Strain identifier

BacDive ID: 5997

Type strain: Yes

Species: Chromohalobacter israelensis

Strain Designation: Ba1, Ba1, 1-13-31

Strain history: CIP <- 1998, R.H. Vreeland, West Chester Univ., USA: strain 1-13-31

NCBI tax ID(s): 141390 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 2807

BacDive-ID: 5997

DSM-Number: 6768

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped

description: Chromohalobacter israelensis Ba1 is an obligate aerobe, mesophilic, Gram-negative bacterium of the family Halomonadaceae.

NCBI tax id

  • NCBI tax id: 141390
  • Matching level: species

strain history

@refhistory
2807<- ATCC
120285CIP <- 1998, R.H. Vreeland, West Chester Univ., USA: strain 1-13-31

doi: 10.13145/bacdive5997.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Oceanospirillales
  • family: Halomonadaceae
  • genus: Chromohalobacter
  • species: Chromohalobacter israelensis
  • full scientific name: Chromohalobacter israelensis (Huval et al. 1996) Arahal et al. 2001
  • synonyms

    • @ref: 20215
    • synonym: Halomonas israelensis

@ref: 2807

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Halomonadaceae

genus: Chromohalobacter

species: Chromohalobacter israelensis

full scientific name: Chromohalobacter israelensis (Huval et al. 1996) Arahal et al. 2001

strain designation: Ba1, Ba1, 1-13-31

type strain: yes

Morphology

cell morphology

  • @ref: 120285
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
280710% MH MEDIUM (DSMZ Medium 593)yeshttps://mediadive.dsmz.de/medium/593Name: 10% MH MEDIUM (DSMZ Medium 593) Composition: NaCl 81.0 g/l Agar 15.0 g/l Yeast extract 10.0 g/l MgSO4 9.6 g/l MgCl2 7.0 g/l Proteose peptone no. 3 5.0 g/l KCl 2.0 g/l Glucose 1.0 g/l CaCl2 0.36 g/l NaHCO3 0.06 g/l NaBr 0.026 g/l Distilled water
39318Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
120285CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
2807positivegrowth37mesophilic
39318positivegrowth30mesophilic
120285positivegrowth15-41
120285nogrowth5psychrophilic
120285nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120285
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
120285NaClpositivegrowth6-10 %
120285NaClnogrowth0 %
120285NaClnogrowth2 %
120285NaClnogrowth4 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
120285esculin-hydrolysis4853
120285hippurate-hydrolysis606565
120285nitrate+reduction17632
120285nitrite-reduction16301
120285malonate-assimilation15792
120285nitrate-respiration17632

metabolite production

  • @ref: 120285
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
120285oxidase-
120285beta-galactosidase-3.2.1.23
120285alcohol dehydrogenase-1.1.1.1
120285gelatinase-
120285amylase-
120285caseinase-3.4.21.50
120285catalase+1.11.1.6
120285tween esterase-
120285gamma-glutamyltransferase+2.3.2.2
120285lecithinase-
120285lipase-
120285lysine decarboxylase-4.1.1.18
120285ornithine decarboxylase-4.1.1.17
120285phenylalanine ammonia-lyase-4.3.1.24
120285protease-
120285tryptophan deaminase-
120285urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120285-++--++---++---+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120285-------------------------------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120285+++-++-+-++--------+++----+-++-+-+-+++-++-+++---+++--++-+---+----------+-+-+---++-+-----+-+++++----

Isolation, sampling and environmental information

isolation

@refcountryorigin.countrycontinentsample type
39318IsraelISRAsia
2807IsraelISRAsiaDead Sea
120285IsraelISRAsiaEnvironment, Water, Dead sea

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic
#Environmental#Aquatic#Lake (large)
#Environmental#Aquatic#Marine
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_4319.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_48;96_1010;97_1176;98_3251;99_4319&stattab=map
  • Last taxonomy: Chromohalobacter
  • 16S sequence: AJ295144
  • Sequence Identity:
  • Total samples: 1588
  • soil counts: 65
  • aquatic counts: 1028
  • animal counts: 412
  • plant counts: 83

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
28071Risk group (German classification)
1202851Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Chromohalobacter israelensis 16S rRNA gene, strain ATCC 43985 TAJ2951441532ena141390
2807Chromohalobacter israelensis 16S ribosomal RNA gene, partial sequenceAF2118621492ena141390

Genome sequences

  • @ref: 66792
  • description: Chromohalobacter israelensis DSM 6768
  • accession: GCA_029478115
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 141390

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes93.066no
flagellatedno54.515no
gram-positiveno98.598no
anaerobicno99.389no
aerobicyes92.131no
halophileyes93.884no
spore-formingno96.769no
glucose-fermentno60.561no
thermophileno98.84yes
glucose-utilyes93.264no

External links

@ref: 2807

culture collection no.: DSM 6768, ATCC 43985, CIP 105572

straininfo link

  • @ref: 75486
  • straininfo: 13197

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny8542554Description of two new species of Halomonas: Halomonas israelensis sp.nov. and Halomonas canadensis sp.nov.Huval JH, Latta R, Wallace R, Kushner DJ, Vreeland RHCan J Microbiol10.1139/m95-1561995Culture Media/chemistry, Gram-Negative Aerobic Bacteria/*classification/enzymology/genetics/*growth & development, Microbial Sensitivity Tests, Sodium Chloride, Species SpecificityCultivation
Phylogeny11491344Transfer of Halomonas canadensis and Halomonas israelensis to the genus Chromohalobacter as Chromohalobacter canadensis comb. nov. and Chromohalobacter israelensis comb. nov..Arahal DR, Garcia MT, Ludwig W, Schleifer KH, Ventosa AInt J Syst Evol Microbiol10.1099/00207713-51-4-14432001DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Gammaproteobacteria/*classification/*genetics/metabolism, Halomonas/*classification/*genetics/metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species SpecificityMetabolism
Phylogeny25180007Larsenia salina gen. nov., sp. nov., a new member of the family Halomonadaceae based on multilocus sequence analysis.Leon MJ, Sanchez-Porro C, de la Haba RR, Llamas I, Ventosa ASyst Appl Microbiol10.1016/j.syapm.2014.07.0062014Aerobiosis, Bacterial Proteins/genetics, Bacterial Typing Techniques, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, *Environmental Microbiology, Fatty Acids/analysis, Halomonadaceae/*classification/*genetics/isolation & purification/physiology, Hydrogen-Ion Concentration, Locomotion, Molecular Sequence Data, *Multilocus Sequence Typing, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, Sodium Chloride/metabolism, Spain, TemperatureGenetics
Genetics25481275Investigation of mercury tolerance in Chromohalobacter israelensis DSM 6768T and Halomonas zincidurans B6T by comparative genomics with Halomonas xinjiangensis TRM 0175T.Zhou P, Huo YY, Xu L, Wu YH, Meng FX, Wang CS, Xu XWMar Genomics10.1016/j.margen.2014.11.0082014Atlantic Ocean, Base Sequence, Chromohalobacter/*drug effects/*genetics, Dose-Response Relationship, Drug, Drug Tolerance/*genetics, Halomonas/*drug effects/*genetics, Mercury/*toxicity, Microbial Sensitivity Tests, Molecular Sequence Annotation, Molecular Sequence Data, Sequence Analysis, DNA, Species SpecificityPathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2807Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6768)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6768
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39318Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17606
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75486Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13197.1StrainInfo: A central database for resolving microbial strain identifiers
120285Curators of the CIPCollection of Institut Pasteur (CIP 105572)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105572