Strain identifier
BacDive ID: 6085
Type strain: 
Species: Kushneria sinocarnis
Strain Designation: Z35
Strain history: <- G. Wang, Huazhong Agricultural Univ., Wuhan, China; Z35 <- Z. Zou
NCBI tax ID(s): 595502 (species)
version 8.1 (current version)
General
@ref: 16759
BacDive-ID: 6085
DSM-Number: 23229
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped
description: Kushneria sinocarnis Z35 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from Chinese traditional cured meat.
NCBI tax id
- NCBI tax id: 595502
- Matching level: species
strain history
- @ref: 16759
- history: <- G. Wang, Huazhong Agricultural Univ., Wuhan, China; Z35 <- Z. Zou
doi: 10.13145/bacdive6085.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Oceanospirillales
- family: Halomonadaceae
- genus: Kushneria
- species: Kushneria sinocarnis
- full scientific name: Kushneria sinocarnis Zou and Wang 2010
@ref: 16759
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Oceanospirillales
family: Halomonadaceae
genus: Kushneria
species: Kushneria sinocarnis
full scientific name: Kushneria sinocarnis Zou and Wang 2010
strain designation: Z35
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell length | cell width | cell shape | motility | confidence |
|---|---|---|---|---|---|---|
| 29472 | negative | 1.4-3.5 µm | 0.5-0.8 µm | rod-shaped | no | |
| 69480 | yes | 97.034 | ||||
| 69480 | negative | 99.99 |
pigmentation
- @ref: 29472
- production: yes
Culture and growth conditions
culture medium
- @ref: 16759
- name: MEDIUM 514 PLUS ADDITIONAL SALT (DSMZ Medium 514b)
- growth: yes
- link: https://mediadive.dsmz.de/medium/514b
- composition: Name: MEDIUM 514 plus additional salt (DSMZ Medium 514b; with strain-specific modifications) Composition: NaCl 50.0 g/l Agar 17.5 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 16759 | positive | growth | 37 | mesophilic |
| 29472 | positive | growth | 04-42 | |
| 29472 | positive | optimum | 37 | mesophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 29472 | positive | growth | 4.5-8.5 | alkaliphile |
| 29472 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
- @ref: 29472
- oxygen tolerance: aerobe
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.975 |
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 29472 | NaCl | positive | growth | 01-20 % |
| 29472 | NaCl | positive | optimum | 10 % |
observation
- @ref: 29472
- observation: aggregates in chains
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 29472 | 22599 | arabinose | + | carbon source |
| 29472 | 16947 | citrate | + | carbon source |
| 29472 | 28757 | fructose | + | carbon source |
| 29472 | 28260 | galactose | + | carbon source |
| 29472 | 24265 | gluconate | + | carbon source |
| 29472 | 17234 | glucose | + | carbon source |
| 29472 | 29987 | glutamate | + | carbon source |
| 29472 | 17754 | glycerol | + | carbon source |
| 29472 | 24996 | lactate | + | carbon source |
| 29472 | 17306 | maltose | + | carbon source |
| 29472 | 37684 | mannose | + | carbon source |
| 29472 | 28053 | melibiose | + | carbon source |
| 29472 | 26271 | proline | + | carbon source |
| 29472 | 15361 | pyruvate | + | carbon source |
| 29472 | 33942 | ribose | + | carbon source |
| 29472 | 17822 | serine | + | carbon source |
| 29472 | 30911 | sorbitol | + | carbon source |
| 29472 | 30031 | succinate | + | carbon source |
| 29472 | 17992 | sucrose | + | carbon source |
| 29472 | 27082 | trehalose | + | carbon source |
| 29472 | 18222 | xylose | + | carbon source |
| 29472 | 4853 | esculin | + | hydrolysis |
| 29472 | 17632 | nitrate | + | reduction |
enzymes
- @ref: 29472
- value: catalase
- activity: +
- ec: 1.11.1.6
Isolation, sampling and environmental information
isolation
- @ref: 16759
- sample type: Chinese traditional cured meat
- geographic location: Hubei Province, Wuhan
- country: China
- origin.country: CHN
- continent: Asia
isolation source categories
- Cat1: #Engineered
- Cat2: #Food production
- Cat3: #Meat
taxonmaps
- @ref: 69479
- File name: preview.99_134225.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_48;96_3068;97_3768;98_4784;99_134225&stattab=map
- Last taxonomy: Kushneria sinocarnis
- 16S sequence: FJ667549
- Sequence Identity:
- Total samples: 23
- aquatic counts: 7
- animal counts: 14
- plant counts: 2
Safety information
risk assessment
- @ref: 16759
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 16759
- description: Kushneria sinocarnis strain Z35 16S ribosomal RNA gene, partial sequence
- accession: FJ667549
- length: 1497
- database: ena
- NCBI tax ID: 595502
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Kushneria sinocarnis DSM 23229 | GCA_003633775 | contig | ncbi | 595502 |
| 66792 | Kushneria sinocarnis strain DSM 23229 | 595502.3 | wgs | patric | 595502 |
| 66792 | Kushneria sinocarnis DSM 23229 | 2757320357 | draft | img | 595502 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 16759 | 59.1 | high performance liquid chromatography (HPLC) |
| 29472 | 59.1 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| gram-positive | no | 98.003 | yes |
| anaerobic | no | 98.345 | yes |
| halophile | yes | 89.907 | yes |
| spore-forming | no | 95.2 | no |
| glucose-util | yes | 96.505 | yes |
| thermophile | no | 98.486 | yes |
| flagellated | no | 62.682 | no |
| aerobic | yes | 90.97 | yes |
| motile | yes | 80.626 | yes |
| glucose-ferment | no | 54.919 | no |
External links
@ref: 16759
culture collection no.: DSM 23229, CCTCC AB 209027, NRRL B-59197
straininfo link
- @ref: 75573
- straininfo: 403881
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 19783616 | Kushneria sinocarnis sp. nov., a moderately halophilic bacterium isolated from a Chinese traditional cured meat. | Zou Z, Wang G | Int J Syst Evol Microbiol | 10.1099/ijs.0.013797-0 | 2009 | DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Halomonadaceae/*classification/genetics/*isolation & purification/metabolism, Meat Products/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sodium Chloride/*metabolism | Metabolism |
| Phylogeny | 33355529 | Kushneria phosphatilytica sp. nov., a phosphate-solubilizing bacterium isolated from a solar saltern. | Du GX, Qu LY, Hong XG, Li CH, Ding DW, Gao P, Xu QZ | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004619 | 2021 | Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Halomonadaceae/*classification, Phosphates/*metabolism, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 23S/genetics, Seawater/*microbiology, Sequence Analysis, DNA | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 16759 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23229) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-23229 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 29472 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 25874 | 28776041 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 75573 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID403881.1 | StrainInfo: A central database for resolving microbial strain identifiers |