Strain identifier

BacDive ID: 6076

Type strain: Yes

Species: Halomonas titanicae

Strain Designation: BH1

Strain history: C. Sánchez-Porro BH1.

NCBI tax ID(s): 664683 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 16593

BacDive-ID: 6076

DSM-Number: 22872

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Halomonas titanicae BH1 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from rusticles of the RMS Titanic wreck.

NCBI tax id

  • NCBI tax id: 664683
  • Matching level: species

strain history

@refhistory
16593<- C. Sánchez-Porro, Univ. Seville, Dept. Microbiol. Parasitol., Spain; BH1 <- H. Mann
67770C. Sánchez-Porro BH1.

doi: 10.13145/bacdive6076.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Oceanospirillales
  • family: Halomonadaceae
  • genus: Halomonas
  • species: Halomonas titanicae
  • full scientific name: Halomonas titanicae Mann et al. 2010
  • synonyms

    • @ref: 20215
    • synonym: Vreelandella titanicae

@ref: 16593

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Halomonadaceae

genus: Halomonas

species: Halomonas titanicae

full scientific name: Halomonas titanicae Mann et al. 2010

strain designation: BH1

type strain: yes

Morphology

cell morphology

  • @ref: 29777
  • gram stain: negative
  • cell length: 1.5-6 µm
  • cell width: 0.5-0.8 µm
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 29777
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 16593
  • name: MEDIUM 514 PLUS ADDITIONAL SALT (DSMZ Medium 514b)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/514b
  • composition: Name: MEDIUM 514 plus additional salt (DSMZ Medium 514b; with strain-specific modifications) Composition: NaCl 60.0 g/l Agar 17.5 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
16593positivegrowth37mesophilic
29777positivegrowth04-42
29777positiveoptimum30-37mesophilic
67770positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
29777positivegrowth5.5-9.5alkaliphile
29777positiveoptimum7-7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 29777
  • oxygen tolerance: aerobe

spore formation

  • @ref: 29777
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
29777NaClpositivegrowth0.5-25 %
29777NaClpositiveoptimum02-08 %

observation

@refobservation
29777aggregates in chains
67770quinones: Q-9

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2977730089acetate+carbon source
2977717057cellobiose+carbon source
2977716947citrate+carbon source
2977728757fructose+carbon source
2977728260galactose+carbon source
2977717234glucose+carbon source
2977717754glycerol+carbon source
2977717716lactose+carbon source
2977729864mannitol+carbon source
2977717272propionate+carbon source
2977716634raffinose+carbon source
2977730911sorbitol+carbon source
2977717992sucrose+carbon source
2977727082trehalose+carbon source
2977717151xylitol+carbon source
2977718222xylose+carbon source
297774853esculin+hydrolysis

enzymes

@refvalueactivityec
29777catalase+1.11.1.6
29777cytochrome oxidase+1.9.3.1

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
16593rusticles of the RMS Titanic wreckNova Scotia, HalifaxCanadaCANNorth America
67770Rusticle sample obtained from the RMS Titanic

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Industrial
  • Cat3: #Engineered product

taxonmaps

  • @ref: 69479
  • File name: preview.99_1813.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_48;96_104;97_1197;98_1433;99_1813&stattab=map
  • Last taxonomy: Halomonas
  • 16S sequence: FN433898
  • Sequence Identity:
  • Total samples: 4368
  • soil counts: 321
  • aquatic counts: 3268
  • animal counts: 696
  • plant counts: 83

Safety information

risk assessment

  • @ref: 16593
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16593
  • description: Halomonas sp. BH1 partial 16S rRNA gene, type strain BH1T
  • accession: FN433898
  • length: 1453
  • database: ena
  • NCBI tax ID: 1204738

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Halomonas titanicae BH1GCA_000336575contigncbi1204738
66792Halomonas titanicae BH11204738.3wgspatric1204738
66792Halomonas titanicae BH12571042251draftimg1204738

GC content

@refGC-contentmethod
1659360thermal denaturation, midpoint method (Tm)
2977760
6777060high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes90.839yes
gram-positiveno98.395yes
anaerobicno98.723yes
aerobicyes92.815yes
halophileyes87.186no
spore-formingno96.022yes
thermophileno99.667yes
glucose-utilyes94.273yes
flagellatedyes62.308no
glucose-fermentno72.198no

External links

@ref: 16593

culture collection no.: DSM 22872, ATCC BAA 1257, CECT 7585, JCM 16411, LMG 25388

straininfo link

  • @ref: 75563
  • straininfo: 45450

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny20061494Halomonas titanicae sp. nov., a halophilic bacterium isolated from the RMS Titanic.Sanchez-Porro C, Kaur B, Mann H, Ventosa AInt J Syst Evol Microbiol10.1099/ijs.0.020628-02010Atlantic Ocean, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Halomonas/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Ships, Ubiquinone/chemistryGenetics
Phylogeny26431725Halomonas salicampi sp. nov., a halotolerant and alkalitolerant bacterium isolated from a saltern soil.Lee JC, Kim YS, Yun BS, Whang KSInt J Syst Evol Microbiol10.1099/ijsem.0.0006502015Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Halomonas/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, *Salinity, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/chemistryGenetics
Phylogeny33651187Halomonas sedimenti sp. nov., a Halotolerant Bacterium Isolated from Deep-Sea Sediment of the Southwest Indian Ocean.Qiu X, Yu L, Cao X, Wu H, Xu G, Tang XCurr Microbiol10.1007/s00284-021-02425-92021Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, *Halomonas/genetics, Indian Ocean, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
16593Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22872)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22872
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29777Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2615728776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75563Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID45450.1StrainInfo: A central database for resolving microbial strain identifiers