Strain identifier

BacDive ID: 6430

Type strain: No

Species: Lacticaseibacillus rhamnosus

Strain Designation: 4R2127

Strain history: CIP <- 1957, NCIB <- 1950, NCTC <- 1948, M.B. Brown: strain Ld 5, Lactobacillus delbrueckii

NCBI tax ID(s): 47715 (species)

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General

@ref: 8656

BacDive-ID: 6430

DSM-Number: 20245

keywords: Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped

description: Lacticaseibacillus rhamnosus 4R2127 is an anaerobe, mesophilic, Gram-positive bacterium of the family Lactobacillaceae.

NCBI tax id

  • NCBI tax id: 47715
  • Matching level: species

strain history

@refhistory
8656<- ATCC <- Merck & Co., Inc., 4R2127 <- I.C. Gunsalus <- U. Wisconsin
67770ATCC 9595 <-- Merck & Co., Inc.; 4R2127 (Lactobacillus casei) <-- I. C. Gunsalus <-- U. Wisconsin.
122185CIP <- 1957, NCIB <- 1950, NCTC <- 1948, M.B. Brown: strain Ld 5, Lactobacillus delbrueckii

doi: 10.13145/bacdive6430.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lacticaseibacillus
  • species: Lacticaseibacillus rhamnosus
  • full scientific name: Lacticaseibacillus rhamnosus (Hansen 1968) Zheng et al. 2020

  • synonyms

    @refsynonym
    20215Lactobacillus casei subsp. rhamnosus
    20215Lactobacillus rhamnosus

@ref: 8656

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lacticaseibacillus

species: Lacticaseibacillus rhamnosus

full scientific name: Lacticaseibacillus rhamnosus (Hansen 1968) Zheng et al. 2020

strain designation: 4R2127

type strain: no

Morphology

cell morphology

  • @ref: 122185
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8656MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
40797MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
122185CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
8656positivegrowth37mesophilic
40797positivegrowth37mesophilic
52101positivegrowth30-37mesophilic
67770positivegrowth37mesophilic
122185positivegrowth15-45

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
52101anaerobe
52101microaerophile
122185facultative anaerobe

murein

  • @ref: 8656
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

observation

  • @ref: 67770
  • observation: Assay of Serine, aspartic and glutamic acids

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371inulin-builds acid from15443
68371melibiose-builds acid from28053
68371methyl alpha-D-mannoside-builds acid from43943
68371galactitol-builds acid from16813
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
122185nitrate-reduction17632
122185nitrite-reduction16301
122185nitrate+respiration17632
68379nitrate-reduction17632
68379esculin+hydrolysis4853
68379urea-hydrolysis16199
68379gelatin-hydrolysis5291
68379D-glucose+fermentation17634
68379D-ribose+fermentation16988
68379D-xylose-fermentation65327
68379D-mannitol+fermentation16899
68379maltose+fermentation17306
68379lactose+fermentation17716
68379sucrose+fermentation17992
68379glycogen-fermentation28087
68381arginine-hydrolysis29016
68381D-ribose+builds acid from16988
68381D-mannitol+builds acid from16899
68381sorbitol-builds acid from30911
68381lactose-builds acid from17716
68381trehalose-builds acid from27082
68381raffinose+builds acid from16634
68381sucrose+builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate+hydrolysis606565
68381glycogen-builds acid from28087
68381pullulan-builds acid from27941
68381maltose-builds acid from17306
68381melibiose-builds acid from28053
68381melezitose+builds acid from6731
68381methyl beta-D-glucopyranoside+builds acid from320055
68381D-tagatose+builds acid from16443
68381urea-hydrolysis16199

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin+
12218515688acetoin+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase+3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6
68379catalase-1.11.1.6
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15
122185oxidase-
122185alcohol dehydrogenase-1.1.1.1
122185catalase-1.11.1.6
122185lysine decarboxylase-4.1.1.18
122185ornithine decarboxylase-4.1.1.17

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlGLURIBXYLMANMALLACSACGLYGCAT
52101--++-++-+---++-++++--

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122185-+++-+++-+++-++-----

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
52101-----+----++++++-+++-+++++++++-++-+----++++-------
122185----+/-----+/-+/-+/-+/-+/-+/---+/-+/--+/-+/-+/-+/-+/-+/-+/-+/-+/--+/-+/--+/-----+/-+/-+/-+/-----+/---

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
52101-+----++---++---+-+++-+----+++--

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
86562Risk group (German classification)
1221851Risk group (French classification)

Sequence information

GC content

@refGC-contentmethod
865645.8
6777045.8thermal denaturation, midpoint method (Tm)

External links

@ref: 8656

culture collection no.: DSM 20245, ATCC 11979, ATCC 9595, CCM 1828, NCDO 207, NCIB 7473, NCIB 8019, CCUG 34425, NCTC 7473, JCM 1561, BCRC 11673, BUCSAV 226, CECT 288, CIP 57.6, NCFB 207, NCIMB 7473, NCIMB 8019, VKM B-575

