Strain identifier

BacDive ID: 5996

Type strain: Yes

Species: Chromohalobacter canadensis

Strain Designation: 1-13-26

Strain history: CIP <- 1998, R.H. Vreeland, West Chester Univ., Pennsylvania, USA: strain 1-13-26 <- D.J. Kushner <- NRCC <- A.T. Matheson

NCBI tax ID(s): 141389 (species)

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General

@ref: 2808

BacDive-ID: 5996

DSM-Number: 6769

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, oval-shaped

description: Chromohalobacter canadensis 1-13-26 is an obligate aerobe, mesophilic, Gram-negative bacterium of the family Halomonadaceae.

NCBI tax id

  • NCBI tax id: 141389
  • Matching level: species

strain history

@refhistory
2808<- ATCC <- R. H. Vreeland, strain HX
117124CIP <- 1998, R.H. Vreeland, West Chester Univ., Pennsylvania, USA: strain 1-13-26 <- D.J. Kushner <- NRCC <- A.T. Matheson

doi: 10.13145/bacdive5996.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Oceanospirillales
  • family: Halomonadaceae
  • genus: Chromohalobacter
  • species: Chromohalobacter canadensis
  • full scientific name: Chromohalobacter canadensis (Huval et al. 1996) Arahal et al. 2001

  • synonyms

    • @ref: 20215
    • synonym: Halomonas canadensis

@ref: 2808

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Halomonadaceae

genus: Chromohalobacter

species: Chromohalobacter canadensis

full scientific name: Chromohalobacter canadensis (Huval et al. 1996) Arahal et al. 2001

strain designation: 1-13-26

type strain: yes

Morphology

cell morphology

  • @ref: 117124
  • gram stain: negative
  • cell shape: oval-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
280810% MH MEDIUM (DSMZ Medium 593)yeshttps://mediadive.dsmz.de/medium/593Name: 10% MH MEDIUM (DSMZ Medium 593) Composition: NaCl 81.0 g/l Agar 15.0 g/l Yeast extract 10.0 g/l MgSO4 9.6 g/l MgCl2 7.0 g/l Proteose peptone no. 3 5.0 g/l KCl 2.0 g/l Glucose 1.0 g/l CaCl2 0.36 g/l NaHCO3 0.06 g/l NaBr 0.026 g/l Distilled water
40200Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
117124CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
2808positivegrowth28mesophilic
40200positivegrowth30mesophilic
117124positivegrowth15-41
117124nogrowth5psychrophilic
117124nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 117124
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
117124NaClpositivegrowth2-10 %
117124NaClnogrowth0 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
117124esculin-hydrolysis4853
117124nitrate-builds gas from17632
117124nitrate+reduction17632
117124nitrite-builds gas from16301
117124nitrite-reduction16301
117124malonate-assimilation15792
117124glucose+/-degradation17234
117124nitrate-respiration17632

antibiotic resistance

  • @ref: 117124
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 117124
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11712415688acetoin-
11712417234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
117124oxidase-
117124beta-galactosidase-3.2.1.23
117124alcohol dehydrogenase-1.1.1.1
117124gelatinase-
117124amylase-
117124DNase-
117124caseinase-3.4.21.50
117124catalase+1.11.1.6
117124tween esterase-
117124gamma-glutamyltransferase-2.3.2.2
117124lecithinase-
117124lipase-
117124lysine decarboxylase-4.1.1.18
117124ornithine decarboxylase-4.1.1.17
117124phenylalanine ammonia-lyase-4.3.1.24
117124tryptophan deaminase-
117124urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
117124-++--+----++---+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
117124-------------------------------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
117124+++++-++++++++++++++++++-++++--+++++++--++-++---+++--++++--++-++-----+-+++-++++++--++---++++++++++-

