Strain identifier

BacDive ID: 6139

Type strain: Yes

Species: Tepidiphilus margaritifer

Strain Designation: N2-214

Culture col. no.: DSM 15129, LMG 21637

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 5.0 (current version):
version 4.1:
version 4.0:
version 3.0:
version 2.1:
version 2.0:
version 1.0:
version 5.0 (current version)

General

@ref: 5722

BacDive-ID: 6139

DSM-Number: 15129

keywords: Bacteria, 16S sequence, genome sequence, thermophilic

description: Tepidiphilus margaritifer N2-214 is a thermophilic bacterium that was isolated from thermophilic digester of wastewater-treatment sludge.

strain history: <- C. M. Manaia; N2-214

doi: 10.13145/bacdive6139.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/proteobacteria
  • domain: Bacteria
  • phylum: Proteobacteria
  • class: Hydrogenophilalia
  • order: Hydrogenophilales
  • family: Hydrogenophilaceae
  • genus: Tepidiphilus
  • species: Tepidiphilus margaritifer
  • full scientific name: Tepidiphilus margaritifer Manaia et al. 2003

@ref: 5722

domain: Bacteria

phylum: Proteobacteria

class: Hydrogenophilalia

order: Hydrogenophilales

family: Hydrogenophilaceae

genus: Tepidiphilus

species: Tepidiphilus margaritifer

full scientific name: Tepidiphilus margaritifer Manaia et al. 2003 emend. Poddar et al. 2014

strain designation: N2-214

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 5722
  • name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)
  • growth: yes
  • link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium381.pdf

culture temp

  • @ref: 5722
  • growth: positive
  • type: growth
  • temperature: 50
  • range: thermophilic

Isolation, sampling and environmental information

isolation

  • @ref: 5722
  • sample type: thermophilic digester of wastewater-treatment sludge
  • geographic location: Porto
  • country: Portugal
  • origin.country: PRT
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Engineered#Biodegradation
#Engineered#Waste#Wastewater
#Environmental#Aquatic#Mud (Sludge)
#Condition#Thermophilic (>45°C)

Safety information

risk assessment

  • @ref: 5722
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 5722
  • description: Tepidiphilus margaritifer partial 16S rRNA gene, type strain N2-214T
  • accession: AJ504663
  • length: 1456
  • database: ena
  • NCBI tax ID: 1123354

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Tepidiphilus margaritifer DSM 15129GCA_000425565scaffoldncbi1123354
66792Tepidiphilus margaritifer DSM 151291123354.4wgspatric1123354
66792Tepidiphilus margaritifer DSM 151292524023151permanentimg1123354

GC content

  • @ref: 5722
  • GC-content: 64.8

External links

@ref: 5722

culture collection no.: DSM 15129, LMG 21637

straininfo link

@refpassport
20218http://www.straininfo.net/strains/371478
20218http://www.straininfo.net/strains/277875

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5722Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15129)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15129
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)