straininfo link

  • @ref: 75905
  • straininfo: 35081

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism18387182Characterization and site-directed mutagenesis of Wzb, an O-phosphatase from Lactobacillus rhamnosus.LaPointe G, Atlan D, Gilbert CBMC Biochem10.1186/1471-2091-9-102008Amino Acid Sequence, Bacterial Proteins/genetics/*metabolism, Catalysis/drug effects, Cobalt/pharmacology, Electrophoresis, Polyacrylamide Gel, Histidinol-Phosphatase/genetics/metabolism, Hydrogen-Ion Concentration, Lactobacillus/*enzymology/genetics, Molecular Sequence Data, Mutagenesis, Site-Directed/*methods, Phosphoric Monoester Hydrolases/antagonists & inhibitors/genetics/*metabolism, Recombinant Proteins/antagonists & inhibitors/metabolism, Sequence Homology, Amino Acid, Substrate Specificity, Temperature, Vanadates/pharmacologyEnzymology
Metabolism19702865Intermediate chains of exopolysaccharides from Lactobacillus rhamnosus RW-9595M increase IL-10 production by macrophages.Bleau C, Monges A, Rashidan K, Laverdure JP, Lacroix M, Van Calsteren MR, Millette M, Savard R, Lamontagne LJ Appl Microbiol10.1111/j.1365-2672.2009.04450.x2009Animals, Culture Media, Conditioned, Female, Immunomodulation, Interleukin-10/*biosynthesis, Interleukin-12/metabolism, Interleukin-6/metabolism, Lactobacillus rhamnosus/*chemistry, Macrophages/drug effects/immunology/*metabolism, Mice, Mice, Inbred C57BL, Polysaccharides, Bacterial/*pharmacology, Tumor Necrosis Factor-alpha/metabolismCultivation
Metabolism25885688A tyrosine phosphorylation switch controls the interaction between the transmembrane modulator protein Wzd and the tyrosine kinase Wze of Lactobacillus rhamnosus.Kang HJ, Gilbert C, Badeaux F, Atlan D, LaPointe GBMC Microbiol10.1186/s12866-015-0371-22015Bacterial Proteins/*metabolism, *Gene Expression Regulation, Bacterial, Lactobacillus rhamnosus/genetics/metabolism/*physiology, Phosphorylation, Protein Binding, Protein Interaction Mapping, *Protein Processing, Post-Translational, Protein-Tyrosine Kinases/*metabolism
Pathogenicity27008259Lactobacillus rhamnosus could inhibit Porphyromonas gingivalis derived CXCL8 attenuation.Mendi A, Kose S, Uckan D, Akca G, Yilmaz D, Aral L, Gultekin SE, Eroglu T, Kilic E, Uckan SJ Appl Oral Sci10.1590/1678-7757201501452016Bacterial Adhesion/immunology, Cells, Cultured, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Humans, Immunity, Innate, Interferon-gamma/analysis/immunology, Interleukin-10, Interleukin-8/*analysis/immunology, Lactobacillus rhamnosus/*physiology, Mesenchymal Stem Cells/*microbiology, Periodontitis/microbiology, Porphyromonas gingivalis/*immunology, Probiotics/*pharmacology, Statistics, Nonparametric, Toll-Like Receptor 4/analysis/immunology, Young Adult
Pathogenicity27727499Lactobacillus rhamnosus inhibits Candida albicans virulence factors in vitro and modulates immune system in Galleria mellonella.Ribeiro FC, de Barros PP, Rossoni RD, Junqueira JC, Jorge AOJ Appl Microbiol10.1111/jam.133242016Animals, *Antibiosis, Biofilms, Candida albicans/*genetics/physiology, Immune System/immunology, Lactobacillus rhamnosus/*physiology, Larva/immunology/microbiology, Moths/*immunology/microbiology, Virulence Factors/*genetics/metabolismMetabolism
28457464Alginate based nanocomposite for microencapsulation of probiotic: Effect of cellulose nanocrystal (CNC) and lecithin.Huq T, Fraschini C, Khan A, Riedl B, Bouchard J, Lacroix MCarbohydr Polym10.1016/j.carbpol.2017.03.0322017Alginates/*chemistry, Cellulose/*chemistry, Drug Compounding, Hexuronic Acids, Lecithins/*chemistry, *Nanocomposites, Nanoparticles, Probiotics/*administration & dosage
31547398Antifungal and Antivirulence Activity of Vaginal Lactobacillus Spp. Products against Candida Vaginal Isolates.Itapary Dos Santos C, Ramos Franca Y, Duarte Lima Campos C, Quaresma Bomfim MR, Oliveira Melo B, Assuncao Holanda R, Santos VL, Gomes Monteiro S, Buozzi Moffa E, Souza Monteiro A, Andrade Monteiro C, Monteiro-Neto VPathogens10.3390/pathogens80301502019
32850769Fermentation of Wheat Bran and Whey Permeate by Mono-Cultures of Lacticaseibacillus rhamnosus Strains and Co-culture With Yeast Enhances Bioactive Properties.Bertsch A, Roy D, LaPointe GFront Bioeng Biotechnol10.3389/fbioe.2020.009562020
Biotechnology33809284Evaluation of Growth, Viability, Lactic Acid Production and Anti-Infective Effects of Lacticaseibacillus rhamnosus ATCC 9595 in Bacuri Juice (Platonia insignis).Mendes YC, Mesquita GP, Costa GDE, Barbosa da Silva AC, Gouveia E, Silva MRC, Monteiro-Neto V, Miranda RCM, Nascimento da Silva LC, Zagmignan AFoods10.3390/foods100306032021

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8656Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20245)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20245
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
40797Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/9589
52101Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 34425)https://www.ccug.se/strain?id=34425
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
75905Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID35081.1StrainInfo: A central database for resolving microbial strain identifiers
122185Curators of the CIPCollection of Institut Pasteur (CIP 57.6)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2057.6