Strain identifier
BacDive ID: 6148
Type strain: 
Species: Acidihalobacter prosperus
Strain Designation: LM3
Strain history: DSM 5130 <-- K. O. Stetter LM3.
NCBI tax ID(s): 160660 (species)
version 8.1 (current version)
General
@ref: 1930
BacDive-ID: 6148
DSM-Number: 5130
keywords: genome sequence, 16S sequence, Bacteria, mesophilic
description: Acidihalobacter prosperus LM3 is a mesophilic bacterium that was isolated from geothermally heated sea sediment.
NCBI tax id
- NCBI tax id: 160660
- Matching level: species
strain history
| @ref | history |
|---|---|
| 1930 | <- K. O. Stetter and H. Huber, Univ. Regensburg, Germany; LM3 {1983} |
| 67770 | DSM 5130 <-- K. O. Stetter LM3. |
doi: 10.13145/bacdive6148.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Chromatiales
- family: Ectothiorhodospiraceae
- genus: Acidihalobacter
- species: Acidihalobacter prosperus
- full scientific name: Acidihalobacter prosperus Cárdenas et al. 2015
synonyms
- @ref: 20215
- synonym: Thiobacillus prosperus
@ref: 1930
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Hydrogenophilales
family: Ectothiorhodospiraceae
genus: Acidihalobacter
species: Acidihalobacter prosperus
full scientific name: Acidihalobacter prosperus Cárdenas et al. 2015
strain designation: LM3
type strain: yes
Morphology
colony morphology
- @ref: 1930
- incubation period: 3-7 days
Culture and growth conditions
culture medium
- @ref: 1930
- name: ACIDIHALOBACTER PROSPERUS MEDIUM (DSMZ Medium 477)
- growth: yes
- link: https://mediadive.dsmz.de/medium/477
- composition: Name: ACIDIHALOBACTER PROSPERUS MEDIUM (DSMZ Medium 477) Composition: FeSO4 x 7 H2O 19.9601 g/l MgCl2 x 6 H2O 2.74451 g/l NH4Cl 1.2475 g/l KCl 0.329341 g/l K2HPO4 0.139721 g/l KH2PO4 0.139721 g/l MgSO4 x 7 H2O 0.0299401 g/l Nitrilotriacetic acid 0.0149701 g/l NaCl 0.00998004 g/l MnSO4 x H2O 0.00499002 g/l NiCl2 x 6 H2O 0.00199601 g/l CoSO4 x 7 H2O 0.00179641 g/l ZnSO4 x 7 H2O 0.00179641 g/l CaCl2 x 2 H2O 0.000998004 g/l AlK(SO4)2 x 12 H2O 0.000199601 g/l CuSO4 x 5 H2O 9.98004e-05 g/l H3BO3 9.98004e-05 g/l Na2MoO4 x 2 H2O 9.98004e-05 g/l Na2WO4 x 2 H2O 3.99202e-06 g/l Na2SeO3 x 5 H2O 2.99401e-06 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 1930 | positive | growth | 35 | mesophilic |
| 67770 | positive | growth | 35 | mesophilic |
Physiology and metabolism
observation
- @ref: 67770
- observation: quinones: Q-8
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 1930 | geothermally heated sea sediment | Vulcano, beach of Porto di Levante | Italy | ITA | Europe |
| 67770 | Shallow geothermally heated seafloor at the beach of Porto di Levante | Vulcano | Italy | ITA | Europe |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Marine |
| #Environmental | #Aquatic | #Sediment |
taxonmaps
- @ref: 69479
- File name: preview.99_6292.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_56;96_1688;97_2021;98_3960;99_6292&stattab=map
- Last taxonomy: Acidihalobacter prosperus
- 16S sequence: AY034139
- Sequence Identity:
- Total samples: 51
- soil counts: 12
- aquatic counts: 35
- animal counts: 2
- plant counts: 2
Safety information
risk assessment
- @ref: 1930
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Thiobacillus prosperus 16S ribosomal RNA gene, partial sequence | AY034139 | 1433 | ena | 160660 |
| 1930 | Thiobacillus prosperus strain DSM 5130 16S ribosomal RNA gene, partial sequence | EU653291 | 1461 | ena | 160660 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Thiobacillus prosperus strain DSM 5130 | 160660.4 | wgs | patric | 160660 |
| 66792 | Acidihalobacter prosperus DSM 5130 | 2648501264 | draft | img | 160660 |
| 67770 | Acidihalobacter prosperus DSM 5130 | GCA_000754095 | contig | ncbi | 160660 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 1930 | 64.0 | |
| 67770 | 64.4 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | yes | 92.4 | no |
| gram-positive | no | 99.024 | no |
| anaerobic | no | 97.062 | no |
| aerobic | no | 55.326 | no |
| halophile | no | 80.926 | no |
| spore-forming | no | 95.241 | no |
| thermophile | no | 95.028 | yes |
| glucose-util | yes | 62.969 | no |
| flagellated | yes | 60.181 | no |
| glucose-ferment | no | 87.398 | no |
External links
@ref: 1930
culture collection no.: DSM 5130, JCM 30709
straininfo link
- @ref: 75635
- straininfo: 47898
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Genetics | 25342676 | Draft Genome Sequence of the Iron-Oxidizing, Acidophilic, and Halotolerant "Thiobacillus prosperus" Type Strain DSM 5130. | Ossandon FJ, Cardenas JP, Corbett M, Quatrini R, Holmes DS, Watkin E | Genome Announc | 10.1128/genomeA.01042-14 | 2014 | ||
| Phylogeny | 26198437 | Reclassification of 'Thiobacillus prosperus' Huber and Stetter 1989 as Acidihalobacter prosperus gen. nov., sp. nov., a member of the family Ectothiorhodospiraceae. | Pablo Cardenas J, Ortiz R, Norris PR, Watkin E, Holmes DS | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000468 | 2015 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Ectothiorhodospiraceae/*classification, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Thiobacillus/*classification | Genetics |
| Metabolism | 30031896 | Quantitative proteomics using SWATH-MS identifies mechanisms of chloride tolerance in the halophilic acidophile Acidihalobacter prosperus DSM 14174. | Khaleque HN, Shafique R, Kaksonen AH, Boxall NJ, Watkin ELJ | Res Microbiol | 10.1016/j.resmic.2018.07.002 | 2018 | Acids/*metabolism, Bacterial Proteins/chemistry/*genetics/metabolism, Chlorides/*metabolism, DNA Repair, Gammaproteobacteria/chemistry/genetics/*metabolism, Mass Spectrometry, Proteomics, Sodium Chloride/*metabolism | |
| 35516430 | Prediction and Inferred Evolution of Acid Tolerance Genes in the Biotechnologically Important Acidihalobacter Genus. | Boase K, Gonzalez C, Vergara E, Neira G, Holmes D, Watkin E | Front Microbiol | 10.3389/fmicb.2022.848410 | 2022 |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 1930 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5130) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-5130 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 75635 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID47898.1 | StrainInfo: A central database for resolving microbial strain identifiers |