Strain identifier

BacDive ID: 6340

Type strain: Yes

Species: Anaerostipes butyraticus

Strain Designation: 35-7

Strain history: DSM 22094 <-- V. Eeckhaut 35-7.

NCBI tax ID(s): 645466 (species)

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General

@ref: 16136

BacDive-ID: 6340

DSM-Number: 22094

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, Gram-positive, rod-shaped

description: Anaerostipes butyraticus 35-7 is an anaerobe, spore-forming, thermophilic bacterium that was isolated from caecal content of a 4-week-old broiler chicken.

NCBI tax id

  • NCBI tax id: 645466
  • Matching level: species

strain history

@refhistory
16136<- V. Eeckhaut, Fac. Veterinary Medicine, Univ. Ghent, Merelbeke, Belgium; 35-7 <- V. Eeckhaut {2008}
67770DSM 22094 <-- V. Eeckhaut 35-7.

doi: 10.13145/bacdive6340.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Anaerostipes
  • species: Anaerostipes butyraticus
  • full scientific name: Anaerostipes butyraticus Eeckhaut et al. 2010

@ref: 16136

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Lachnospiraceae

genus: Anaerostipes

species: Anaerostipes butyraticus

full scientific name: Anaerostipes butyraticus Eeckhaut et al. 2010

strain designation: 35-7

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell shapemotilityconfidence
29535positive10 µmrod-shapedno
69480no94.576
69480positive100

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16136PY + X MEDIUM (DSMZ Medium 104b)yeshttps://mediadive.dsmz.de/medium/104bName: PY + X MEDIUM (DSMZ Medium 104b) Composition: Yeast extract 10.0 g/l D-Glucose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water
16136CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)yeshttps://mediadive.dsmz.de/medium/110Name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l D-Glucose 4.0 g/l Starch 1.0 g/l Maltose 1.0 g/l Cellobiose 1.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Sodium resazurin 0.0005 g/l Vitamin K1 NaOH Distilled water

culture temp

@refgrowthtypetemperaturerange
16136positivegrowth40thermophilic
29535positivegrowth35-47
29535positiveoptimum39mesophilic
67770positivegrowth37mesophilic

culture pH

@refabilitytypepH
29535positivegrowth5.5-8
29535positiveoptimum6

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
16136anaerobe
29535anaerobe
69480anaerobe99.999

spore formation

@refspore formationconfidence
29535yes
69480no98.552

compound production

  • @ref: 16136
  • compound: butyrate

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2953517057cellobiose+carbon source
2953517234glucose+carbon source
2953517306maltose+carbon source
2953529864mannitol+carbon source
2953537684mannose+carbon source
2953516634raffinose+carbon source
2953517814salicin+carbon source
2953530911sorbitol+carbon source
2953517992sucrose+carbon source
2953527082trehalose+carbon source

enzymes

  • @ref: 29535
  • value: acid phosphatase
  • activity: +
  • ec: 3.1.3.2

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
16136caecal content of a 4-week-old broiler chickenGhentBelgiumBELEurope
67770Cecal content of a 4-week-old broiler chicken in GhentBelgiumBELEurope

isolation source categories

Cat1Cat2Cat3
#Host#Birds#Chicken
#Host Body-Site#Gastrointestinal tract#Large intestine
#Host Body Product#Gastrointestinal tract#Caecal content

taxonmaps

  • @ref: 69479
  • File name: preview.99_149249.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_33;96_11980;97_14521;98_17941;99_149249&stattab=map
  • Last taxonomy: Anaerostipes butyraticus subclade
  • 16S sequence: FJ947528
  • Sequence Identity:
  • Total samples: 3467
  • soil counts: 109
  • aquatic counts: 39
  • animal counts: 3313
  • plant counts: 6

Safety information

risk assessment

  • @ref: 16136
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Anaerostipes butyraticus gene for 16S ribosomal RNA, partial sequence, strain: JCM 17466AB6161341495ena645466
16136Anaerostipes butyraticus strain 35-7 16S ribosomal RNA gene, partial sequenceFJ9475281381ena645466

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Anaerostipes butyraticus JCM 17466GCA_016586355contigncbi645466
66792Anaerostipes butyraticus strain JCM 17466645466.3wgspatric645466

GC content

@refGC-contentmethod
1613644high performance liquid chromatography (HPLC)
2953544

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes61no
gram-positiveyes93.751no
anaerobicyes99.493no
halophileno72.822no
spore-formingno53.536yes
glucose-utilyes92.915no
flagellatedno93.725yes
aerobicno99.058yes
thermophileno93.969no
motileno91.604yes
glucose-fermentyes67.692no

External links

@ref: 16136

culture collection no.: DSM 22094, JCM 17466, LMG 24724

straininfo link

  • @ref: 75814
  • straininfo: 370145

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19666785Anaerostipes butyraticus sp. nov., an anaerobic, butyrate-producing bacterium from Clostridium cluster XIVa isolated from broiler chicken caecal content, and emended description of the genus Anaerostipes.Eeckhaut V, Van Immerseel F, Pasmans F, De Brandt E, Haesebrouck F, Ducatelle R, Vandamme PInt J Syst Evol Microbiol10.1099/ijs.0.015289-02009Anaerobiosis, Animals, Bacterial Typing Techniques, Belgium, Butyrates/*metabolism, Cecum/*microbiology, Chickens/*microbiology, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Genotype, Gram-Positive Asporogenous Rods/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species SpecificityGenetics
Phylogeny34491487Description of Anaerostipes faecalis sp. nov., a new segmented filamentous bacterium isolated from swine faeces.Choi SH, Choi JY, Park JE, Kim JS, Kang SW, Lee J, Lee MK, Lee JS, Lee JH, Jung H, Hur TY, Kim HB, Lee JH, Kim JK, Hong Y, Park SHAntonie Van Leeuwenhoek10.1007/s10482-021-01646-z2021Animals, Bacterial Typing Techniques, Clostridiales, DNA, Bacterial/genetics, *Fatty Acids/analysis, Feces, *Phospholipids, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, SwineTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
16136Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22094)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22094
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
29535Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604125932
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
75814Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID370145.1StrainInfo: A central database for resolving microbial strain identifiers