Strain identifier

BacDive ID: 6099

Type strain: No

Species: Helicobacter pylori

Strain Designation: T81213-NTB, T81123-NTB

Strain history: CIP <- 1997, CCUG, Helicobacter nemestrinae <- 1993, ATCC <- M.A. Bronsdon: strain T81123-NTB

NCBI tax ID(s): 210 (species)

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General

@ref: 3190

BacDive-ID: 6099

DSM-Number: 7492

keywords: 16S sequence, Bacteria, mesophilic, Gram-negative, motile, rod-shaped

description: Helicobacter pylori T81213-NTB is a mesophilic, Gram-negative, motile bacterium that was isolated from gastric mucosa of pigtailed macaque.

NCBI tax id

  • NCBI tax id: 210
  • Matching level: species

strain history

@refhistory
3190<- ATCC; ATCC 49396 <- M. A. Bronsdon; T81213-NTB (Helicobacter nemestrinae)
67770CIP 104754 <-- CCUG 45423 <-- ATCC 49396 <-- M. A. Bronsdon T81213-NTB.
122083CIP <- 1997, CCUG, Helicobacter nemestrinae <- 1993, ATCC <- M.A. Bronsdon: strain T81123-NTB

doi: 10.13145/bacdive6099.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Epsilonproteobacteria
  • order: Campylobacterales
  • family: Helicobacteraceae
  • genus: Helicobacter
  • species: Helicobacter pylori
  • full scientific name: Helicobacter pylori (Marshall et al. 1985) Goodwin et al. 1989

  • synonyms

    @refsynonym
    20215Campylobacter pyloridis
    20215Helicobacter nemestrinae
    20215Campylobacter pylori

@ref: 3190

domain: Bacteria

phylum: Proteobacteria

class: Epsilonproteobacteria

order: Campylobacterales

family: Helicobacteraceae

genus: Helicobacter

species: Helicobacter pylori

full scientific name: Helicobacter pylori (Marshall et al. 1985) Goodwin et al. 1989

strain designation: T81213-NTB, T81123-NTB

type strain: no

Morphology

cell morphology

  • @ref: 122083
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 122083

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3190COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
39088MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
122083Brucella brothyes
122083CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
3190positivegrowth37mesophilic
39088positivegrowth37mesophilic
67770positivegrowth37mesophilic
122083positivegrowth30-41
122083nogrowth5psychrophilic
122083nogrowth10psychrophilic
122083nogrowth25mesophilic
122083nogrowth45thermophilic

Physiology and metabolism

halophily

  • @ref: 122083
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 3.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
122083606565hippurate-hydrolysis
12208317632nitrate-reduction
12208316301nitrite-reduction
6837316199urea+hydrolysis
6837317632nitrate-reduction
68373606565hippurate-hydrolysis
6837317634D-glucose-assimilation
6837330089acetate-assimilation
6837317272propionate-assimilation
6837325115malate-assimilation
6837316947citrate-assimilation

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistant
6837348923erythromycinyesyes14 µg
68373474053cefazolinyesyes224 µg
68373100147nalidixic acidyesyes84 µg
1220830129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)yesnoyes

metabolite production

@refChebi-IDmetaboliteproduction
6837316136hydrogen sulfideno
12208335581indoleno

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68373catalase+1.11.1.6
68373alkaline phosphatase+3.1.3.1
68373L-aspartate arylamidase-3.4.11.21
68373pyrrolidonyl arylamidase-3.4.19.3
68373gamma-glutamyltransferase+2.3.2.2
68373urease+3.5.1.5
122083oxidase+
122083gelatinase-
122083catalase+1.11.1.6
122083gamma-glutamyltransferase+2.3.2.2
122083urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122083-+++-+----++---+----

