General

@ref: 4931

BacDive-ID: 6093

DSM-Number: 13162

keywords: 16S sequence, Bacteria

description: Halothiobacillus kellyi Milos-BII1 is a bacterium that was isolated from marine shallow-water hydrothermal vent.

NCBI tax id

• NCBI tax id: 109263
• Matching level: species

strain history

• @ref: 4931
• history: <- J. Küver, Max-Planck-Inst. Marine Microbiology, Bremen, Germany; Milos-BII1 <- S. M. Sievert {1996}

doi: 10.13145/bacdive6093.20240510.9

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/pseudomonadota
• domain: Bacteria
• phylum: Pseudomonadota
• class: Gammaproteobacteria
• order: Chromatiales
• family: Halothiobacillaceae
• genus: Halothiobacillus
• species: Halothiobacillus kellyi
• full scientific name: Halothiobacillus kellyi Sievert et al. 2000

@ref: 4931

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Chromatiales

family: Halothiobacillaceae

genus: Halothiobacillus

species: Halothiobacillus kellyi

full scientific name: Halothiobacillus kellyi Sievert et al. 2000

strain designation: Milos-BII1

type strain: yes

Culture and growth conditions

culture medium

• @ref: 4931
• name: HALOTHIOBACILLUS MEDIUM (DSMZ Medium 864)
• growth: yes
• link: https://mediadive.dsmz.de/medium/864
• composition: Name: HALOTHIOBACILLUS MEDIUM (DSMZ Medium 864) Composition: NaCl 28.8557 g/l Na2S2O3 x 5 H2O 4.97512 g/l MgSO4 x 7 H2O 1.49254 g/l (NH4)2SO4 0.995025 g/l KCl 0.696517 g/l K2HPO4 0.497512 g/l CaCl2 x 2 H2O 0.41791 g/l Na2-EDTA x 2 H2O 0.00517413 g/l Bromothymol blue 0.0039801 g/l FeCl2 x 4 H2O 0.00149254 g/l CoCl2 x 6 H2O 0.000189055 g/l MnCl2 x 4 H2O 9.95025e-05 g/l ZnCl2 6.96517e-05 g/l Na2MoO4 x 2 H2O 3.58209e-05 g/l NiCl2 x 6 H2O 2.38806e-05 g/l H3BO3 5.97015e-06 g/l CuCl2 x 2 H2O 1.99005e-06 g/l Distilled water

culture temp

• @ref: 4931
• growth: positive
• type: growth
• temperature: 37

Isolation, sampling and environmental information

isolation

• @ref: 4931
• sample type: marine shallow-water hydrothermal vent
• geographic location: Milos Island
• country: Greece
• origin.country: GRC
• continent: Europe

isolation source categories

Safety information

risk assessment

• @ref: 4931
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 4931
• description: Halothiobacillus kellyi 16S ribosomal RNA gene, partial sequence
• accession: AF170419
• length: 1494
• database: nuccore
• NCBI tax ID: 109263

GC content

• @ref: 4931
• GC-content: 62

External links

@ref: 4931

culture collection no.: DSM 13162

straininfo link

• @ref: 75581
• straininfo: 49422

literature

• topic: Phylogeny
• Pubmed-ID: 10843067
• title: Halothiobacillus kellyi sp. nov., a mesophilic, obligately chemolithoautotrophic, sulfur-oxidizing bacterium isolated from a shallow-water hydrothermal vent in the Aegean Sea, and emended description of the genus Halothiobacillus.
• authors: Sievert SM, Heidorn T, Kuever J
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/00207713-50-3-1229
• year: 2000
• mesh: Culture Media, DNA, Bacterial/chemistry/genetics, Gammaproteobacteria/*classification/genetics/*isolation & purification/metabolism, Genes, rRNA, Geologic Sediments/*microbiology, Gram-Negative Chemolithotrophic Bacteria/*classification/genetics/growth & development/*isolation & purification, Molecular Sequence Data, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Sulfur/*metabolism, Thiobacillus/classification/genetics, Thiosulfates/metabolism
• topic2: Metabolism

Reference