Strain identifier

BacDive ID: 6091

Type strain: Yes

Species: Guyparkeria halophila

Strain Designation: 204

Strain history: <- A. P. Wood, King's College London, United Kingdom; 204

NCBI tax ID(s): 47960 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 2498

BacDive-ID: 6091

DSM-Number: 6132

keywords: 16S sequence, Bacteria, chemoautolithotroph, Gram-negative, motile, rod-shaped, colony-forming

description: Guyparkeria halophila 204 is a chemoautolithotroph, Gram-negative, motile bacterium that forms circular colonies and was isolated from lake O'Grady.

NCBI tax id

  • NCBI tax id: 47960
  • Matching level: species

strain history

  • @ref: 2498
  • history: <- A. P. Wood, King's College London, United Kingdom; 204

doi: 10.13145/bacdive6091.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Chromatiales
  • family: Thioalkalibacteraceae
  • genus: Guyparkeria
  • species: Guyparkeria halophila
  • full scientific name: Guyparkeria halophila (Wood and Kelly 1995) Boden 2017
  • synonyms

    @refsynonym
    20215Halothiobacillus halophilus
    20215Thiobacillus halophilus

@ref: 2498

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Chromatiales

family: Thioalkalibacteraceae

genus: Guyparkeria

species: Guyparkeria halophila

full scientific name: Guyparkeria halophila (Wood and Kelly 1995) Boden 2017

strain designation: 204

type strain: yes

Morphology

cell morphology

  • @ref: 43579
  • gram stain: negative
  • cell length: 1.0-1.2 µm
  • cell width: 0.3-0.5 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: polar

colony morphology

  • @ref: 43579
  • colony size: 1-3 mm
  • colony color: off-white
  • colony shape: circular
  • medium used: Basal salts agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2498GUYPARKERIA MEDIUM (DSMZ Medium 518)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium518.pdf
43579Basal salts agaryessupplemented with thiosulfate

culture temp

@refgrowthtypetemperature
2498positivegrowth30
43579positivegrowth26-36
43579positiveoptimum30-32

culture pH

  • @ref: 43579
  • ability: positive
  • type: optimum
  • pH: 7.0-7.3

Physiology and metabolism

nutrition type

  • @ref: 43579
  • type: chemoautolithotroph

spore formation

  • @ref: 43579
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentrationhalophily level
43579NaClpositivemaximum4 M
43579NaClpositiveoptimum1 Mhalophilic
43579NaClpositiveoptimum5.8 %(w/v)
43579NaClpositivemaximum23.2 %(w/v)

observation

  • @ref: 43579
  • observation: quinone UQ-8

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4357918022thiocyanate-nitrogen source
4357933403elemental sulfur+electron donor
4357915138sulfide+electron donor
4357915226tetrathionate+electron donor
4357915987trithionate+electron donor
4357931206ammonium chloride+nitrogen source
4357916094thiosulfate+nitrogen source
4357917632nitrate+reduction

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
2498lake O'GradyWestern AustraliaAustraliaAUSAustralia and Oceania
43579water from hypersaline playa Lake O´Grady, Shire of Koorda, Western AustraliaLake O´Grady, Shire of KoordaAustraliaAUSAustralia and Oceania

isolation source categories

Cat1Cat2Cat3
#Condition#Saline
#Environmental#Aquatic#Lake (large)

Safety information

risk assessment

  • @ref: 2498
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 2498
  • description: Guyparkeria halophila strain DSM 6132 16S ribosomal RNA, partial sequence
  • accession: NR_026015
  • length: 1504
  • database: nuccore
  • NCBI tax ID: 47960

GC content

@refGC-contentmethod
249845.0
4357964.2genome sequence analysis
249864.2high performance liquid chromatography (HPLC)

External links

@ref: 2498

culture collection no.: DSM 6132, ATCC 49870

straininfo link

  • @ref: 75579
  • straininfo: 42726

literature

  • topic: Metabolism
  • Pubmed-ID: 25613548
  • title: Formate supplementation can increase nickel recovery by Halothiobacillus halophilus.
  • authors: Vainshtein M, Abashina T, Bykov A, Repina A, Kaparullina E
  • journal: World J Microbiol Biotechnol
  • DOI: 10.1007/s11274-015-1805-6
  • year: 2015
  • mesh: Culture Media/*chemistry, Formates/*metabolism, Halothiobacillus/growth & development/*metabolism, Hydrogen-Ion Concentration, Nickel/*metabolism, Sulfides/*metabolism
  • topic2: Cultivation

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2498Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6132)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6132
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
43579Rich Boden10.1099/ijsem.0.002222Reclassification of Halothiobacillus hydrothermalis and Halothiobacillus halophilus to Guyparkeria gen. nov. in the Thioalkalibacteraceae fam. nov., with emended descriptions of the genus Halothiobacillus and family HalothiobacillaceaeIJSEM 67: 3919-3928 201728884673
75579Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42726.1StrainInfo: A central database for resolving microbial strain identifiers