API CAM

@refURENITESTHIPGGTTTCPYRAArgAAspAPALH2SGLUSUTNALCFZACEPROPMLTCITEROCAT
3190+---+----+--+-------+
3190+---+----+----------+
3190+-+-+----+----------+
3190+---+----+----------+
3190+---+----+----------+
3190+-+-++-+-+----------+
3190+-+/--+----+----------+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudehost species
3190gastric mucosa of pigtailed macaqueSeattleUSAUSANorth America47.6062-122.332
67770Gastric mucosa of a 6-year-old healthy pigtailed macaque (Macaca nemestrina)Macaca nemestrina
122083Animal, Pig - tailed macaque, gastric mucosaUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Primates
#Host Body-Site#Gastrointestinal tract#Stomach

taxonmaps

  • @ref: 69479
  • File name: preview.99_91.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_36;96_72;97_75;98_82;99_91&stattab=map
  • Last taxonomy: Helicobacter pylori subclade
  • 16S sequence: LC507449
  • Sequence Identity:
  • Total samples: 18084
  • soil counts: 670
  • aquatic counts: 829
  • animal counts: 16307
  • plant counts: 278

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
31902Risk group (German classification)
1220832Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Helicobacter nemestrinae 16S ribosomal RNA gene, partial sequenceAF3486171477ena210
20218Helicobacter nemestrinae strain ATCC49396 16S ribosomal RNA gene, partial sequenceAF3630641437ena210
20218H.nemestrinae (ATCC 49396T) gene for 16S rRNAX678541463ena210
67770Helicobacter pylori JCM 12096 gene for 16S ribosomal RNA, partial sequenceLC5074491426ena210

GC content

@refGC-contentmethod
319024.0
6777024thermal denaturation, midpoint method (Tm)

External links

@ref: 3190

culture collection no.: DSM 7492, ATCC 49396, JCM 12096, CCUG 45423, CIP 104754, LMG 14378, NCTC 12491, CCUG 32350, CCUG 44615

straininfo link

  • @ref: 75587
  • straininfo: 7930

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny1995031Helicobacter nemestrinae sp. nov., a spiral bacterium found in the stomach of a pigtailed macaque (Macaca nemestrina)Bronsdon MA, Goodwin CS, Sly LI, Chilvers T, Schoenknecht FDInt J Syst Bacteriol10.1099/00207713-41-1-1481991Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, Gastric Mucosa/*microbiology, Gram-Negative Anaerobic Bacteria/*classification/cytology/isolation & purification/physiology, Macaca nemestrina/*microbiology, Terminology as TopicEnzymology
Phylogeny11931154Helicobacter nemestrinae ATCC 49396T is a strain of Helicobacter pylori (Marshall et al. 1985) Goodwin et al. 1989, and Helicobacter nemestrinae Bronsdon et al. 1991 is therefore a junior heterotypic synonym of Helicobacter pylori.Suerbaum S, Kraft C, Dewhirst FE, Fox JGInt J Syst Evol Microbiol10.1099/00207713-52-2-4372002Animals, DNA, Bacterial/chemistry, DNA, Ribosomal/chemistry, Flagellin/genetics, Genes, Bacterial, Helicobacter/*classification/genetics, Helicobacter pylori/classification, Macaca nemestrina/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/chemistry, Sequence Analysis, Species SpecificityGenetics
Enzymology31199937Development of a loop-mediated isothermal amplification assay for rapid Helicobacter pylori detection.Horiuchi S, Nakano R, Nakano A, Hishiya N, Uno K, Suzuki Y, Tanouchi A, Kakuta N, Masui T, Jojima N, Yano HJ Microbiol Methods10.1016/j.mimet.2019.1056532019Antigens, Bacterial/genetics, Bacterial Proteins/genetics, Feces/microbiology, Genes, Bacterial, Helicobacter Infections/*diagnosis, Helicobacter pylori/genetics/*isolation & purification, *Molecular Diagnostic Techniques/methods, *Nucleic Acid Amplification Techniques/methodsPhylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3190Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7492)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7492
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39088Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16697
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68373Automatically annotated from API CAM
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75587Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID7930.1StrainInfo: A central database for resolving microbial strain identifiers
122083Curators of the CIPCollection of Institut Pasteur (CIP 104754)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104754