General

@ref: 5052

BacDive-ID: 5991

DSM-Number: 13466

keywords: 16S sequence, Bacteria, anaerobe, mesophilic

description: Orenia salinaria DSM 13466 is an anaerobe, mesophilic bacterium that was isolated from saltern of Salin-de-Giraud.

NCBI tax id

• NCBI tax id: 85450
• Matching level: species

strain history

• @ref: 5052
• history: <- P. Caumette; SG 3902

doi: 10.13145/bacdive5991.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/bacillota
• domain: Bacteria
• phylum: Bacillota
• class: Clostridia
• order: Halanaerobiales
• family: Halobacteroidaceae
• genus: Orenia
• species: Orenia salinaria
• full scientific name: Orenia salinaria Mouné et al. 2000

@ref: 5052

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Halanaerobiales

family: Halobacteroidaceae

genus: Orenia

species: Orenia salinaria

full scientific name: Orenia salinaria Mouné et al. 2000

type strain: yes

Culture and growth conditions

culture medium

• @ref: 5052
• name: HALOANAEROBACTER SALINARIUS MEDIUM (DSMZ Medium 842)
• growth: yes
• link: https://mediadive.dsmz.de/medium/842
• composition: Name: HALOANAEROBACTER SALINARIUS MEDIUM (DSMZ Medium 842) Composition: NaCl 150.0 g/l MgCl2 x 6 H2O 15.0 g/l KCl 3.0 g/l NaHCO3 2.0 g/l Glucose 1.2 g/l Na2S x 9 H2O 0.5 g/l NH4Cl 0.5 g/l KH2PO4 0.35 g/l Yeast extract 0.1 g/l CaCl2 x 2 H2O 0.05 g/l Na2-EDTA 0.003 g/l FeSO4 x 7 H2O 0.0011 g/l Resazurin 0.001 g/l NaOH 0.0004 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.00019 g/l Thiamine 0.0001 g/l Vitamin B12 5e-05 g/l MnCl2 x 2 H2O 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l ZnCl2 4.2e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Na2MoO4 x 2 H2O 1.8e-05 g/l Biotin 1e-05 g/l Na2WO4 x 2 H2O 8e-06 g/l Na2SeO3 x 5 H2O 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water

culture temp

• @ref: 5052
• growth: positive
• type: growth
• temperature: 30
• range: mesophilic

Physiology and metabolism

oxygen tolerance

• @ref: 5052
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

• @ref: 5052
• sample type: saltern of Salin-de-Giraud
• geographic location: Rhone delta, Camargue
• country: France
• origin.country: FRA
• continent: Europe

isolation source categories

• Cat1: #Condition
• Cat2: #Saline

taxonmaps

• @ref: 69479
• File name: preview.99_104989.png
• url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15978;96_42284;97_54062;98_71465;99_104989&stattab=map
• Last taxonomy: Orenia salinaria subclade
• 16S sequence: Y18485
• Sequence Identity:
• Total samples: 1065
• soil counts: 166
• aquatic counts: 761
• animal counts: 112
• plant counts: 26

Safety information

risk assessment

• @ref: 5052
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 5052
• description: Orenia salinaria 16S rRNA gene
• accession: Y18485
• length: 1337
• database: ena
• NCBI tax ID: 85450

GC content

• @ref: 5052
• GC-content: 33.7
• method: high performance liquid chromatography (HPLC)

External links

@ref: 5052

culture collection no.: DSM 13466, ATCC 700911, SG 3902

straininfo link

• @ref: 75480
• straininfo: 45446

literature

• topic: Phylogeny
• Pubmed-ID: 10758881
• title: Orenia salinaria sp. nov., a fermentative bacterium isolated from anaerobic sediments of Mediterranean salterns.
• authors: Moune S, Eatock C, Matheron R, Willison JC, Hirschler A, Herbert R, Caumette P
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/00207713-50-2-721
• year: 2000
• mesh: Anaerobiosis, Base Composition, Culture Media, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Genes, rRNA, Geologic Sediments/*microbiology, Gram-Negative Anaerobic Straight, Curved, and Helical Rods/*classification/cytology/physiology, Mediterranean Region, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Sodium Chloride/pharmacology, *Water Microbiology
• topic2: Cultivation

Reference