Strain identifier

BacDive ID: 6321

Type strain: Yes

Species: Kineosporia aurantiaca

Strain Designation: A/10312

Strain history: KCC A-0230 <-- H. Pagani A/10312.

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General

@ref: 11340

BacDive-ID: 6321

DSM-Number: 43858

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, Gram-positive

description: Kineosporia aurantiaca A/10312 is a spore-forming, mesophilic, Gram-positive bacterium that was isolated from soil.

NCBI tax id

NCBI tax idMatching level
1306174strain
49185species

strain history

@refhistory
11340<- JCM <- KCC <- H. Pagani, A/10312
67770KCC A-0230 <-- H. Pagani A/10312.

doi: 10.13145/bacdive6321.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kineosporiales
  • family: Kineosporiaceae
  • genus: Kineosporia
  • species: Kineosporia aurantiaca
  • full scientific name: Kineosporia aurantiaca Pagani and Parenti 1978 (Approved Lists 1980)

@ref: 11340

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Kineosporiaceae

genus: Kineosporia

species: Kineosporia aurantiaca

full scientific name: Kineosporia aurantiaca Pagani and Parenti 1978 emend. Itoh et al. 1989

strain designation: A/10312

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 96.663

colony morphology

@refcolony colorincubation periodmedium used
19969Pastel orange (2003)10-14 daysISP 2
19969Pastel orange (2003)10-14 daysISP 3
19969Pastel orange (2003)10-14 daysISP 4
19969Pastel orange (2003)10-14 daysISP 5
19969Vermillion (2002)10-14 daysISP 6
19969Pastel orange (2003)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
19969noISP 2
19969noISP 3
19969noISP 4
19969noISP 5
19969noISP 6
19969noISP 7

multimedia

  • @ref: 11340
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_43858.jpg
  • caption: Medium 214 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11340GYM+S MEDIUM (DSMZ Medium 214)yeshttps://mediadive.dsmz.de/medium/214Name: GYM+S MEDIUM (DSMZ Medium 214) Composition: Starch 20.0 g/l Agar 12.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19969ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19969ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19969ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19969ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19969ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19969ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
19969positiveoptimum28mesophilic
11340positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481yes100
69480no99.977

halophily

  • @ref: 19969
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activity
1996962968cellulose-
1996916634raffinose+
1996926546rhamnose+
1996928757fructose+
1996929864mannitol+
1996917268myo-inositol+
1996918222xylose-
1996917992sucrose+
1996922599arabinose+
1996917234glucose+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
11340soilSt. RaphaelFranceFRAEurope
67770SoilTendeFranceFRAEurope

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_24589.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_10254;97_12376;98_15213;99_24589&stattab=map
  • Last taxonomy: Kineosporia aurantiaca
  • 16S sequence: X87110
  • Sequence Identity:
  • Total samples: 541
  • soil counts: 284
  • aquatic counts: 68
  • animal counts: 152
  • plant counts: 37

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
113401Risk group (German classification)
199691German classification

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218K.aurantiaca 16S rRNA geneX871101501ena1306174
20218Kineosporia aurantiaca gene for 16S ribosomal RNA, partial sequenceD869371469ena1306174
20218Kineosporia aurantiaca gene for 16S rRNA, partial sequence, strain:JCM3230AB0039311472ena1306174
20218Kineosporia aurantiaca strain NRRL B-16913 16S ribosomal RNA gene, partial sequenceAF0953361513ena49185

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Kineosporia aurantiaca JCM 3230GCA_001315325contigncbi1306174
66792Kineosporia aurantiaca JCM 32301306174.4wgspatric1306174
66792Kineosporia aurantiaca JCM 32301306174.8wgspatric1306174
66792Kineosporia aurantiaca JCM 3230 NRRL B-169132772190730draftimg1306174
66792Kineosporia aurantiaca JCM 32302734481982draftimg1306174
67770Kineosporia aurantiaca JCM 3230 NRRL B-16913GCA_000719025contigncbi1306174

GC content

@refGC-contentmethod
6777069.5high performance liquid chromatography (HPLC)
6777069.2high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes50.776no
gram-positiveyes90.9no
anaerobicno99.424no
halophileno97.12yes
spore-formingyes87.596no
thermophileno99.724yes
glucose-utilyes87.942yes
flagellatedno93.101no
aerobicyes92.085no
glucose-fermentno86.398no

External links

@ref: 11340

culture collection no.: DSM 43858, ATCC 29727, IFO 13890, IFO 14067, JCM 3230, KCC A-0230, NBRC 13890, NBRC 14067, NRRL B-16913, BCRC 13431, HUT 6563, IMSNU 21365, IMSNU 22002, KCTC 9189, KCTC 9364, NCIMB 11473, VKM Ac-702

straininfo link

  • @ref: 75795
  • straininfo: 39682

literature

  • topic: Phylogeny
  • Pubmed-ID: 15797143
  • title: A new neutral polymer from the cell wall of actinomycete Kineosporia aurantiaca VKM Ac-702T.
  • authors: Tul'skaya EM, Senchenkova SN, Evtushenko LI, Shashkov AS, Naumova IB
  • journal: Carbohydr Res
  • DOI: 10.1016/j.carres.2005.02.002
  • year: 2005
  • mesh: Acetylation, Actinomycetales/*chemistry/*classification, Biopolymers/*chemistry, Cell Wall/*chemistry, Galactose/analogs & derivatives, Magnetic Resonance Spectroscopy, Mannans/*chemistry

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11340Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43858)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43858
19969Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM43858.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
75795Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID39682.1StrainInfo: A central database for resolving microbial strain identifiers