Strain identifier
BacDive ID: 6321
Type strain:
Species: Kineosporia aurantiaca
Strain Designation: A/10312
Strain history: KCC A-0230 <-- H. Pagani A/10312.
NCBI tax ID(s): 1306174 (strain), 49185 (species)
General
@ref: 11340
BacDive-ID: 6321
DSM-Number: 43858
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, Gram-positive
description: Kineosporia aurantiaca A/10312 is a spore-forming, mesophilic, Gram-positive bacterium that was isolated from soil.
NCBI tax id
NCBI tax id | Matching level |
---|---|
1306174 | strain |
49185 | species |
strain history
@ref | history |
---|---|
11340 | <- JCM <- KCC <- H. Pagani, A/10312 |
67770 | KCC A-0230 <-- H. Pagani A/10312. |
doi: 10.13145/bacdive6321.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kineosporiales
- family: Kineosporiaceae
- genus: Kineosporia
- species: Kineosporia aurantiaca
- full scientific name: Kineosporia aurantiaca Pagani and Parenti 1978 (Approved Lists 1980)
@ref: 11340
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Kineosporiaceae
genus: Kineosporia
species: Kineosporia aurantiaca
full scientific name: Kineosporia aurantiaca Pagani and Parenti 1978 emend. Itoh et al. 1989
strain designation: A/10312
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: positive
- confidence: 96.663
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
19969 | Pastel orange (2003) | 10-14 days | ISP 2 |
19969 | Pastel orange (2003) | 10-14 days | ISP 3 |
19969 | Pastel orange (2003) | 10-14 days | ISP 4 |
19969 | Pastel orange (2003) | 10-14 days | ISP 5 |
19969 | Vermillion (2002) | 10-14 days | ISP 6 |
19969 | Pastel orange (2003) | 10-14 days | ISP 7 |
multicellular morphology
@ref | forms multicellular complex | medium name |
---|---|---|
19969 | no | ISP 2 |
19969 | no | ISP 3 |
19969 | no | ISP 4 |
19969 | no | ISP 5 |
19969 | no | ISP 6 |
19969 | no | ISP 7 |
multimedia
- @ref: 11340
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_43858.jpg
- caption: Medium 214 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
11340 | GYM+S MEDIUM (DSMZ Medium 214) | yes | https://mediadive.dsmz.de/medium/214 | Name: GYM+S MEDIUM (DSMZ Medium 214) Composition: Starch 20.0 g/l Agar 12.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
19969 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
19969 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
19969 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
19969 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
19969 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
19969 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
19969 | positive | optimum | 28 | mesophilic |
11340 | positive | growth | 28 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | yes | 100 |
69480 | no | 99.977 |
halophily
- @ref: 19969
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0 %
observation
- @ref: 67770
- observation: quinones: MK-9(H4)
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity |
---|---|---|---|
19969 | 62968 | cellulose | - |
19969 | 16634 | raffinose | + |
19969 | 26546 | rhamnose | + |
19969 | 28757 | fructose | + |
19969 | 29864 | mannitol | + |
19969 | 17268 | myo-inositol | + |
19969 | 18222 | xylose | - |
19969 | 17992 | sucrose | + |
19969 | 22599 | arabinose | + |
19969 | 17234 | glucose | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
11340 | soil | St. Raphael | France | FRA | Europe |
67770 | Soil | Tende | France | FRA | Europe |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
taxonmaps
- @ref: 69479
- File name: preview.99_24589.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_10254;97_12376;98_15213;99_24589&stattab=map
- Last taxonomy: Kineosporia aurantiaca
- 16S sequence: X87110
- Sequence Identity:
- Total samples: 541
- soil counts: 284
- aquatic counts: 68
- animal counts: 152
- plant counts: 37
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
11340 | 1 | Risk group (German classification) |
19969 | 1 | German classification |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | K.aurantiaca 16S rRNA gene | X87110 | 1501 | ena | 1306174 |
20218 | Kineosporia aurantiaca gene for 16S ribosomal RNA, partial sequence | D86937 | 1469 | ena | 1306174 |
20218 | Kineosporia aurantiaca gene for 16S rRNA, partial sequence, strain:JCM3230 | AB003931 | 1472 | ena | 1306174 |
20218 | Kineosporia aurantiaca strain NRRL B-16913 16S ribosomal RNA gene, partial sequence | AF095336 | 1513 | ena | 49185 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Kineosporia aurantiaca JCM 3230 | GCA_001315325 | contig | ncbi | 1306174 |
66792 | Kineosporia aurantiaca JCM 3230 | 1306174.4 | wgs | patric | 1306174 |
66792 | Kineosporia aurantiaca JCM 3230 | 1306174.8 | wgs | patric | 1306174 |
66792 | Kineosporia aurantiaca JCM 3230 NRRL B-16913 | 2772190730 | draft | img | 1306174 |
66792 | Kineosporia aurantiaca JCM 3230 | 2734481982 | draft | img | 1306174 |
67770 | Kineosporia aurantiaca JCM 3230 NRRL B-16913 | GCA_000719025 | contig | ncbi | 1306174 |
GC content
@ref | GC-content | method |
---|---|---|
67770 | 69.5 | high performance liquid chromatography (HPLC) |
67770 | 69.2 | high performance liquid chromatography (HPLC) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 100 | no |
motile | yes | 50.776 | no |
gram-positive | yes | 90.9 | no |
anaerobic | no | 99.424 | no |
halophile | no | 97.12 | yes |
spore-forming | yes | 87.596 | no |
thermophile | no | 99.724 | yes |
glucose-util | yes | 87.942 | yes |
flagellated | no | 93.101 | no |
aerobic | yes | 92.085 | no |
glucose-ferment | no | 86.398 | no |
External links
@ref: 11340
culture collection no.: DSM 43858, ATCC 29727, IFO 13890, IFO 14067, JCM 3230, KCC A-0230, NBRC 13890, NBRC 14067, NRRL B-16913, BCRC 13431, HUT 6563, IMSNU 21365, IMSNU 22002, KCTC 9189, KCTC 9364, NCIMB 11473, VKM Ac-702
straininfo link
- @ref: 75795
- straininfo: 39682
literature
- topic: Phylogeny
- Pubmed-ID: 15797143
- title: A new neutral polymer from the cell wall of actinomycete Kineosporia aurantiaca VKM Ac-702T.
- authors: Tul'skaya EM, Senchenkova SN, Evtushenko LI, Shashkov AS, Naumova IB
- journal: Carbohydr Res
- DOI: 10.1016/j.carres.2005.02.002
- year: 2005
- mesh: Acetylation, Actinomycetales/*chemistry/*classification, Biopolymers/*chemistry, Cell Wall/*chemistry, Galactose/analogs & derivatives, Magnetic Resonance Spectroscopy, Mannans/*chemistry
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
11340 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43858) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-43858 | |||
19969 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM43858.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
75795 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID39682.1 | StrainInfo: A central database for resolving microbial strain identifiers |