Strain identifier

BacDive ID: 6084

Type strain: Yes

Species: Kushneria aurantia

Strain Designation: A10

Strain history: A. Ventosa; Univ. of Sevilla, Spain; A10.

NCBI tax ID(s): 1122139 (strain), 504092 (species)

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General

@ref: 15635

BacDive-ID: 6084

DSM-Number: 21353

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Kushneria aurantia A10 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from surface of leaves of the black mangrove Avicennia germinans.

NCBI tax id

NCBI tax idMatching level
504092species
1122139strain

strain history

@refhistory
15635<- A. Ventosa, Univ. Seville, Dept. Microbiol. Parasitol., Spain <- C. Sánchez-Porro <- R. Montalvo-Rodríguez; A10
67770A. Ventosa; Univ. of Sevilla, Spain; A10.

doi: 10.13145/bacdive6084.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Oceanospirillales
  • family: Halomonadaceae
  • genus: Kushneria
  • species: Kushneria aurantia
  • full scientific name: Kushneria aurantia Sánchez-Porro et al. 2009

@ref: 15635

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Halomonadaceae

genus: Kushneria

species: Kushneria aurantia

full scientific name: Kushneria aurantia Sánchez-Porro et al. 2009

strain designation: A10

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
28913negative3.5 µm1 µmrod-shapedyes
69480yes98.041
69480negative99.847

pigmentation

  • @ref: 28913
  • production: yes

multimedia

@refmultimedia contentcaptionintellectual property rights
66793EM_DSM_21353_1.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_21353_2.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_21353_3.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_21353_4.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_21353_5.jpgelectron microscopic image© HZI/Manfred Rohde

Culture and growth conditions

culture medium

  • @ref: 15635
  • name: KUSHNERIA AURANTIA MEDIUM (DSMZ Medium 1195)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1195
  • composition: Name: KUSHNERIA AURANTIA MEDIUM (DSMZ Medium 1195) Composition: NaCl 78.0 g/l MgSO4 x 7 H2O 20.3 g/l Agar 20.0 g/l MgCl2 x 6 H2O 13.0 g/l Yeast extract 5.0 g/l KCl 2.0 g/l CaCl2 x 2 H2O 0.33 g/l NaBr 0.23 g/l NaHCO3 0.06 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
15635positivegrowth37mesophilic
28913positivegrowth20-40
28913positiveoptimum37mesophilic
67770positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
28913positivegrowth5.5-8.5alkaliphile
28913positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 28913
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
28913no
69481no100
69480no99.963

halophily

@refsaltgrowthtested relationconcentration
28913NaClpositivegrowth5-17.5 %
28913NaClpositiveoptimum10 %

observation

  • @ref: 67770
  • observation: quinones: Q-9

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
28913620642,3-butanediol+carbon source
2891315963ribitol+carbon source
2891322599arabinose+carbon source
2891317057cellobiose+carbon source
2891316947citrate+carbon source
2891323652dextrin+carbon source
2891328757fructose+carbon source
2891328260galactose+carbon source
2891324175galacturonate+carbon source
2891317234glucose+carbon source
2891329987glutamate+carbon source
2891317754glycerol+carbon source
2891328087glycogen+carbon source
2891317596inosine+carbon source
2891325115malate+carbon source
2891317306maltose+carbon source
2891329864mannitol+carbon source
2891337684mannose+carbon source
2891326271proline+carbon source
2891333942ribose+carbon source
2891317822serine+carbon source
2891330911sorbitol+carbon source
2891330031succinate+carbon source
2891326986threonine+carbon source
2891317748thymidine+carbon source
2891327082trehalose+carbon source
2891316704uridine+carbon source
2891317151xylitol+carbon source
2891318222xylose+carbon source
289134853esculin+hydrolysis

enzymes

  • @ref: 28913
  • value: catalase
  • activity: +
  • ec: 1.11.1.6

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
15635surface of leaves of the black mangrove Avicennia germinansAvicennia germinansCabo RojoPuerto RicoPRIMiddle and South America
67770Surface of leaves of the black mangrove Avicennia germinans tree growing near the solar salterns of Cabo RojoAvicennia germinansPuerto RicoUSAUSANorth America

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Mangrove
#Host#Plants#Tree
#Host Body-Site#Plant#Leaf (Phyllosphere)

taxonmaps

  • @ref: 69479
  • File name: preview.99_112657.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_48;96_44940;97_57591;98_76352;99_112657&stattab=map
  • Last taxonomy: Kushneria aurantia subclade
  • 16S sequence: AM941746
  • Sequence Identity:
  • Total samples: 178
  • soil counts: 8
  • aquatic counts: 92
  • animal counts: 74
  • plant counts: 4

Safety information

risk assessment

  • @ref: 15635
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15635
  • description: Kushneria aurantia partial 16S rRNA gene, type strain A10T
  • accession: AM941746
  • length: 1428
  • database: ena
  • NCBI tax ID: 504092

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Kushneria aurantia DSM 213531122139.9wgspatric1122139
66792Kushneria aurantia DSM 213532521172634draftimg1122139
67770Kushneria aurantia DSM 21353GCA_000382245scaffoldncbi1122139

GC content

@refGC-contentmethod
2891361.7
6777061.7thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes89.12yes
flagellatedno67.989no
gram-positiveno97.763yes
anaerobicno98.108yes
halophileyes87.085yes
spore-formingno95.924yes
thermophileno99.04yes
glucose-utilyes92.678yes
aerobicyes88.341no
glucose-fermentno63.652no

External links

@ref: 15635

culture collection no.: DSM 21353, CCM 7415, CECT 7220, JCM 18992, JCM 15469

straininfo link

  • @ref: 75572
  • straininfo: 343779

literature

  • topic: Phylogeny
  • Pubmed-ID: 19196785
  • title: Description of Kushneria aurantia gen. nov., sp. nov., a novel member of the family Halomonadaceae, and a proposal for reclassification of Halomonas marisflavi as Kushneria marisflavi comb. nov., of Halomonas indalinina as Kushneria indalinina comb. nov. and of Halomonas avicenniae as Kushneria avicenniae comb. nov.
  • authors: Sanchez-Porro C, de la Haba RR, Soto-Ramirez N, Marquez MC, Montalvo-Rodriguez R, Ventosa A
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.001461-0
  • year: 2009
  • mesh: Avicennia/microbiology, Fatty Acids/analysis, Halomonadaceae/chemistry/*classification/genetics/isolation & purification, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Species Specificity
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
15635Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21353)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21353
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
28913Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2535228776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life28604660 35: 676-683 2017
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
75572Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID343779.1StrainInfo: A central database for resolving microbial strain identifiers