Strain identifier

BacDive ID: 6271

Type strain: Yes

Species: Knoellia aerolata

Strain Designation: 5317S-21

Strain history: KACC 20583 <-- S.-W. Kwon 5317S-21.

NCBI tax ID(s): 1385519 (strain), 442954 (species)

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General

@ref: 7592

BacDive-ID: 6271

DSM-Number: 18566

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, ovoid-shaped

description: Knoellia aerolata 5317S-21 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from air sample.

NCBI tax id

NCBI tax idMatching level
442954species
1385519strain

strain history

@refhistory
7592<- Soon-Wo Kwon; 5317S-21
67770KACC 20583 <-- S.-W. Kwon 5317S-21.

doi: 10.13145/bacdive6271.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Intrasporangiaceae
  • genus: Knoellia
  • species: Knoellia aerolata
  • full scientific name: Knoellia aerolata Weon et al. 2007

@ref: 7592

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Intrasporangiaceae

genus: Knoellia

species: Knoellia aerolata

full scientific name: Knoellia aerolata Weon et al. 2007 emend. Nouioui et al. 2018

strain designation: 5317S-21

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
32308positive1.5 µm1 µmovoid-shapedno
69480no93.244
69480positive100

Culture and growth conditions

culture medium

  • @ref: 7592
  • name: R2A MEDIUM (DSMZ Medium 830)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/830
  • composition: Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
7592positivegrowth28mesophilic
18321positiveoptimum28mesophilic
32308positivegrowth05-35
32308positiveoptimum30mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
32308positivegrowth5.0-9.0alkaliphile
32308positiveoptimum6.5

Physiology and metabolism

oxygen tolerance

  • @ref: 32308
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
32308no
69481no100
69480no99.989

halophily

@refsaltgrowthtested relationconcentration
32308NaClpositivegrowth0-2 %
32308NaClpositiveoptimum0.5 %

murein

  • @ref: 7592
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

observation

  • @ref: 67770
  • observation: quinones: MK-8(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
32308370543-hydroxybutyrate+carbon source
3230830089acetate+carbon source
3230816449alanine+carbon source
3230824265gluconate+carbon source
3230817234glucose+carbon source
3230828087glycogen+carbon source
3230817306maltose+carbon source
3230829864mannitol+carbon source
3230837684mannose+carbon source
32308506227N-acetylglucosamine+carbon source
3230826271proline+carbon source
3230817272propionate+carbon source
3230817814salicin+carbon source
3230830911sorbitol+carbon source
3230817992sucrose+carbon source
3230853426tween 80+carbon source
3230831011valerate+carbon source
323084853esculin+hydrolysis
3230817632nitrate+reduction
6837917632nitrate-reduction
683794853esculin+hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose+fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
32308alkaline phosphatase+3.1.3.1
32308gelatinase+
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase+3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18321---++-+-+-+------+-

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18321+++-+++--+--++++---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
7592air sampleSuwonRepublic of KoreaKORAsia
67770Air sampleSuwon CityRepublic of KoreaKORAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Air

taxonmaps

  • @ref: 69479
  • File name: preview.99_6129.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_1391;97_1657;98_4546;99_6129&stattab=map
  • Last taxonomy: Knoellia
  • 16S sequence: EF553529
  • Sequence Identity:
  • Total samples: 1491
  • soil counts: 348
  • aquatic counts: 244
  • animal counts: 892
  • plant counts: 7

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
183211German classification
75921Risk group (German classification)

Sequence information

16S sequences

  • @ref: 7592
  • description: Knoellia aerolata strain 5317S-21 16S ribosomal RNA gene, partial sequence
  • accession: EF553529
  • length: 1401
  • database: ena
  • NCBI tax ID: 442954

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Knoellia aerolata DSM 185661385519.3wgspatric1385519
66792Knoellia aerolata DSM 185662639762785draftimg1385519
67770Knoellia aerolata DSM 18566GCA_000768695contigncbi1385519

GC content

@refGC-contentmethod
759273
6777071.3genome sequence analysis
6777073high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno93.88yes
gram-positiveyes88.307yes
anaerobicno98.946yes
halophileno95.427yes
spore-formingno93.784no
glucose-utilyes86.438yes
thermophileno96.911yes
aerobicyes92.628yes
flagellatedno98.206yes
glucose-fermentno89.008yes

External links

@ref: 7592

culture collection no.: DSM 18566, KACC 20583, JCM 16377, NBRC 106306

straininfo link

  • @ref: 75748
  • straininfo: 398903

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18048739Knoellia aerolata sp. nov., isolated from an air sample in Korea.Weon HY, Kim BY, Schumann P, Kroppenstedt RM, Noh HJ, Park CW, Kwon SWInt J Syst Evol Microbiol10.1099/ijs.0.65293-02007Actinomycetales/chemistry/*classification/genetics/*isolation & purification, *Air Microbiology, Bacterial Typing Techniques, Base Composition, Carbohydrates/analysis, Cell Wall/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Genes, rRNA, Hydrogen-Ion Concentration, Korea, Locomotion/physiology, Molecular Sequence Data, Mycolic Acids/analysis, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sodium Chloride/metabolism, Spores, Bacterial/cytology, TemperatureGenetics
Phylogeny21421930Knoellia locipacati sp. nov., from soil of the Demilitarized Zone in South Korea.Shin NR, Roh SW, Kim MS, Jung MJ, Whon TW, Bae JWInt J Syst Evol Microbiol10.1099/ijs.0.031880-02011Actinomycetales/chemistry/*classification/genetics/*isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Genes, rRNA, Genotype, Lipids/analysis, *Military Facilities, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Species SpecificityGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
7592Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18566)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18566
18321Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM18566.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32308Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2854628776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
75748Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID398903.1StrainInfo: A central database for resolving microbial strain identifiers