Archive BacDiveID:10.13145/bacdive5982.20250331.9.3

Strain identifier

BacDive ID: 5982

Type strain:

Species: Halanaerobacter jeridensis

Strain Designation: CEJFG43

Strain history: D. Alazard CEJFG43.

NCBI tax ID(s): 706427 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 16760

BacDive-ID: 5982

DSM-Number: 23230

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, Gram-negative, motile

description: Halanaerobacter jeridensis CEJFG43 is an anaerobe, thermophilic, Gram-negative bacterium that was isolated from black sediment below the salt crust of a hypersaline lake.

NCBI tax id

NCBI tax id: 706427
Matching level: species

strain history

@ref	history
16760	<- D. Alazard, Lab. Microbiol. de l'IRD/ESIL, Univ. de Provence et de la Méditerranée, Marseille, France; CEJFG43
67770	D. Alazard CEJFG43.

doi: 10.13145/bacdive5982.20250331.9.3

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/bacillota
domain: Bacteria
phylum: Bacillota
class: Clostridia
order: Halanaerobiales
family: Halobacteroidaceae
genus: Halanaerobacter
species: Halanaerobacter jeridensis
full scientific name: Halanaerobacter jeridensis Mezghani et al. 2012

@ref: 16760

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Halanaerobiales

family: Halobacteroidaceae

genus: Halanaerobacter

species: Halanaerobacter jeridensis

full scientific name: Halanaerobacter jeridensis Mezghani et al. 2012

strain designation: CEJFG43

type strain: yes

Morphology

cell morphology

@ref: 30446
gram stain: negative
cell length: 4.25 µm
cell width: 1.2 µm
motility: yes

Culture and growth conditions

culture medium

@ref: 16760
name: HALANAEROBACTER JERIDENSE MEDIUM (DSMZ Medium 1375)
growth: yes
link: https://mediadive.dsmz.de/medium/1375
composition: Name: HALANAEROBACTER JERIDENSE MEDIUM (DSMZ Medium 1375) Composition: Na2CO3 25.3 g/l Glucose 15.44 g/l MgCl2 x 6 H2O 10.0 g/l KCl 2.0 g/l NH4Cl 1.0 g/l K2HPO4 x 3 H2O 0.5 g/l MgSO4 x 7 H2O 0.03 g/l Nitrilotriacetic acid 0.015 g/l NaCl 0.01 g/l MnSO4 x H2O 0.005 g/l ZnSO4 x 7 H2O 0.0018 g/l CoSO4 x 7 H2O 0.0018 g/l CaCl2 x 2 H2O 0.001 g/l Resazurin 0.001 g/l FeSO4 x 7 H2O 0.001 g/l NiCl2 x 6 H2O 0.0003 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l CuSO4 x 5 H2O 0.0001 g/l H3BO3 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l Na2SeO3 x 5 H2O 3e-06 g/l Na2S x 9 H2O Yeast extract L-Cysteine Distilled water

culture temp

@ref	growth	type	temperature
16760	positive	growth	40
30446	positive	growth	20-60
30446	positive	optimum	45
67770	positive	growth	37

culture pH

@ref	ability	type	pH	PH range
30446	positive	growth	5.5-9.5	alkaliphile
30446	positive	optimum	8.3

Physiology and metabolism

oxygen tolerance

@ref	oxygen tolerance	confidence
30446	anaerobe
125439	anaerobe	99.6

spore formation

@ref: 30446
spore formation: no

halophily

@ref	salt	growth	tested relation	concentration
30446	NaCl	positive	growth	6-30 %
30446	NaCl	positive	optimum	15 %

metabolite utilization

@ref	Chebi-ID	metabolite	utilization activity	kind of utilization tested
30446	28260	galactose	+	carbon source
30446	17754	glycerol	+	carbon source
30446	15361	pyruvate	+	carbon source
30446	16634	raffinose	+	carbon source
30446	33942	ribose	+	carbon source
30446	18222	xylose	+	carbon source

Isolation, sampling and environmental information

isolation

@ref	sample type	geographic location	country	origin.country	continent
16760	black sediment below the salt crust of a hypersaline lake	El Jerid lake	Tunisia	TUN	Africa
67770	Sediment collected below the salt crust on the hypersaline EI Jerid lake in southern Tunisia

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Lake (large)
#Environmental	#Terrestrial	#Sediment
#Condition	#Saline

Safety information

risk assessment

@ref: 16760
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 16760
description: Halanaerobacter jeridensis strain CEJFG43 16S ribosomal RNA gene, partial sequence
accession: GU265900
length: 1435
database: nuccore
NCBI tax ID: 706427

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Halanaerobacter jeridensis DSM 23230	GCA_016908315	contig	ncbi	706427
66792	Halanaerobacter jeridensis strain DSM 23230	706427.3	wgs	patric	706427
66792	Halanaerobacter jeridensis DSM 23230	2901107112	draft	img	706427

GC content

@ref	GC-content	method
16760	33.3	high performance liquid chromatography (HPLC)
30446	33.3

Genome-based predictions

predictions

@ref	model	trait	description	prediction	confidence	training_data
125438	gram-positive	gram-positive	Positive reaction to Gram-staining	no	81.245	no
125438	anaerobic	anaerobic	Ability to grow under anoxygenic conditions (including facultative anaerobes)	yes	87.172	yes
125438	spore-forming	spore-forming	Ability to form endo- or exospores	yes	57.268	no
125438	aerobic	aerobic	Ability to grow under oxygenic conditions (including facultative aerobes)	no	93.881	no
125438	thermophile	thermophilic	Ability to grow at temperatures above or equal to 45°C	no	88.066	yes
125438	motile2+	flagellated	Ability to perform flagellated movement	yes	76.432	no
125439	BacteriaNet	spore_formation	Ability to form endo- or exospores	yes	75.4
125439	BacteriaNet	motility	Ability to perform movement	yes	79.3
125439	BacteriaNet	gram_stain	Reaction to gram-staining	positive	84.4
125439	BacteriaNet	oxygen_tolerance	Oxygenic conditions needed for growth	anaerobe	99.6

External links

@ref: 16760

culture collection no.: DSM 23230, JCM 16696

straininfo link

@ref: 75471
straininfo: 402114

literature

topic: Phylogeny
Pubmed-ID: 22003033
title: Halanaerobacter jeridensis sp. nov., isolated from a hypersaline lake.
authors: Mezghani M, Alazard D, Karray F, Cayol JL, Joseph M, Postec A, Fardeau ML, Tholozan JL, Sayadi S
journal: Int J Syst Evol Microbiol
DOI: 10.1099/ijs.0.036301-0
year: 2011
mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Fermentation, Geologic Sediments/*microbiology, Gram-Negative Anaerobic Bacteria/*classification/genetics/isolation & purification, Lakes/*microbiology, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride, Tunisia, Water Microbiology
topic2: Genetics

Reference

@id	authors	catalogue	doi/url	title	ID_cross_reference	pubmed
16760	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23230)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-23230
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30446	Barberan A, Caceres Velazquez H, Jones S, Fierer N.		10.1128/mSphere.00237-17	Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information	26781	28776041
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770	Curators of the JCM		https://jcm.brc.riken.jp/en/
75471	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID402114.1	StrainInfo: A central database for resolving microbial strain identifiers
125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann		10.1101/2024.08.12.607695	Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets
125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy		https://github.com/GenomeNet/deepG	deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1