Strain identifier

BacDive ID: 6033

Type strain: Yes

Species: Halomonas magadiensis

Strain Designation: 21M1, 21 MI

Strain history: CIP <- 2000, NCIMB <- W.D. Grant: strain 21 MI

NCBI tax ID(s): 150024 (species)

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General

@ref: 5881

BacDive-ID: 6033

DSM-Number: 15367

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative

description: Halomonas magadiensis 21M1 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from litoral sediments.

NCBI tax id

  • NCBI tax id: 150024
  • Matching level: species

strain history

@refhistory
5881<- NCIMB <- W. D. Grant, Univ. Leicester; 21M1
67771<- DSM <- NCIMB <- WD Grant, Univ. Leicester; 21M1
120746CIP <- 2000, NCIMB <- W.D. Grant: strain 21 MI

doi: 10.13145/bacdive6033.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Oceanospirillales
  • family: Halomonadaceae
  • genus: Halomonas
  • species: Halomonas magadiensis
  • full scientific name: Halomonas magadiensis corrig. Duckworth et al. 2000

  • synonyms

    • @ref: 20215
    • synonym: Halomonas magadii

@ref: 5881

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Halomonadaceae

genus: Halomonas

species: Halomonas magadiensis

full scientific name: Halomonas magadiensis Duckworth et al. 2000

strain designation: 21M1, 21 MI

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
67771negative
120746negativerod-shapedyes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
39592MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120746CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperature
39592positivegrowth37
67771positivegrowth37
120746positivegrowth15-37
120746nogrowth5
120746nogrowth41
120746nogrowth45

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
67771aerobe
120746obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
120746NaClpositivegrowth2-10 %
120746NaClnogrowth0 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1207464853esculin-hydrolysis
120746606565hippurate-hydrolysis
12074617632nitrate+reduction
12074616301nitrite-reduction
12074615792malonate-assimilation
12074617632nitrate-respiration
68371320061methyl alpha-D-glucopyranoside-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from

metabolite production

  • @ref: 120746
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
120746oxidase+
120746beta-galactosidase-3.2.1.23
120746alcohol dehydrogenase-1.1.1.1
120746gelatinase-
120746amylase-
120746caseinase-3.4.21.50
120746catalase+1.11.1.6
120746tween esterase-
120746gamma-glutamyltransferase+2.3.2.2
120746lecithinase-
120746lipase-
120746lysine decarboxylase-4.1.1.18
120746ornithine decarboxylase-4.1.1.17
120746phenylalanine ammonia-lyase+4.3.1.24
120746tryptophan deaminase-
120746urease+3.5.1.5
68382alkaline phosphatase-3.1.3.1

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120746--++-++----+---+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120746---+/---------------------------------------+/------+/-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120746++++---+-++---------+-+--++----+-++++---------++++++-++++---+-+++--------+-+---++++-----+++++++-+-+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5881litoral sedimentsLake MagadiiKenyaKENAfrica
67771From Litoral sediments; KenyaLake MagadiiKenyaKENAfrica
120746Environment, Littoral sediments, Magadii lakeKenyaKENAfrica1988

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Sediment

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
58811Risk group (German classification)
1207461Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5881
  • description: Bacterial sp. 16S rRNA gene (Lake Magadi isolate 21M1)
  • accession: X92150
  • length: 1473
  • database: nuccore
  • NCBI tax ID: 150024

External links

@ref: 5881

culture collection no.: DSM 15367, KCTC 22195, NCIMB 13595, CIP 106823, CIP 106874

straininfo link

  • @ref: 75519
  • straininfo: 93065

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10741837Halomonas magadii sp. nov., a new member of the genus Halomonas, isolated from a soda lake of the East African Rift Valley.Duckworth AW, Grant WD, Jones BE, Meijer D, Marquez MC, Ventosa AExtremophiles10.1007/s0079200500072000Africa, DNA/chemistry, DNA, Ribosomal/genetics, Halomonas/*classification/genetics/growth & development, Hydrogen-Ion Concentration, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Salts, Sequence Analysis, DNAPhenotype
Genetics12668114The O-specific chain structure of the major component from the lipopolysaccharide fraction of Halomonas magadii strain 21 MI (NCIMB 13595).de Castro C, Molinaro A, Nunziata R, Grant W, Wallace A, Parrilli MCarbohydr Res10.1016/s0008-6215(02)00502-52003Carbohydrate Sequence, Halomonas/*chemistry, Lipopolysaccharides/*chemistry, Magnetic Resonance Spectroscopy, Molecular Sequence Data
Phylogeny19651714Halomonas stevensii sp. nov., Halomonas hamiltonii sp. nov. and Halomonas johnsoniae sp. nov., isolated from a renal care centre.Kim KK, Lee KC, Oh HM, Lee JSInt J Syst Evol Microbiol10.1099/ijs.0.004424-02009Bacteremia/etiology/*microbiology, Base Composition, Base Sequence, California, DNA, Bacterial/chemistry, Equipment and Supplies, Hospital/*microbiology, Halomonas/*classification/genetics/isolation & purification, *Hemodialysis Units, Hospital, Humans, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Renal Dialysis/*instrumentation, Sequence Homology, Nucleic AcidPathogenicity

Reference

@idauthorscataloguedoi/urltitle
5881Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15367)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15367
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39592Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18996
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
75519Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID93065.1StrainInfo: A central database for resolving microbial strain identifiers
120746Curators of the CIPCollection of Institut Pasteur (CIP 106823)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106823