Strain identifier

BacDive ID: 6101

Type strain: No

Species: Campylobacter pylori

Strain Designation: RPH 13491

Strain history: CIP <- 2001, CCUG <- C.S. Goodwin, Perth, Western Australia

NCBI tax ID(s): 102619 (strain), 210 (species)

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General

@ref: 3863

BacDive-ID: 6101

DSM-Number: 10242

keywords: 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, motile, rod-shaped

description: Campylobacter pylori RPH 13491 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from human gastric antrum.

NCBI tax id

NCBI tax idMatching level
102619strain
210species

strain history

@refhistory
3863<- NCTC <- C. S. Goodwin
122082CIP <- 2001, CCUG <- C.S. Goodwin, Perth, Western Australia

doi: 10.13145/bacdive6101.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Epsilonproteobacteria
  • order: Campylobacterales
  • family: Campylobacteraceae
  • genus: Campylobacter
  • species: Campylobacter pylori
  • full scientific name: Campylobacter pylori corrig. Marshall et al. 1985

  • synonyms

    @refsynonym
    20215Campylobacter pyloridis
    20215Helicobacter pylori
    20215Helicobacter nemestrinae

@ref: 3863

domain: Bacteria

phylum: Proteobacteria

class: Epsilonproteobacteria

order: Campylobacterales

family: Helicobacteraceae

genus: Helicobacter

species: Helicobacter pylori

full scientific name: Helicobacter pylori (Marshall et al. 1985) Goodwin et al. 1989

strain designation: RPH 13491

type strain: no

Morphology

cell morphology

  • @ref: 122082
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

@refincubation period
38631-2 days
122082

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3863COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
36116MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
122082CIP Medium 45yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=45
122082CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
3863positivegrowth37mesophilic
36116positivegrowth37mesophilic
46706positivegrowth37mesophilic
122082positivegrowth37-41
122082nogrowth5psychrophilic
122082nogrowth10psychrophilic
122082nogrowth25mesophilic
122082nogrowth30mesophilic
122082nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
3863microaerophile
46706microaerophile
122082microaerophile

halophily

  • @ref: 122082
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 3.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
122082606565hippurate+hydrolysis
12208217632nitrate-reduction
12208216301nitrite-reduction
6837316199urea+hydrolysis
6837317632nitrate-reduction
6837317634D-glucose-assimilation
6837330031succinate-assimilation
6837330089acetate-assimilation
6837317272propionate-assimilation
6837325115malate-assimilation

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistant
6837348923erythromycinyesyes14 µg
68373474053cefazolinyesyes224 µg
68373100147nalidixic acidyesyes84 µg
1220820129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)yesnoyes

metabolite production

@refChebi-IDmetaboliteproduction
6837316136hydrogen sulfideno
12208235581indoleno

enzymes

@refvalueactivityec
3863catalase+1.11.1.6
3863cytochrome-c oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68373catalase+1.11.1.6
68373alkaline phosphatase+3.1.3.1
68373L-aspartate arylamidase-3.4.11.21
68373pyrrolidonyl arylamidase-3.4.19.3
68373gamma-glutamyltransferase+2.3.2.2
68373urease+3.5.1.5
122082oxidase+
122082catalase+1.11.1.6
122082gamma-glutamyltransferase+2.3.2.2
122082urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122082-+++-+-+--++--------

API CAM

@refURENITESTHIPGGTTTCPYRAArgAAspAPALH2SGLUSUTNALCFZACEPROPMLTCITEROCAT
3863+--+++---+----------+
3863+---+----+----------+
3863+---++---+----------+
3863+---++---+----------+
3863+---++---+--------+/--
3863+---++-+-+----------+
3863+---+----+----------+
46706+-+-++---+----------+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudesampling date
3863human gastric antrumPerthAustraliaAUSAustralia and Oceania-31.9535115.857
46706Human gastric biopsyPerth,FreemantleAustraliaAUSAustralia and Oceania1982-04-12
122082Human, GastricAustraliaAUSAustralia and Oceania

isolation source categories

Cat1Cat2
#Host#Human
#Host Body-Site#Gastrointestinal tract

taxonmaps

  • @ref: 69479
  • File name: preview.99_91.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_36;96_72;97_75;98_82;99_91&stattab=map
  • Last taxonomy: Helicobacter pylori subclade
  • 16S sequence: Z25742
  • Sequence Identity:
  • Total samples: 18084
  • soil counts: 670
  • aquatic counts: 829
  • animal counts: 16307
  • plant counts: 278

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
38632Risk group (German classification)
1220822Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: H.pylori gene for 16S ribosomal RNA (partial)
  • accession: Z25742
  • length: 1454
  • database: ena
  • NCBI tax ID: 210

External links

@ref: 3863

culture collection no.: DSM 10242, NCTC 11638, CCUG 17875, LMG 7542, CIP 107264, LMG 7541

straininfo link

  • @ref: 75589
  • straininfo: 48787

literature

Pubmed-IDtitleauthorsjournalDOIyearmeshtopictopic2
2753837The effect of cooling rate, freeze-drying suspending fluid and culture age on the preservation of Campylobacter pylori.Owen RJ, On SL, Costas MJ Appl Bacteriol10.1111/j.1365-2672.1989.tb02486.x1989*Campylobacter, Freeze Drying, *Preservation, Biological, Time Factors
3183019Campylobacter pylori isolated from the stomach of the monkey, Macaca nemestrina.Bronsdon MA, Schoenknecht FDJ Clin Microbiol10.1128/jcm.26.9.1725-1728.19881988Animals, Campylobacter/growth & development/isolation & purification, Campylobacter Infections/microbiology/*veterinary, Carrier State/microbiology/*veterinary, Disease Models, Animal, Gastritis/microbiology/*veterinary, *Macaca, *Macaca nemestrina, Monkey Diseases/*microbiologyEnzymologyPhylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3863Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10242)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10242
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36116Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4680
46706Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 17875)https://www.ccug.se/strain?id=17875
68373Automatically annotated from API CAM
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75589Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID48787.1StrainInfo: A central database for resolving microbial strain identifiers
122082Curators of the CIPCollection of Institut Pasteur (CIP 107264)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107264