Strain identifier

BacDive ID: 6249

Type strain: No

Species: Janibacter limosus

Strain Designation: 2003-70

Strain history: CIP <- 1997, K. Martin, Hans Knöll Inst., Jena, Germany: strain HKI 84

NCBI tax ID(s): 53458 (species)

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General

@ref: 4221

BacDive-ID: 6249

DSM-Number: 11141

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive, coccus-shaped

description: Janibacter limosus 2003-70 is an obligate aerobe, mesophilic, Gram-positive bacterium that was isolated from sludge of waste water treatment.

NCBI tax id

  • NCBI tax id: 53458
  • Matching level: species

strain history

@refhistory
4221<- K. Martin, 2003-70 (= HKI 84)
392441997, K. Martin, H. Knöll Inst., Jena, Germany: strain HKI 84
120608CIP <- 1997, K. Martin, Hans Knöll Inst., Jena, Germany: strain HKI 84

doi: 10.13145/bacdive6249.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Intrasporangiaceae
  • genus: Janibacter
  • species: Janibacter limosus
  • full scientific name: Janibacter limosus Martin et al. 1997

@ref: 4221

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Intrasporangiaceae

genus: Janibacter

species: Janibacter limosus

full scientific name: Janibacter limosus Martin et al. 1997

strain designation: 2003-70

type strain: no

Morphology

cell morphology

  • @ref: 120608
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 120608

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4221TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
39244MEDIUM 112 - for JanibacteryesDistilled water make up to (1000.000 ml);Magnesium sulphate heptahydrate (1.000 g);Agar (20.000 g);Yeast extract (5.000 g);Peptone (10.000 g);Tween 80 (50.000 mg);Glycerol (2.000 g);Malt extract (5.000 g);Beef extract (2.000 g);Casamino acids (5.000 g);Ca
120608CIP Medium 112yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=112
120608CIP Medium 235yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=235

culture temp

@refgrowthtypetemperaturerange
4221positivegrowth28mesophilic
39244positivegrowth30mesophilic
120608positivegrowth25-37mesophilic
120608nogrowth10psychrophilic
120608nogrowth15psychrophilic
120608nogrowth41thermophilic
120608nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120608
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
120608NaClpositivegrowth0-6 %
120608NaClnogrowth8 %
120608NaClnogrowth10 %

murein

  • @ref: 4221
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1206084853esculin-hydrolysis
120608606565hippurate-hydrolysis
12060817632nitrate+reduction
12060816301nitrite-reduction
12060817632nitrate-respiration

metabolite production

  • @ref: 120608
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12060815688acetoin-
12060817234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
120608oxidase-
120608beta-galactosidase-3.2.1.23
120608alcohol dehydrogenase-1.1.1.1
120608gelatinase-
120608amylase-
120608DNase-
120608caseinase-3.4.21.50
120608catalase+1.11.1.6
120608tween esterase-
120608gamma-glutamyltransferase+2.3.2.2
120608lecithinase-
120608lipase-
120608lysine decarboxylase-4.1.1.18
120608ornithine decarboxylase-4.1.1.17
120608phenylalanine ammonia-lyase-4.3.1.24
120608tryptophan deaminase-
120608urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120608-+++-+----++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120608+--++--+--------------+------------+-------------+-----------------+-----+-+----+-------++-++++-++-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic locationisolation date
4221sludge of waste water treatmentGermanyDEUEurope
120608Environment, Sludge from wastwater treatment plantGermanyDEUEuropeJena1992

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Wastewater
#Environmental#Terrestrial#Mud (Sludge)

taxonmaps

  • @ref: 69479
  • File name: preview.99_2651.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_927;97_1079;98_2052;99_2651&stattab=map
  • Last taxonomy: Janibacter limosus subclade
  • 16S sequence: Y08540
  • Sequence Identity:
  • Total samples: 18424
  • soil counts: 7408
  • aquatic counts: 3591
  • animal counts: 5312
  • plant counts: 2113

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
42211Risk group (German classification)
1206081Risk group (French classification)

Sequence information

16S sequences

  • @ref: 4221
  • description: J.thuringensis 16S rRNA gene
  • accession: Y08540
  • length: 1473
  • database: ena
  • NCBI tax ID: 53458

External links

@ref: 4221

culture collection no.: DSM 11141, HKI 84, CIP 105277, ATCC 700322

straininfo link

  • @ref: 75727
  • straininfo: 45418

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4221Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11141)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11141
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39244Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17279
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75727Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID45418.1StrainInfo: A central database for resolving microbial strain identifiers
120608Curators of the CIPCollection of Institut Pasteur (CIP 105277)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105277