Strain identifier
BacDive ID: 6378
Type strain:
Species: Lachnoanaerobaculum orale
Strain Designation: N1
Strain history: <- M. Hedberg, Umea University, Institution of Clinical Microbiology, Div. of Immunology; N1 <- M. Hedberg, Karolinska Institute, Dept. IMPI, Stockholm, Sweden
NCBI tax ID(s): 979627 (species)
General
@ref: 17695
BacDive-ID: 6378
DSM-Number: 24553
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-positive, rod-shaped
description: Lachnoanaerobaculum orale N1 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from saliva from a healthy young man.
NCBI tax id
- NCBI tax id: 979627
- Matching level: species
strain history
- @ref: 17695
- history: <- M. Hedberg, Umea University, Institution of Clinical Microbiology, Div. of Immunology; N1 <- M. Hedberg, Karolinska Institute, Dept. IMPI, Stockholm, Sweden
doi: 10.13145/bacdive6378.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Lachnospiraceae
- genus: Lachnoanaerobaculum
- species: Lachnoanaerobaculum orale
- full scientific name: Lachnoanaerobaculum orale Hedberg et al. 2012
@ref: 17695
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Lachnospiraceae
genus: Lachnoanaerobaculum
species: Lachnoanaerobaculum orale
full scientific name: Lachnoanaerobaculum orale Hedberg et al. 2012
strain designation: N1
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell shape | confidence |
---|---|---|---|
30331 | positive | rod-shaped | |
69480 | positive | 100 |
colony morphology
- @ref: 62276
- incubation period: 2 days
Culture and growth conditions
culture medium
- @ref: 17695
- name: PY + X MEDIUM (N2/CO2) (DSMZ Medium 104c)
- growth: yes
- link: https://mediadive.dsmz.de/medium/104c
- composition: Name: PY + X MEDIUM (N2/CO2) (DSMZ Medium 104c) Composition: Yeast extract 10.0 g/l D-Glucose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l Na2CO3 1.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
30331 | positive | growth | 37 | mesophilic |
17695 | positive | growth | 37 | mesophilic |
62276 | positive | growth | 37 | mesophilic |
culture pH
- @ref: 30331
- ability: positive
- type: optimum
- pH: 6.75
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance | confidence |
---|---|---|
17695 | anaerobe | |
30331 | anaerobe | |
62276 | anaerobe | |
69480 | anaerobe | 100 |
spore formation
@ref | spore formation | confidence |
---|---|---|
30331 | yes | |
69481 | yes | 100 |
69480 | no | 99.896 |
enzymes
- @ref: 30331
- value: alpha-galactosidase
- activity: +
- ec: 3.2.1.22
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | sampling date |
---|---|---|---|---|---|---|
17695 | saliva from a healthy young man | Stockholm | Sweden | SWE | Europe | |
62276 | Human saliva | Umeå | Sweden | SWE | Europe | 2010-09-01 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Infection | #Patient | |
#Host Body Product | #Oral cavity and Airways | #Saliva |
#Host | #Human | #Male |
Safety information
risk assessment
- @ref: 17695
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 17695
- description: Lachnoanaerobaculum orale strain N1 16S ribosomal RNA gene, partial sequence
- accession: HQ877677
- length: 1435
- database: ena
- NCBI tax ID: 979627
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Lachnoanaerobaculum orale DSM 24553 | GCA_003862485 | scaffold | ncbi | 979627 |
66792 | Lachnoanaerobaculum orale strain DSM 24553 | 979627.3 | wgs | patric | 979627 |
GC content
@ref | GC-content | method |
---|---|---|
17695 | 38 | high performance liquid chromatography (HPLC) |
30331 | 38 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 100 | no |
flagellated | no | 92.879 | no |
gram-positive | yes | 88.564 | no |
anaerobic | yes | 98.98 | yes |
aerobic | no | 98.548 | no |
halophile | no | 79.903 | no |
spore-forming | no | 56.904 | yes |
thermophile | no | 98.631 | no |
glucose-util | yes | 88.92 | no |
motile | no | 88.459 | no |
glucose-ferment | yes | 80.215 | no |
External links
@ref: 17695
culture collection no.: DSM 24553, CCUG 60305
straininfo link
- @ref: 75851
- straininfo: 403494
literature
- topic: Phylogeny
- Pubmed-ID: 22228654
- title: Lachnoanaerobaculum gen. nov., a new genus in the Lachnospiraceae: characterization of Lachnoanaerobaculum umeaense gen. nov., sp. nov., isolated from the human small intestine, and Lachnoanaerobaculum orale sp. nov., isolated from saliva, and reclassification of Eubacterium saburreum (Prevot 1966) Holdeman and Moore 1970 as Lachnoanaerobaculum saburreum comb. nov.
- authors: Hedberg ME, Moore ERB, Svensson-Stadler L, Horstedt P, Baranov V, Hernell O, Wai SN, Hammarstrom S, Hammarstrom ML
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.033613-0
- year: 2012
- mesh: Adult, Bacterial Typing Techniques, Celiac Disease/microbiology, Child, DNA, Bacterial/genetics, Eubacterium/*classification/genetics/isolation & purification, Fatty Acids/analysis, Female, Humans, Intestine, Small/*microbiology, Male, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Saliva/*microbiology, Sequence Analysis, DNA
- topic2: Pathogenicity
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
17695 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24553) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-24553 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
30331 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26672 | 28776041 | |
62276 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 60305) | https://www.ccug.se/strain?id=60305 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
75851 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID403494.1 | StrainInfo: A central database for resolving microbial strain identifiers |