Strain identifier
BacDive ID: 6249
Type strain:
Species: Janibacter limosus
Strain Designation: 2003-70
Strain history: CIP <- 1997, K. Martin, Hans Knöll Inst., Jena, Germany: strain HKI 84
NCBI tax ID(s): 53458 (species)
General
@ref: 4221
BacDive-ID: 6249
DSM-Number: 11141
keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive, coccus-shaped
description: Janibacter limosus 2003-70 is an obligate aerobe, mesophilic, Gram-positive bacterium that was isolated from sludge of waste water treatment.
NCBI tax id
- NCBI tax id: 53458
- Matching level: species
strain history
@ref | history |
---|---|
4221 | <- K. Martin, 2003-70 (= HKI 84) |
39244 | 1997, K. Martin, H. Knöll Inst., Jena, Germany: strain HKI 84 |
120608 | CIP <- 1997, K. Martin, Hans Knöll Inst., Jena, Germany: strain HKI 84 |
doi: 10.13145/bacdive6249.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Micrococcales
- family: Intrasporangiaceae
- genus: Janibacter
- species: Janibacter limosus
- full scientific name: Janibacter limosus Martin et al. 1997
@ref: 4221
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Intrasporangiaceae
genus: Janibacter
species: Janibacter limosus
full scientific name: Janibacter limosus Martin et al. 1997
strain designation: 2003-70
type strain: no
Morphology
cell morphology
- @ref: 120608
- gram stain: positive
- cell shape: coccus-shaped
- motility: no
colony morphology
- @ref: 120608
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
4221 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
39244 | MEDIUM 112 - for Janibacter | yes | Distilled water make up to (1000.000 ml);Magnesium sulphate heptahydrate (1.000 g);Agar (20.000 g);Yeast extract (5.000 g);Peptone (10.000 g);Tween 80 (50.000 mg);Glycerol (2.000 g);Malt extract (5.000 g);Beef extract (2.000 g);Casamino acids (5.000 g);Ca | |
120608 | CIP Medium 112 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=112 | |
120608 | CIP Medium 235 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=235 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
4221 | positive | growth | 28 | mesophilic |
39244 | positive | growth | 30 | mesophilic |
120608 | positive | growth | 25-37 | mesophilic |
120608 | no | growth | 10 | psychrophilic |
120608 | no | growth | 15 | psychrophilic |
120608 | no | growth | 41 | thermophilic |
120608 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 120608
- oxygen tolerance: obligate aerobe
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
120608 | NaCl | positive | growth | 0-6 % |
120608 | NaCl | no | growth | 8 % |
120608 | NaCl | no | growth | 10 % |
murein
- @ref: 4221
- murein short key: A31
- type: A1gamma m-Dpm-direct
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
120608 | 4853 | esculin | - | hydrolysis |
120608 | 606565 | hippurate | - | hydrolysis |
120608 | 17632 | nitrate | + | reduction |
120608 | 16301 | nitrite | - | reduction |
120608 | 17632 | nitrate | - | respiration |
metabolite production
- @ref: 120608
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | methylred-test |
---|---|---|---|---|
120608 | 15688 | acetoin | - | |
120608 | 17234 | glucose | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
120608 | oxidase | - | |
120608 | beta-galactosidase | - | 3.2.1.23 |
120608 | alcohol dehydrogenase | - | 1.1.1.1 |
120608 | gelatinase | - | |
120608 | amylase | - | |
120608 | DNase | - | |
120608 | caseinase | - | 3.4.21.50 |
120608 | catalase | + | 1.11.1.6 |
120608 | tween esterase | - | |
120608 | gamma-glutamyltransferase | + | 2.3.2.2 |
120608 | lecithinase | - | |
120608 | lipase | - | |
120608 | lysine decarboxylase | - | 4.1.1.18 |
120608 | ornithine decarboxylase | - | 4.1.1.17 |
120608 | phenylalanine ammonia-lyase | - | 4.3.1.24 |
120608 | tryptophan deaminase | - | |
120608 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
120608 | - | + | + | + | - | + | - | - | - | - | + | + | - | - | - | - | - | - | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
120608 | + | - | - | + | + | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | + | - | + | - | - | - | - | + | - | - | - | - | - | - | - | + | + | - | + | + | + | + | - | + | + | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent | geographic location | isolation date |
---|---|---|---|---|---|---|
4221 | sludge of waste water treatment | Germany | DEU | Europe | ||
120608 | Environment, Sludge from wastwater treatment plant | Germany | DEU | Europe | Jena | 1992 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Waste | #Wastewater |
#Environmental | #Terrestrial | #Mud (Sludge) |
taxonmaps
- @ref: 69479
- File name: preview.99_2651.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_927;97_1079;98_2052;99_2651&stattab=map
- Last taxonomy: Janibacter limosus subclade
- 16S sequence: Y08540
- Sequence Identity:
- Total samples: 18424
- soil counts: 7408
- aquatic counts: 3591
- animal counts: 5312
- plant counts: 2113
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
4221 | 1 | Risk group (German classification) |
120608 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 4221
- description: J.thuringensis 16S rRNA gene
- accession: Y08540
- length: 1473
- database: ena
- NCBI tax ID: 53458
External links
@ref: 4221
culture collection no.: DSM 11141, HKI 84, CIP 105277, ATCC 700322
straininfo link
- @ref: 75727
- straininfo: 45418
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
4221 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11141) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-11141 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
39244 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/17279 | ||||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
75727 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID45418.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
120608 | Curators of the CIP | Collection of Institut Pasteur (CIP 105277) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105277 |