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample typeisolation date
40200OttawaCanadaCANNorth America
117124OttawaCanadaCANNorth AmericaHalophilic medium contaminant1973

taxonmaps

  • @ref: 69479
  • File name: preview.99_1780.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_48;96_1010;97_1176;98_1406;99_1780&stattab=map
  • Last taxonomy: Chromohalobacter
  • 16S sequence: AJ295143
  • Sequence Identity:
  • Total samples: 165
  • soil counts: 8
  • aquatic counts: 74
  • animal counts: 45
  • plant counts: 38

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
28081Risk group (German classification)
1171241Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Chromohalobacter canadensis 16S rRNA gene, strain ATCC 43984 TAJ2951431532ena141389
2808Chromohalobacter canadensis 16S ribosomal RNA gene, partial sequenceAF2118611495ena141389

Genome sequences

  • @ref: 66792
  • description: Chromohalobacter canadensis DSM 6769
  • accession: GCA_023061155
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 141389

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedyes50.507no
gram-positiveno98.392no
anaerobicno99.139no
aerobicyes93.714no
halophileyes95.328no
spore-formingno96.45no
motileyes92.248no
glucose-fermentno54.345no
thermophileno98.074yes
glucose-utilyes95.095no

External links

@ref: 2808

culture collection no.: DSM 6769, ATCC 43984, NRCC 41227, CIP 105571

straininfo link

  • @ref: 75485
  • straininfo: 13198

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny8542554Description of two new species of Halomonas: Halomonas israelensis sp.nov. and Halomonas canadensis sp.nov.Huval JH, Latta R, Wallace R, Kushner DJ, Vreeland RHCan J Microbiol10.1139/m95-1561995Culture Media/chemistry, Gram-Negative Aerobic Bacteria/*classification/enzymology/genetics/*growth & development, Microbial Sensitivity Tests, Sodium Chloride, Species SpecificityCultivation
Phylogeny11491344Transfer of Halomonas canadensis and Halomonas israelensis to the genus Chromohalobacter as Chromohalobacter canadensis comb. nov. and Chromohalobacter israelensis comb. nov..Arahal DR, Garcia MT, Ludwig W, Schleifer KH, Ventosa AInt J Syst Evol Microbiol10.1099/00207713-51-4-14432001DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Gammaproteobacteria/*classification/*genetics/metabolism, Halomonas/*classification/*genetics/metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species SpecificityMetabolism
Phylogeny15545411Chromohalobacter sarecensis sp. nov., a psychrotolerant moderate halophile isolated from the saline Andean region of Bolivia.Quillaguaman J, Delgado O, Mattiasson B, Hatti-Kaul RInt J Syst Evol Microbiol10.1099/ijs.0.63153-02004Amino Acid Oxidoreductases/analysis, Bacterial Typing Techniques, Base Composition, Bolivia, Carbohydrate Metabolism, Catalase/analysis, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Genes, rRNA, Gentian Violet, Halomonadaceae/*classification/cytology/genetics/*isolation & purification/metabolism/physiology, Hydrogen Sulfide/metabolism, Hydrogen-Ion Concentration, Indoles/metabolism, L-Amino Acid Oxidase, Molecular Sequence Data, Movement, Nucleic Acid Hybridization, Oxidoreductases/analysis, Phenazines, Phylogeny, Pseudomonas/genetics, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Saline Solution, Hypertonic, Sequence Analysis, DNA, *Soil Microbiology, TemperatureEnzymology
Phylogeny17911293Chromohalobacter japonicus sp. nov., a moderately halophilic bacterium isolated from a Japanese salty food.Sanchez-Porro C, Tokunaga H, Tokunaga M, Ventosa AInt J Syst Evol Microbiol10.1099/ijs.0.65212-02007Bacterial Typing Techniques, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, *Food Microbiology, Genes, rRNA, Halomonadaceae/chemistry/*classification/*isolation & purification/physiology, Hydrogen-Ion Concentration, Japan, Locomotion/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sodium Chloride/metabolism, TemperatureBiotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2808Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6769)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6769
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40200Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17605
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75485Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13198.1StrainInfo: A central database for resolving microbial strain identifiers
117124Curators of the CIPCollection of Institut Pasteur (CIP 105571)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105571