Strain identifier

BacDive ID: 6082

Type strain: Yes

Species: Kushneria marisflavi

Strain Designation: SW32

Strain history: CIP <- 2001, JCM <- Y-H. Park: strain SW32

NCBI tax ID(s): 157779 (species)

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General

@ref: 5872

BacDive-ID: 6082

DSM-Number: 15357

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, motile

description: Kushneria marisflavi SW32 is an obligate aerobe, mesophilic, motile bacterium that was isolated from water sample.

NCBI tax id

  • NCBI tax id: 157779
  • Matching level: species

strain history

@refhistory
5872<- JCM <- Y.-H. Park; SW32
67770Y.-H. Park SW32.
120831CIP <- 2001, JCM <- Y-H. Park: strain SW32

doi: 10.13145/bacdive6082.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Oceanospirillales
  • family: Halomonadaceae
  • genus: Kushneria
  • species: Kushneria marisflavi
  • full scientific name: Kushneria marisflavi (Yoon et al. 2001) Sánchez-Porro et al. 2009

  • synonyms

    @refsynonym
    20215Halomonas marisflavi
    20215Halomonas marisflavae

@ref: 5872

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Halomonadaceae

genus: Kushneria

species: Kushneria marisflavi

full scientific name: Kushneria marisflavi (Yoon et al. 2001) Sánchez-Porro et al. 2009

strain designation: SW32

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes98.021
6948099.983negative
120831yesnegativerod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5872BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
40649MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120831CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5872positivegrowth25mesophilic
40649positivegrowth25mesophilic
67770positivegrowth25mesophilic
120831positivegrowth15-30
120831nogrowth5psychrophilic
120831nogrowth37mesophilic
120831nogrowth41thermophilic
120831nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120831
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.959

halophily

  • @ref: 120831
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

observation

  • @ref: 67770
  • observation: quinones: Q-9

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12083115824D-fructose-degradation
12083117634D-glucose-degradation
12083117716lactose-degradation
12083117306maltose-degradation
12083116947citrate+carbon source
1208314853esculin+hydrolysis
120831606565hippurate-hydrolysis
12083117632nitrate-reduction
12083116301nitrite-reduction
12083115792malonate-assimilation
12083117632nitrate-respiration

metabolite production

  • @ref: 120831
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
120831oxidase-
120831beta-galactosidase+3.2.1.23
120831alcohol dehydrogenase-1.1.1.1
120831gelatinase-
120831amylase-
120831DNase-
120831caseinase-3.4.21.50
120831catalase+1.11.1.6
120831tween esterase-
120831gamma-glutamyltransferase+2.3.2.2
120831lecithinase-
120831lipase-
120831lysine decarboxylase-4.1.1.18
120831ornithine decarboxylase-4.1.1.17
120831phenylalanine ammonia-lyase+4.3.1.24
120831protease-
120831tryptophan deaminase-
120831urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120831-++--+---+++---++---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120831+++++--+-++------++++-----+++--+-+-+++-++-+++---++++-+++++--+----+-----+-+-+---++-+------+++--+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
5872water sampleKorea, Yellow Sea, Boryung CityRepublic of KoreaKORAsia
67770Water sample of the Yellow SeaBoryung CityRepublic of KoreaKORAsia
120831Environment, Water sampleYellow Sea, Boryeong, ChungnamRepublic of KoreaKORAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_35332.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_48;96_6462;97_7747;98_10197;99_35332&stattab=map
  • Last taxonomy: Kushneria
  • 16S sequence: AF251143
  • Sequence Identity:
  • Total samples: 86
  • soil counts: 6
  • aquatic counts: 23
  • animal counts: 51
  • plant counts: 6

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
58721Risk group (German classification)
1208311Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Halomonas marisflavi genes for 16S ribosomal RNA, internal transcribed spacer 1 and 23S ribosomal RNA, partial and complete sequence, clone: 1133AB3672361133ena157779
5872Halomonas marisflava 16S ribosomal RNA gene, partial sequenceAF2511431495ena157779

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Kushneria marisflavi SW32GCA_002157205completencbi157779
66792Kushneria marisflavi strain DSM 15357157779.6wgspatric157779
66792Kushneria marisflavi strain SW32157779.3completepatric157779
66792Kushneria marisflavi SW322751185713completeimg157779
66792Kushneria marisflavi DSM 153572756170257draftimg157779
67770Kushneria marisflavi DSM 15357GCA_003610515scaffoldncbi157779

GC content

  • @ref: 5872
  • GC-content: 59
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno67.995no
gram-positiveno97.94no
anaerobicno98.104no
aerobicyes93.783no
halophileyes88.484no
spore-formingno95.424no
thermophileno99.434no
glucose-utilyes95.191no
motileyes89.205no
glucose-fermentno52.263no

External links

@ref: 5872

culture collection no.: DSM 15357, CIP 107103, JCM 10873, KCCM 80003, CGMCC 1.3499

straininfo link

@refstraininfo
75569100676
75570312087

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11411685Halomonas marisflavae sp. nov., a halophilic bacterium isolated from the Yellow Sea in Korea.Yoon JH, Choi SH, Lee KC, Kho YH, Kang KH, Park YHInt J Syst Evol Microbiol10.1099/00207713-51-3-11712001DNA, Ribosomal/genetics, Halomonas/*classification/genetics/isolation & purification/ultrastructure, Korea, Microscopy, Electron, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/microbiology, Ubiquinone/metabolismGenetics
Phylogeny19196785Description of Kushneria aurantia gen. nov., sp. nov., a novel member of the family Halomonadaceae, and a proposal for reclassification of Halomonas marisflavi as Kushneria marisflavi comb. nov., of Halomonas indalinina as Kushneria indalinina comb. nov. and of Halomonas avicenniae as Kushneria avicenniae comb. nov.Sanchez-Porro C, de la Haba RR, Soto-Ramirez N, Marquez MC, Montalvo-Rodriguez R, Ventosa AInt J Syst Evol Microbiol10.1099/ijs.0.001461-02009Avicennia/microbiology, Fatty Acids/analysis, Halomonadaceae/chemistry/*classification/genetics/isolation & purification, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Species SpecificityGenetics
Phylogeny30010522Kushneria phyllosphaerae sp. nov. and Kushneria endophytica sp. nov., plant growth promoting endophytes isolated from the halophyte plant Arthrocnemum macrostachyum.Navarro-Torre S, Carro L, Rodriguez-Llorente ID, Pajuelo E, Caviedes MA, Igual JM, Redondo-Gomez S, Camacho M, Klenk HP, Montero-Calasanz MDCInt J Syst Evol Microbiol10.1099/ijsem.0.0028972018Bacterial Typing Techniques, Base Composition, Chenopodiaceae/*microbiology, DNA, Bacterial/genetics, Endophytes/classification/isolation & purification, Fatty Acids/chemistry, Halomonadaceae/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Salt-Tolerant Plants/*microbiology, Sequence Analysis, DNA, Spain, Ubiquinone/chemistryEnzymology
Genetics33250124Complete genome sequence of the halophile bacterium Kushneria marisflavi KCCM 80003(T), isolated from seawater in Korea.Yun JH, Bae JWMar Genomics10.1016/j.margen.2017.11.0022017

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5872Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15357)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15357
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40649Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19306
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
75569Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100676.1StrainInfo: A central database for resolving microbial strain identifiers
75570Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID312087.1StrainInfo: A central database for resolving microbial strain identifiers
120831Curators of the CIPCollection of Institut Pasteur (CIP 107103)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107103