Strain identifier
BacDive ID: 6076
Type strain:
Species: Halomonas titanicae
Strain Designation: BH1
Strain history: C. Sánchez-Porro BH1.
NCBI tax ID(s): 664683 (species)
General
@ref: 16593
BacDive-ID: 6076
DSM-Number: 22872
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Halomonas titanicae BH1 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from rusticles of the RMS Titanic wreck.
NCBI tax id
- NCBI tax id: 664683
- Matching level: species
strain history
@ref | history |
---|---|
16593 | <- C. Sánchez-Porro, Univ. Seville, Dept. Microbiol. Parasitol., Spain; BH1 <- H. Mann |
67770 | C. Sánchez-Porro BH1. |
doi: 10.13145/bacdive6076.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Oceanospirillales
- family: Halomonadaceae
- genus: Halomonas
- species: Halomonas titanicae
- full scientific name: Halomonas titanicae Mann et al. 2010
synonyms
- @ref: 20215
- synonym: Vreelandella titanicae
@ref: 16593
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Oceanospirillales
family: Halomonadaceae
genus: Halomonas
species: Halomonas titanicae
full scientific name: Halomonas titanicae Mann et al. 2010
strain designation: BH1
type strain: yes
Morphology
cell morphology
- @ref: 29777
- gram stain: negative
- cell length: 1.5-6 µm
- cell width: 0.5-0.8 µm
- cell shape: rod-shaped
- motility: yes
pigmentation
- @ref: 29777
- production: yes
Culture and growth conditions
culture medium
- @ref: 16593
- name: MEDIUM 514 PLUS ADDITIONAL SALT (DSMZ Medium 514b)
- growth: yes
- link: https://mediadive.dsmz.de/medium/514b
- composition: Name: MEDIUM 514 plus additional salt (DSMZ Medium 514b; with strain-specific modifications) Composition: NaCl 60.0 g/l Agar 17.5 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
16593 | positive | growth | 37 | mesophilic |
29777 | positive | growth | 04-42 | |
29777 | positive | optimum | 30-37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
29777 | positive | growth | 5.5-9.5 | alkaliphile |
29777 | positive | optimum | 7-7.5 |
Physiology and metabolism
oxygen tolerance
- @ref: 29777
- oxygen tolerance: aerobe
spore formation
- @ref: 29777
- spore formation: no
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
29777 | NaCl | positive | growth | 0.5-25 % |
29777 | NaCl | positive | optimum | 02-08 % |
observation
@ref | observation |
---|---|
29777 | aggregates in chains |
67770 | quinones: Q-9 |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
29777 | 30089 | acetate | + | carbon source |
29777 | 17057 | cellobiose | + | carbon source |
29777 | 16947 | citrate | + | carbon source |
29777 | 28757 | fructose | + | carbon source |
29777 | 28260 | galactose | + | carbon source |
29777 | 17234 | glucose | + | carbon source |
29777 | 17754 | glycerol | + | carbon source |
29777 | 17716 | lactose | + | carbon source |
29777 | 29864 | mannitol | + | carbon source |
29777 | 17272 | propionate | + | carbon source |
29777 | 16634 | raffinose | + | carbon source |
29777 | 30911 | sorbitol | + | carbon source |
29777 | 17992 | sucrose | + | carbon source |
29777 | 27082 | trehalose | + | carbon source |
29777 | 17151 | xylitol | + | carbon source |
29777 | 18222 | xylose | + | carbon source |
29777 | 4853 | esculin | + | hydrolysis |
enzymes
@ref | value | activity | ec |
---|---|---|---|
29777 | catalase | + | 1.11.1.6 |
29777 | cytochrome oxidase | + | 1.9.3.1 |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
16593 | rusticles of the RMS Titanic wreck | Nova Scotia, Halifax | Canada | CAN | North America |
67770 | Rusticle sample obtained from the RMS Titanic |
isolation source categories
- Cat1: #Engineered
- Cat2: #Industrial
- Cat3: #Engineered product
taxonmaps
- @ref: 69479
- File name: preview.99_1813.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_48;96_104;97_1197;98_1433;99_1813&stattab=map
- Last taxonomy: Halomonas
- 16S sequence: FN433898
- Sequence Identity:
- Total samples: 4368
- soil counts: 321
- aquatic counts: 3268
- animal counts: 696
- plant counts: 83
Safety information
risk assessment
- @ref: 16593
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 16593
- description: Halomonas sp. BH1 partial 16S rRNA gene, type strain BH1T
- accession: FN433898
- length: 1453
- database: ena
- NCBI tax ID: 1204738
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Halomonas titanicae BH1 | GCA_000336575 | contig | ncbi | 1204738 |
66792 | Halomonas titanicae BH1 | 1204738.3 | wgs | patric | 1204738 |
66792 | Halomonas titanicae BH1 | 2571042251 | draft | img | 1204738 |
GC content
@ref | GC-content | method |
---|---|---|
16593 | 60 | thermal denaturation, midpoint method (Tm) |
29777 | 60 | |
67770 | 60 | high performance liquid chromatography (HPLC) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | yes | 90.839 | yes |
gram-positive | no | 98.395 | yes |
anaerobic | no | 98.723 | yes |
aerobic | yes | 92.815 | yes |
halophile | yes | 87.186 | no |
spore-forming | no | 96.022 | yes |
thermophile | no | 99.667 | yes |
glucose-util | yes | 94.273 | yes |
flagellated | yes | 62.308 | no |
glucose-ferment | no | 72.198 | no |
External links
@ref: 16593
culture collection no.: DSM 22872, ATCC BAA 1257, CECT 7585, JCM 16411, LMG 25388
straininfo link
- @ref: 75563
- straininfo: 45450
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 20061494 | Halomonas titanicae sp. nov., a halophilic bacterium isolated from the RMS Titanic. | Sanchez-Porro C, Kaur B, Mann H, Ventosa A | Int J Syst Evol Microbiol | 10.1099/ijs.0.020628-0 | 2010 | Atlantic Ocean, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Halomonas/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Ships, Ubiquinone/chemistry | Genetics |
Phylogeny | 26431725 | Halomonas salicampi sp. nov., a halotolerant and alkalitolerant bacterium isolated from a saltern soil. | Lee JC, Kim YS, Yun BS, Whang KS | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000650 | 2015 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Halomonas/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, *Salinity, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/chemistry | Genetics |
Phylogeny | 33651187 | Halomonas sedimenti sp. nov., a Halotolerant Bacterium Isolated from Deep-Sea Sediment of the Southwest Indian Ocean. | Qiu X, Yu L, Cao X, Wu H, Xu G, Tang X | Curr Microbiol | 10.1007/s00284-021-02425-9 | 2021 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, *Halomonas/genetics, Indian Ocean, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
16593 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22872) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-22872 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
29777 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26157 | 28776041 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
75563 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID45450.1 | StrainInfo: A central database for resolving microbial strain identifiers |