharmacology, Superoxide Dismutase/*biosynthesisEnzymology
Metabolism29633638Lipoteichoic acids are embedded in cell walls during logarithmic phase, but exposed on membrane vesicles in Lactobacillus gasseri JCM 1131(T).Shiraishi T, Yokota S, Sato Y, Ito T, Fukiya S, Yamamoto S, Sato T, Yokota ABenef Microbes10.3920/BM2017.01242018Antibodies, Monoclonal/metabolism, Antigens, Surface/*metabolism, Cell Membrane/chemistry/*metabolism/ultrastructure, Cell Wall/chemistry/*metabolism, Lactobacillus fermentum, Lactobacillus gasseri/chemistry/growth & development/*physiology/ultrastructure, Lactobacillus rhamnosus, Lipopolysaccharides/*metabolism, Probiotics, Protein Binding, Teichoic Acids/*metabolism
33546183Effects of Lacto-Fermented Agricultural By-Products as a Natural Disinfectant against Post-Harvest Diseases of Mango (Mangifera indica L.).Ranjith FH, Muhialdin BJ, Yusof NL, Mohammed NK, Miskandar MH, Hussin ASMPlants (Basel)10.3390/plants100202852021

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8759Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20391)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20391
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
35173Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17066
47414Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 21453)https://www.ccug.se/strain?id=21453
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
75932Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3287.1StrainInfo: A central database for resolving microbial strain identifiers
119313Curators of the CIPCollection of Institut Pasteur (CIP 53.163)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2053.163