Strain identifier

BacDive ID: 6414

Type strain: No

Species: Lactobacillus amylovorus

Strain Designation: B-4542

Strain history: CIP <- 1995, JCM <- NRRL: strain B-4542 <- L.K. Nakamura <- G. Hrubant

NCBI tax ID(s): 1604 (species)

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General

@ref: 8899

BacDive-ID: 6414

DSM-Number: 20532

keywords: 16S sequence, Bacteria, anaerobe, Gram-positive, rod-shaped, lactate production

description: Lactobacillus amylovorus B-4542 is an anaerobe, Gram-positive, rod-shaped bacterium that produces lactate and was isolated from cattle waste-corn fermentation.

NCBI tax id

  • NCBI tax id: 1604
  • Matching level: species

strain history

@refhistory
8899<- NRRL <- L.K. Nakamura <- G.R. Hrubant
67770NRRL B-4542 <-- L. K. Nakamura <-- G. R. Hurbant.
116366CIP <- 1995, JCM <- NRRL: strain B-4542 <- L.K. Nakamura <- G. Hrubant

doi: 10.13145/bacdive6414.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactobacillus
  • species: Lactobacillus amylovorus
  • full scientific name: Lactobacillus amylovorus Nakamura 1981

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus sobrius

@ref: 8899

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus amylovorus

full scientific name: Lactobacillus amylovorus Nakamura 1981

strain designation: B-4542

type strain: no

Morphology

cell morphology

  • @ref: 116366
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8899MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
34050MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
116366CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperature
8899positivegrowth37
34050positivegrowth37
67770positivegrowth37
116366positivegrowth37-45
116366nogrowth15

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8899anaerobe
8899microaerophile
116366facultative anaerobe

compound production

  • @ref: 8899
  • compound: DL lactic acid

murein

  • @ref: 8899
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371esculin+builds acid from4853
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
116366nitrate-reduction17632
116366nitrite-reduction16301
116366nitrate+respiration17632
68379nitrate-reduction17632
68379esculin+hydrolysis4853
68379urea-hydrolysis16199
68379gelatin-hydrolysis5291
68379D-glucose+fermentation17634
68379D-ribose-fermentation16988
68379D-xylose-fermentation65327
68379D-mannitol+fermentation16899
68379maltose+fermentation17306
68379lactose-fermentation17716
68379sucrose+fermentation17992
68379glycogen-fermentation28087
68381arginine-hydrolysis29016
68381D-ribose-builds acid from16988
68381D-mannitol-builds acid from16899
68381sorbitol-builds acid from30911
68381lactose-builds acid from17716
68381trehalose+builds acid from27082
68381raffinose-builds acid from16634
68381sucrose-builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate-hydrolysis606565
68381glycogen-builds acid from28087
68381pullulan-builds acid from27941
68381maltose+builds acid from17306
68381melibiose-builds acid from28053
68381melezitose-builds acid from6731
68381methyl beta-D-glucopyranoside-builds acid from320055
68381D-tagatose-builds acid from16443
68381urea-hydrolysis16199

metabolite production

@refChebi-IDmetaboliteproduction
6777024996lactateyes
6838115688acetoinno

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
116366oxidase-
116366alcohol dehydrogenase-1.1.1.1
116366amylase+
116366catalase-1.11.1.6
116366lysine decarboxylase-4.1.1.18
116366ornithine decarboxylase-4.1.1.17

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    48683C12:01.112
    48683C14:08.414
    48683C16:02816
    48683C18:0918
    48683C16:1 ω7c2.615.819
    48683C18:1 ω9c23.817.769
    48683C18:2 ω6,9c/C18:0 ANTE25.217.724
    48683C19:0 CYCLO ω9c218.87
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlGLURIBXYLMANMALLACSACGLYG
48683-----+--+---+--++-+-

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116366-++-------++-+-++---

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8899----------++++----+---++-++++--++---++-+----------
48683----------++++----+---+++++++--++---++-+----------
116366---------++++----+/----+++/-++/-+++/--++---++-+----------

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
48683-+--------+------++------+------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116366---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample type
8899cattle waste-corn fermentation
48683Cattle waste-corn fermentation
67770Cattle waste-corn fermentation
116366Cattle waste-corn fermentation

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Solid waste
#Host#Mammals#Bovinae (Cow, Cattle)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
88991Risk group (German classification)
1163661Risk group (French classification)

Sequence information

16S sequences

  • @ref: 124043
  • description: Lactobacillus amylovorus gene for 16S rRNA, partial sequence, strain: JCM 1127.
  • accession: AB289032
  • length: 689
  • database: nuccore
  • NCBI tax ID: 1604

GC content

  • @ref: 8899
  • GC-content: 40.2

External links

@ref: 8899

culture collection no.: CCUG 27202, DSM 20532, ATCC 33622, NRRL B-4542, JCM 1127, CCM 4381, CIP 104571

straininfo link

  • @ref: 75889
  • straininfo: 7308

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Cultivation15765056Optimization of lactic acid production in SSF by Lactobacillus amylovorus NRRL B-4542 using Taguchi methodology.Nagarjun PA, Rao RS, Rajesham S, Rao LVJ Microbiol21402005Bacteriological Techniques, Culture Media, Fermentation, Hydrogen-Ion Concentration, Lactic Acid/*biosynthesis, Lactobacillus acidophilus/*metabolism, Starch/metabolism, TemperatureMetabolism
Biotechnology17225102Fermentative production of lactic acid from biomass: an overview on process developments and future perspectives.John RP, Nampoothiri KM, Pandey AAppl Microbiol Biotechnol10.1007/s00253-006-0779-62007*Biomass, Biotechnology/*methods, *Fermentation, Lactic Acid/*metabolism, Lactobacillus/growth & development/*metabolismMetabolism

Reference

@idauthorscataloguedoi/urltitle
8899Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20532)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20532
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
34050Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16494
48683Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 27202)https://www.ccug.se/strain?id=27202
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
75889Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID7308.1StrainInfo: A central database for resolving microbial strain identifiers
116366Curators of the CIPCollection of Institut Pasteur (CIP 104571)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104571
124043Dr. Isabel Schober, Dr. Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy