Strain identifier

BacDive ID: 6378

Type strain: Yes

Species: Lachnoanaerobaculum orale

Strain Designation: N1

Strain history: <- M. Hedberg, Umea University, Institution of Clinical Microbiology, Div. of Immunology; N1 <- M. Hedberg, Karolinska Institute, Dept. IMPI, Stockholm, Sweden

NCBI tax ID(s): 979627 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 17695

BacDive-ID: 6378

DSM-Number: 24553

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-positive, rod-shaped

description: Lachnoanaerobaculum orale N1 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from saliva from a healthy young man.

NCBI tax id

  • NCBI tax id: 979627
  • Matching level: species

strain history

  • @ref: 17695
  • history: <- M. Hedberg, Umea University, Institution of Clinical Microbiology, Div. of Immunology; N1 <- M. Hedberg, Karolinska Institute, Dept. IMPI, Stockholm, Sweden

doi: 10.13145/bacdive6378.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Lachnoanaerobaculum
  • species: Lachnoanaerobaculum orale
  • full scientific name: Lachnoanaerobaculum orale Hedberg et al. 2012

@ref: 17695

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Lachnospiraceae

genus: Lachnoanaerobaculum

species: Lachnoanaerobaculum orale

full scientific name: Lachnoanaerobaculum orale Hedberg et al. 2012

strain designation: N1

type strain: yes

Morphology

cell morphology

@refgram staincell shapeconfidence
30331positiverod-shaped
69480positive100

colony morphology

  • @ref: 62276
  • incubation period: 2 days

Culture and growth conditions

culture medium

  • @ref: 17695
  • name: PY + X MEDIUM (N2/CO2) (DSMZ Medium 104c)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/104c
  • composition: Name: PY + X MEDIUM (N2/CO2) (DSMZ Medium 104c) Composition: Yeast extract 10.0 g/l D-Glucose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l Na2CO3 1.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
30331positivegrowth37mesophilic
17695positivegrowth37mesophilic
62276positivegrowth37mesophilic

culture pH

  • @ref: 30331
  • ability: positive
  • type: optimum
  • pH: 6.75

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
17695anaerobe
30331anaerobe
62276anaerobe
69480anaerobe100

spore formation

@refspore formationconfidence
30331yes
69481yes100
69480no99.896

enzymes

  • @ref: 30331
  • value: alpha-galactosidase
  • activity: +
  • ec: 3.2.1.22

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
17695saliva from a healthy young manStockholmSwedenSWEEurope
62276Human salivaUmeåSwedenSWEEurope2010-09-01

isolation source categories

Cat1Cat2Cat3
#Infection#Patient
#Host Body Product#Oral cavity and Airways#Saliva
#Host#Human#Male

Safety information

risk assessment

  • @ref: 17695
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 17695
  • description: Lachnoanaerobaculum orale strain N1 16S ribosomal RNA gene, partial sequence
  • accession: HQ877677
  • length: 1435
  • database: ena
  • NCBI tax ID: 979627

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lachnoanaerobaculum orale DSM 24553GCA_003862485scaffoldncbi979627
66792Lachnoanaerobaculum orale strain DSM 24553979627.3wgspatric979627

GC content

@refGC-contentmethod
1769538high performance liquid chromatography (HPLC)
3033138

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
flagellatedno92.879no
gram-positiveyes88.564no
anaerobicyes98.98yes
aerobicno98.548no
halophileno79.903no
spore-formingno56.904yes
thermophileno98.631no
glucose-utilyes88.92no
motileno88.459no
glucose-fermentyes80.215no

External links

@ref: 17695

culture collection no.: DSM 24553, CCUG 60305

straininfo link

  • @ref: 75851
  • straininfo: 403494

literature

  • topic: Phylogeny
  • Pubmed-ID: 22228654
  • title: Lachnoanaerobaculum gen. nov., a new genus in the Lachnospiraceae: characterization of Lachnoanaerobaculum umeaense gen. nov., sp. nov., isolated from the human small intestine, and Lachnoanaerobaculum orale sp. nov., isolated from saliva, and reclassification of Eubacterium saburreum (Prevot 1966) Holdeman and Moore 1970 as Lachnoanaerobaculum saburreum comb. nov.
  • authors: Hedberg ME, Moore ERB, Svensson-Stadler L, Horstedt P, Baranov V, Hernell O, Wai SN, Hammarstrom S, Hammarstrom ML
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.033613-0
  • year: 2012
  • mesh: Adult, Bacterial Typing Techniques, Celiac Disease/microbiology, Child, DNA, Bacterial/genetics, Eubacterium/*classification/genetics/isolation & purification, Fatty Acids/analysis, Female, Humans, Intestine, Small/*microbiology, Male, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Saliva/*microbiology, Sequence Analysis, DNA
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
17695Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24553)https://www.dsmz.de/collection/catalogue/details/culture/DSM-24553
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30331Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2667228776041
62276Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 60305)https://www.ccug.se/strain?id=60305
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
75851Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID403494.1StrainInfo: A central database for resolving microbial strain identifiers