Strain identifier

BacDive ID: 5966

Type strain: Yes

Species: Natronococcus amylolyticus

Strain Designation: Ah-36

Strain history: CIP <- 1997, JCM <- T. Kudo: strain Ah-36

NCBI tax ID(s): 1227497 (strain), 44470 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 9.2 (current version):
version 9:
version 8.1:
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 9.2 (current version)

General

@ref: 3964

BacDive-ID: 5966

DSM-Number: 10524

keywords: genome sequence, 16S sequence, Archaea, obligate aerobe, mesophilic, Gram-positive, coccus-shaped

description: Natronococcus amylolyticus Ah-36 is an obligate aerobe, mesophilic, Gram-positive archaeon that was isolated from soda lake.

NCBI tax id

NCBI tax idMatching level
44470species
1227497strain

strain history

@refhistory
3964<- JCM <- T. Kobayashi, Nagoya University, strain Ah-36
67770T. Kudo Ah-36.
116358CIP <- 1997, JCM <- T. Kudo: strain Ah-36

doi: 10.13145/bacdive5966.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Halobacteria
  • order: Halobacteriales
  • family: Natrialbaceae
  • genus: Natronococcus
  • species: Natronococcus amylolyticus
  • full scientific name: Natronococcus amylolyticus Kanai et al. 1995

@ref: 3964

domain: Archaea

phylum: Euryarchaeota

class: Halobacteria

order: Halobacteriales

family: Natrialbaceae

genus: Natronococcus

species: Natronococcus amylolyticus

full scientific name: Natronococcus amylolyticus Kanai et al. 1995

strain designation: Ah-36

type strain: yes

Morphology

cell morphology

  • @ref: 116358
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3964NATRONOBACTERIA MEDIUM (DSMZ Medium 371)yeshttps://mediadive.dsmz.de/medium/371Name: NATRONOBACTERIA MEDIUM (DSMZ Medium 371) Composition: NaCl 200.0 g/l Agar 20.0 g/l Casamino acids 5.0 g/l Yeast extract 5.0 g/l Na2CO3 5.0 g/l Na2-glutamate 1.0 g/l KH2PO4 1.0 g/l KCl 1.0 g/l NH4Cl 1.0 g/l MgSO4 x 7 H2O 0.24 g/l CaSO4 x 2 H2O 0.17 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water
39267MEDIUM 225 - for Natronococcus, Amylolyticus and Natronobacterium gregoryiyesDistilled water make up to (1000.000 ml);Sodium chloride (200.000 g);Potassium chloride (1.000 g);Magnesium sulphate heptahydrate (0.240 g);Agar (20.000 g);Yeastextract (5.000 g);Potassium di-hydrogen phosphate (1.000 g);Ammonium chloride (1.000 g);Sodium
116358CIP Medium 225yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=225

culture temp

@refgrowthtypetemperature
3964positivegrowth37
39267positivegrowth37
67770positivegrowth37
116358positivegrowth25-37
116358nogrowth5
116358nogrowth15

Physiology and metabolism

oxygen tolerance

  • @ref: 116358
  • oxygen tolerance: obligate aerobe

compound production

@refcompound
3964alpha amylase
67770Maltotriose-forming alpha-amylase

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1163584853esculin-hydrolysis
116358606565hippurate-hydrolysis
11635817632nitrate-builds gas from
11635817632nitrate+reduction
11635816301nitrite-builds gas from
11635816301nitrite-reduction
11635817632nitrate-respiration

antibiotic resistance

  • @ref: 116358
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 116358
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11635815688acetoin-
11635817234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382leucine arylamidase-3.4.11.1
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
116358oxidase+
116358beta-galactosidase-3.2.1.23
116358gelatinase-
116358catalase+1.11.1.6
116358gamma-glutamyltransferase+2.3.2.2
116358phenylalanine ammonia-lyase-4.3.1.24
116358tryptophan deaminase-
116358urease-3.5.1.5
68382beta-glucuronidase-3.2.1.31
68382valine arylamidase-
68382lipase (C 14)-
68382esterase lipase (C 8)+

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116358--++-------+--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
3964soda lakeLake MagadiKenyaKENAfrica
67770Soil from shores of the Lake MagadiKenyaKENAfrica
116358Environment, Soil from sharesMagadi lakeKenyaKENAfrica

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Condition#Alkaline

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
39641Risk group (German classification)
1163581Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Natronococcus amylolyticus gene for 16S rRNA and gene for alanine tRNA, complete sequenceD436281930nuccore44470
20218Natronococcus amylolyticus gene for 16S rRNA, complete sequence, strain: JCM 9655AB6634681474nuccore44470

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Natronococcus amylolyticus DSM 105242554235477draftimg1227497
67770Natronococcus amylolyticus DSM 10524GCA_000337675contigncbi1227497
66792Natronococcus amylolyticus DSM 105241227497.3wgspatric1227497

GC content

  • @ref: 67770
  • GC-content: 63.5

External links

@ref: 3964

culture collection no.: DSM 10524, JCM 9655, ATCC 51971, CGMCC 1.3840, CIP 105333, FERM P-11024

straininfo link

  • @ref: 75455
  • straininfo: 45332

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny7547296Natronococcus amylolyticus sp. nov., a haloalkaliphilic archaeon.Kanal H, Kobayashi T, Aono R, Kudo TInt J Syst Bacteriol10.1099/00207713-45-4-7621995Archaea/*classification/genetics, Base Sequence, DNA, Bacterial/chemistry, DNA, Ribosomal/chemistry, Molecular Sequence Data, RNA, Ribosomal, 16S/geneticsGenetics
Phylogeny22345136Natronococcus roseus sp. nov., a haloalkaliphilic archaeon from a hypersaline lake.Corral P, Gutierrez MC, Castillo AM, Dominguez M, Lopalco P, Corcelli A, Ventosa AInt J Syst Evol Microbiol10.1099/ijs.0.036558-02012Base Composition, DNA, Archaeal/genetics, Geologic Sediments/microbiology, Lakes/microbiology, Molecular Sequence Data, Mongolia, Natronococcus/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/metabolism, Salinity, *Water MicrobiologyGenetics
Phylogeny34982242Natronococcus pandeyae sp. nov., a Novel Haloarchaeon from Sambhar Salt Lake.Kajale S, Deshpande N, Lodha T, Shouche Y, Sharma ACurr Microbiol10.1007/s00284-021-02740-12022DNA, Archaeal/genetics, *Halobacteriaceae/genetics, India, Lakes, *Natronococcus, Nucleic Acid Hybridization, Phospholipids, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3964Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10524)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10524
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39267Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17341
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
75455Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID45332.1StrainInfo: A central database for resolving microbial strain identifiers
116358Curators of the CIPCollection of Institut Pasteur (CIP 105333)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105333