Strain identifier

BacDive ID: 6102

Type strain: Yes

Species: Helicobacter pylori

Strain Designation: RPH 13487

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 15441

BacDive-ID: 6102

DSM-Number: 21031

keywords: gram-negative, mesophilic, microaerophile, Bacteria, 16S sequence, genome sequence, rod-shaped

description: Helicobacter pylori RPH 13487 is a microaerophile, mesophilic, gram-negative bacterium that was isolated from endoscopic biopsy specimen of human gastric mucosa.

strain history: <- NCTC; NCTC 11637 <- C. S. Goodwin and D. I. Annear;

doi: 10.13145/bacdive6102.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/proteobacteria
  • domain: Bacteria
  • phylum: Proteobacteria
  • class: Epsilonproteobacteria
  • order: Campylobacterales
  • family: Helicobacteraceae
  • genus: Helicobacter
  • species: Helicobacter pylori
  • full scientific name: Helicobacter pylori (Marshall et al. 1985) Goodwin et al. 1989
  • synonyms

    @refsynonym
    20215Helicobacter nemestrinae
    20215Campylobacter pyloridis
    20215Campylobacter pylori

@ref: 15441

domain: Bacteria

phylum: Proteobacteria

class: Epsilonproteobacteria

order: Campylobacterales

family: Helicobacteraceae

genus: Helicobacter

species: Helicobacter pylori

full scientific name: Helicobacter pylori (Marshall et al. 1985) Goodwin et al. 1989

strain designation: RPH 13487

type strain: yes

Morphology

cell morphology

  • @ref: 22920
  • gram stain: negative
  • cell length: 2.5-5 µm
  • cell width: 0.5-1 µm
  • cell shape: rod-shaped
  • flagellum arrangement: polar

colony morphology

  • @ref: 15441
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
22920Brain heart infusionyes
15441COLUMBIA BLOOD MEDIUM (DSMZ Medium 693), liquid layer on top of agar necessaryyeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium693.pdf
41863MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)

culture temp

@refgrowthtypetemperaturerange
22920positivegrowth37mesophilic
15441positivegrowth37mesophilic
41863positivegrowth37mesophilic
46705positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 46705
  • oxygen tolerance: microaerophile

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
22920606565hippurate-hydrolysis
2292016199urea+hydrolysis
2292017632nitrate-reduction

metabolite production

  • @ref: 22920
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: no

enzymes

@refvalueactivityec
22920alkaline phosphatase-3.1.3.1
22920catalase+1.11.1.6
22920leucine arylamidase+/-3.4.11.1
22920cytochrome oxidase+1.9.3.1
22920urease+3.5.1.5
15441catalase+1.11.1.6
15441cytochrome-c oxidase+1.9.3.1

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    46705C12:00.612
    46705C13:00.513
    46705C14:050.914
    46705C15:01.115
    46705C16:0216
    46705C18:04.618
    46705C19:00.519
    46705C13:0 ISO 2OH0.513.814
    46705C13:1 at 12-130.912.931
    46705C16:0 3OH5.517.52
    46705C17:0 3OH0.818.535
    46705C18:0 3OH3.919.551
    46705C18:1 ω7c /12t/9t6.417.824
    46705C18:2 ω6,9c/C18:0 ANTE1.117.724
    46705C19:0 CYCLO ω8c19.718.9
    46705unknown 14.9661.114.966
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API CAM

@refURENITESTHIPGGTTTCPYRAArgAAspAPALH2SGLUSUTNALCFZACEPROPMLTCITEROCAT
15441+---+----+----------+
15441+---+----+----------+
15441+---+----+----------+
15441+---+----+--+/--------+
15441+-+-+----+----------+
15441+---+----+----------+
15441+---++---+----------+
15441+---+----+----------+
15441+---++---+----------+
15441+---+----+----------+
15441+---++---+----------+
15441+---++---+----------+
15441+---+----+----------+
15441+-+-+----+----------+
15441+--++----+----------+

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dategeographic location
15441endoscopic biopsy specimen of human gastric mucosaAustraliaAUSAustralia and Oceania
46705Human gastric biopsyAustraliaAUSAustralia and Oceania1982-05-05Perth

isolation source categories

Cat1Cat2Cat3
#Infection#Patient#Biopsy
#Infection#Patient#Specimen
#Host#Human
#Host Body-Site#Gastrointestinal tract#Stomach

Safety information

risk assessment

  • @ref: 15441
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Helicobacter pylori 16S ribosomal RNA gene, partial sequenceAF3021061445ena210
20218Helicobacter pylori (ATCC 43504) partial 16S ribosomal RNAM881571450ena210
20218Helicobacter pylori isolate ATCC 43504 16S rRNA geneU013301503ena210
20218H.pylori gene for 16S ribosomal RNA (partial)Z257411454ena102618
15441Helicobacter pylori strain ATCC 43504 16S ribosomal RNA, partial sequenceNR_0447611503nuccore210

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504GCA_900478295completencbi102618
66792Helicobacter pylori ATCC 43504GCA_004295525completencbi210
66792Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504GCA_000258845contigncbi102618
66792Helicobacter pylori NCTC 11637 = CCUG 17874102618.3wgspatric102618
66792Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504 strain NCTC11637102618.7completepatric102618
66792Helicobacter pylori strain ATCC 43504 strain PMSS1210.5630completepatric210
66792Helicobacter pylori strain PMSS1 strain ATCC 43504210.5631completepatric210

GC content

  • @ref: 22920
  • GC-content: 37.1

External links

@ref: 15441

culture collection no.: CCUG 15815, LMG 7540, CIP 103995, DSM 21031, ATCC 43504, CCUG 17874, DSM 4867, LMG 7539, NCTC 11637

straininfo link

@refpassport
20218http://www.straininfo.net/strains/49233
20218http://www.straininfo.net/strains/41195
20218http://www.straininfo.net/strains/151665
20218http://www.straininfo.net/strains/41198
20218http://www.straininfo.net/strains/49235

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15441Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21031)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21031
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
22920C. STEWART GOODWIN, JOHN A. ARMSTRONG, TERRY CHILVERS, MICHELLE PETERS, M. DAVID COLLINS, LINDSAY SLY, WILLIAM McCONNELL, WILLIAM E. S. HARPER10.1099/00207713-39-4-397Transfer of Campylobacter pylori and Campylobacter mustelae to Helicobacter gen. nov. as Helicobacter pylori comb. nov. and Helicobacter mustelae comb. nov., RespectivelyIJSEM 39: 397-405 1989
41863Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15853
46705Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 17874)https://www.ccug.se/strain?id=17874
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)