Strain identifier

BacDive ID: 6397

Type strain: Yes

Species: Pediococcus stilesii

Strain Designation: FAIR-E-180

Culture col. no.: DSM 18001, CCUG 51290, LMG 23082, CIP 109142, JCM 30772, BCRC 17601, FAIR-E 180, NCIMB 14315

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 7228

BacDive-ID: 6397

DSM-Number: 18001

keywords: coccus-shaped, microaerophile, mesophilic, 16S sequence, Bacteria, Gram-positive, genome sequence

description: Pediococcus stilesii FAIR-E-180 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from white maize grains.

strain history

doi: 10.13145/bacdive6397.20211221.6

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/firmicutes
  • domain: Bacteria
  • phylum: Firmicutes
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Pediococcus
  • species: Pediococcus stilesii
  • full scientific name: Pediococcus stilesii Franz et al. 2006

@ref: 7228

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Pediococcus

species: Pediococcus stilesii

full scientific name: Pediococcus stilesii Franz et al. 2006

strain designation: FAIR-E-180

type strain: yes

Morphology

cell morphology

  • @ref: 31637
  • gram stain: positive
  • cell width: 0.6-1.2 µm
  • cell shape: coccus-shaped
  • motility: no

pigmentation

  • @ref: 31637
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7228MRS MEDIUM (DSMZ Medium 11)yeshttps://bacmedia.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
40127MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and NeisseriayesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml)

culture temp

@refgrowthtypetemperaturerange
7228positivegrowth30mesophilic
31637positivegrowth35-48
31637positiveoptimum35-48
40127positivegrowth37mesophilic
59133positivegrowth30mesophilic
59133positivegrowth37mesophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepH
31637positivegrowth4.5-8
31637positiveoptimum4.5-8

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
7228microaerophile
31637aerobe
59133anaerobe
59133microaerophile

spore formation

  • @ref: 31637
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
31637NaClpositivegrowth10 %
31637NaClpositiveoptimum10 %

observation

  • @ref: 31637
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3163717057cellobiose+carbon source
3163728757fructose+carbon source
3163728260galactose+carbon source
3163717234glucose+carbon source
3163717306maltose+carbon source
3163737684mannose+carbon source
31637506227N-acetylglucosamine+carbon source
3163733942ribose+carbon source
3163717814salicin+carbon source

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
7228white maize grainsLagosNigeriaNGAAfrica
59133Maize,white graines after 3 days of steepingLagosNigeriaNGAAfrica
67770White maize grains

isolation source categories

  • Cat1: #Host
  • Cat2: #Plants
  • Cat3: #Herbaceous plants (Grass,Crops)

Safety information

risk assessment

  • @ref: 7228
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 7228
  • description: Pediococcus stilesii 16S rRNA gene, type strain LMG 23082T
  • accession: AJ973157
  • length: 1529
  • database: ena
  • NCBI tax ID: 331679

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pediococcus stilesii DSM 18001GCA_001437075contigncbi331679
66792Pediococcus stilesii FAM 18815GCA_005864415scaffoldncbi331679
66792Pediococcus stilesii strain DSM 18001331679.3wgspatric331679
66792Pediococcus stilesii strain FAM 18815331679.4wgspatric331679
66792Pediococcus stilesii DSM 180012667527983draftimg331679
67770Pediococcus stilesii strain DSM 18001, whole genome shotgun sequencing projectJQBX00000000nuccore331679

GC content

@refGC-contentmethod
722838
6777038thermal denaturation, midpoint method (Tm)

External links

@ref: 7228

culture collection no.: DSM 18001, CCUG 51290, LMG 23082, CIP 109142, JCM 30772, BCRC 17601, FAIR-E 180, NCIMB 14315

straininfo link

@refpassport
20218http://www.straininfo.net/strains/677474
20218http://www.straininfo.net/strains/675133
20218http://www.straininfo.net/strains/675134

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
7228Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18001)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18001
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
2792010.1099/ijs.0.63944-016449434
31637Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604127920
40127Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6851
59133Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 51290)https://www.ccug.se/strain?id=51290
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/