Strain identifier

BacDive ID: 6368

Type strain: Yes

Species: Roseburia faecis

Strain Designation: M72/1

Strain history: DSM 16840 <-- S. Duncan M72/1.

NCBI tax ID(s): 301302 (species)

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General

@ref: 6626

BacDive-ID: 6368

DSM-Number: 16840

keywords: 16S sequence, Bacteria, anaerobe, mesophilic

description: Roseburia faecis M72/1 is an anaerobe, mesophilic bacterium that was isolated from human faecal sample.

NCBI tax id

  • NCBI tax id: 301302
  • Matching level: species

strain history

@refhistory
6626<- S. Duncan; M72/1
67770DSM 16840 <-- S. Duncan M72/1.

doi: 10.13145/bacdive6368.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Roseburia
  • species: Roseburia faecis
  • full scientific name: Roseburia faecis Duncan et al. 2006

  • synonyms

    • @ref: 20215
    • synonym: Agathobacter faecis

@ref: 6626

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Lachnospiraceae

genus: Roseburia

species: Roseburia faecis

full scientific name: Roseburia faecis Duncan et al. 2006

strain designation: M72/1

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 6626
  • name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/58
  • composition: Name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58) Composition: Glucose 10.0 g/l Casein peptone 10.0 g/l Bacto Soytone 5.0 g/l Meat extract 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l MnSO4 x H2O 0.05 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Tween 80 Resazurin Distilled water

culture temp

@refgrowthtypetemperaturerange
6626positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 6626
  • oxygen tolerance: anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
6626--++-++/-----------+-----------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
6626human faecal sampleScotland, AberdeenUnited KingdomGBREurope
67770Human feces in AberdeenScotlandUnited KingdomGBREurope

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Gastrointestinal tract#Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_6780.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_16;96_2600;97_3925;98_4998;99_6780&stattab=map
  • Last taxonomy: Roseburia faecis subclade
  • 16S sequence: AY305310
  • Sequence Identity:
  • Total samples: 46960
  • soil counts: 152
  • aquatic counts: 615
  • animal counts: 46157
  • plant counts: 36

Safety information

risk assessment

  • @ref: 6626
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
6626Roseburia faecis M72/1 16S ribosomal RNA gene, partial sequenceAY3053101456ena451638
67770Roseburia faecis gene for 16S ribosomal RNA, partial sequence, strain: JCM 17581AB6614331495ena301302

GC content

@refGC-contentmethod
662642
6777042high performance liquid chromatography (HPLC)

External links

@ref: 6626

culture collection no.: DSM 16840, CIP 109404, JCM 17581, NCIMB 14031

straininfo link

  • @ref: 75841
  • straininfo: 237169

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny17012576Proposal of Roseburia faecis sp. nov., Roseburia hominis sp. nov. and Roseburia inulinivorans sp. nov., based on isolates from human faeces.Duncan SH, Aminov RI, Scott KP, Louis P, Stanton TB, Flint HJInt J Syst Evol Microbiol10.1099/ijs.0.64098-02006Bacteria, Anaerobic/*classification/genetics/growth & development/isolation & purification, Base Composition, Carbohydrate Metabolism, DNA, Bacterial/analysis, Fatty Acids/metabolism, Feces/*microbiology, Humans, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAMetabolism
Metabolism19633122In vitro kinetics of prebiotic inulin-type fructan fermentation by butyrate-producing colon bacteria: implementation of online gas chromatography for quantitative analysis of carbon dioxide and hydrogen gas production.Falony G, Verschaeren A, De Bruycker F, De Preter V, Verbeke K, Leroy F, De Vuyst LAppl Environ Microbiol10.1128/AEM.00876-092009Butyrates/metabolism, Carbon Dioxide/*analysis, Chromatography, Gas/*methods, Colon/*microbiology, Fermentation, Fructans/*metabolism, Gram-Positive Bacteria/growth & development/isolation & purification/*metabolism, Hydrogen/*analysis, Lactic Acid/metabolismEnzymology
Pathogenicity23916720A representative of the dominant human colonic Firmicutes, Roseburia faecis M72/1, forms a novel bacteriocin-like substance.Hatziioanou D, Mayer MJ, Duncan SH, Flint HJ, Narbad AAnaerobe10.1016/j.anaerobe.2013.07.0062013Bacillus subtilis/*drug effects, Bacteriocins/chemistry/isolation & purification/*metabolism, Gram-Positive Bacteria/isolation & purification/*metabolism, Humans, Protein Stability, TemperatureEnzymology
Phylogeny35559790Pararoseburia lenta gen. nov., sp. nov. isolated from human faeces.Abdugheni R, Wang YJ, Li DH, Du MX, Liu C, Zhou N, Liu SJInt J Syst Evol Microbiol10.1099/ijsem.0.0053712022*Bacteria, Anaerobic, Bacterial Typing Techniques, Base Composition, Butyrates, DNA, Bacterial/genetics, *Fatty Acids/chemistry, Feces/microbiology, Humans, Phospholipids/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAPathogenicity

Reference

@idauthorscataloguedoi/urltitle
6626Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16840)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16840
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75841Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID237169.1StrainInfo: A central database for resolving microbial strain identifiers