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Strain identifier

BacDive ID: 64

Type strain: Yes

Species: Stella humosa

Strain history: <- VKM

NCBI tax ID(s): 94 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 2352

BacDive-ID: 64

DSM-Number: 5900

keywords: genome sequence, 16S sequence, Bacteria, chemoorganotroph, mesophilic, Gram-negative, star-shaped, colony-forming

description: Stella humosa DSM 5900 is a chemoorganotroph, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from soil.

NCBI tax id

  • NCBI tax id: 94
  • Matching level: species

strain history: <- VKM

doi: 10.13145/bacdive64.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Stellaceae
  • genus: Stella
  • species: Stella humosa
  • full scientific name: Stella humosa Vasilyeva 1985

@ref: 2352

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Stellaceae

genus: Stella

species: Stella humosa

full scientific name: Stella humosa Vasilyeva 1985

type strain: yes

Morphology

cell morphology

  • @ref: 23248
  • gram stain: negative
  • cell length: 0.7-3.0 µm
  • cell shape: star-shaped
  • motility: no

colony morphology

  • @ref: 23248
  • colony size: 2.5 mm
  • colony color: greyish white
  • colony shape: circular
  • incubation period: 14 days

Culture and growth conditions

culture medium

  • @ref: 2352
  • name: ANCALOMICROBIUM MEDIUM (DSMZ Medium 603)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/603
  • composition: Name: ANCALOMICROBIUM MEDIUM (DSMZ Medium 603) Composition: Agar 15.0 g/l MgSO4 x 7 H2O 0.588119 g/l (NH4)2SO4 0.247525 g/l Glucose 0.247525 g/l Nitrilotriacetic acid 0.19802 g/l Na2HPO4 0.070297 g/l CaCl2 x 2 H2O 0.0660396 g/l ZnSO4 x 7 H2O 0.00108416 g/l FeSO4 x 7 H2O 0.00049505 g/l Na-EDTA 0.000247525 g/l (NH4)6Mo7O24 x 4 H2O 0.000183168 g/l MnSO4 x H2O 0.000152475 g/l Calcium pantothenate 4.95049e-05 g/l Nicotinamide 4.95049e-05 g/l Riboflavin 4.95049e-05 g/l Thiamine-HCl x 2 H2O 4.95049e-05 g/l CuSO4 x 5 H2O 3.88119e-05 g/l Co(NO3)2 x 6 H2O 2.45545e-05 g/l Biotin 1.9802e-05 g/l Folic acid 1.9802e-05 g/l Na2B4O7 x 10 H2O 1.75248e-05 g/l Vitamin B12 9.90099e-07 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
2352positivegrowth28-30mesophilic
23248positiveoptimum28-30mesophilic

culture pH

  • @ref: 23248
  • ability: positive
  • type: growth
  • pH: 7

Physiology and metabolism

nutrition type

  • @ref: 23248
  • type: chemoorganotroph

spore formation

  • @ref: 23248
  • spore formation: no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
23248309162-oxoglutarate-growth
2324817968butyrate-growth
2324815570D-alanine-growth
2324815740formate-growth
2324829806fumarate-growth
2324824265gluconate-growth
2324816977L-alanine-growth
2324816467L-arginine-growth
2324817196L-asparagine-growth
2324829991L-aspartate-growth
2324818050L-glutamine-growth
2324815971L-histidine-growth
2324817115L-serine-growth
2324826986threonine-growth
2324831011valerate-growth
2324862968cellulose-hydrolysis
232485291gelatin-hydrolysis
2324828017starch-hydrolysis
2324830089acetate+growth
2324816947citrate+growth
2324817561L-cysteine+growth
2324816283L-cystine+growth
2324829985L-glutamate+growth
2324818019L-lysine+growth
2324824996lactate+growth
2324825115malate+growth
2324815361pyruvate+growth
2324830031succinate+growth

antibiotic resistance

@refChEBImetaboliteis antibioticis intermediateintermediate conc.is sensitivesensitivity conc.
232487935paromomycin sulfateyesyes2-10 µg/mL
2324817334penicillinyesyes8 µg/mL
232487507neomycinyesyes0.5-1 µg/mL

enzymes

@refvalueactivityec
23248catalase+1.11.1.6
23248cytochrome oxidase+1.9.3.1

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
2352soilKrasnodarRussiaRUSEurope
23248Virgin chernozem

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_141379.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_634;96_20993;97_26151;98_33233;99_141379&stattab=map
  • Last taxonomy: Stella humosa
  • 16S sequence: AJ535710
  • Sequence Identity:
  • Total samples: 81
  • soil counts: 12
  • aquatic counts: 46
  • animal counts: 15
  • plant counts: 8

Safety information

risk assessment

  • @ref: 2352
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Stella humosa 16S rRNA gene, strain DSM5900
  • accession: AJ535710
  • length: 1447
  • database: ena
  • NCBI tax ID: 94

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Stella humosa DSM 5900GCA_003751345contigncbi94
66792Stella humosa ATCC 43930GCA_006738645completencbi94
66792Stella humosa strain ATCC 4393094.5completepatric94
66792Stella humosa strain DSM 590094.4wgspatric94
66792Stella humosa DSM 59002788499841draftimg94

GC content

  • @ref: 23248
  • GC-content: 72.70
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 2352

culture collection no.: DSM 5900, ATCC 43930, AUCM B-1137, BKM B-1137, DSM 3698, VKM B-1137

straininfo link

@refpassport
20218http://www.straininfo.net/strains/159512
20218http://www.straininfo.net/strains/113335
20218http://www.straininfo.net/strains/303871
20218http://www.straininfo.net/strains/159513
20218http://www.straininfo.net/strains/113336

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15143003Phylogenetic relationships of the genera Stella, Labrys and Angulomicrobium within the 'Alphaproteobacteria' and description of Angulomicrobium amanitiforme sp. nov.Fritz I, Strompl C, Abraham WRInt J Syst Evol Microbiol10.1099/ijs.0.02746-02004Acetobacteraceae/*classification/genetics/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Genes, Bacterial, Hyphomicrobiaceae/*classification/genetics/metabolism, Lipids/analysis, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/geneticsMetabolism
Genetics31395645Complete Genome Sequences of Three Star-Shaped Bacteria, Stella humosa, Stella vacuolata, and Stella Species ATCC 35155.Shibai A, Maeda T, Kawada M, Kotani H, Sakata N, Furusawa CMicrobiol Resour Announc10.1128/MRA.00719-192019

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2352Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5900)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5900
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23248L. V. VASILYEVA10.1099/00207713-35-4-518Stella, a New Genus of Soil Prosthecobacteria, with Proposals for Stella humosa sp. nov. and Stella vacuolata sp. nov.IJSEM 35: 518-521 1985
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta