Strain identifier
BacDive ID: 5894
Type strain:
Species: Natrinema salaciae
Strain Designation: MDB20
Strain history: M. S. da Costa MDB20.
NCBI tax ID(s): 1186196 (species)
version 8.1 (current version)
General
@ref: 17827
BacDive-ID: 5894
DSM-Number: 25054
keywords: 16S sequence, Archaea, thermophilic
description: Natrinema salaciae MDB20 is a thermophilic archaeon that was isolated from anoxic brine.
NCBI tax id
- NCBI tax id: 1186196
- Matching level: species
strain history
@ref | history |
---|---|
17827 | <- M. da Costa, Univ. Coimbra, Portugal; MDB20 <- M. Taborda |
67770 | M. S. da Costa MDB20. |
doi: 10.13145/bacdive5894.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Halobacteria
- order: Natrialbales
- family: Natrialbaceae
- genus: Natrinema
- species: Natrinema salaciae
- full scientific name: Natrinema salaciae Albuquerque et al. 2012
@ref: 17827
domain: Archaea
phylum: Euryarchaeota
class: Halobacteria
order: Halobacteriales
family: Natrialbaceae
genus: Natrinema
species: Natrinema salaciae
full scientific name: Natrinema salaciae Albuquerque et al. 2012
strain designation: MDB20
type strain: no
Morphology
colony morphology
- @ref: 17827
- incubation period: 3-7 days
Culture and growth conditions
culture medium
- @ref: 17827
- name: HALOBACTERIA MEDIUM (DSMZ Medium 372)
- growth: yes
- link: https://mediadive.dsmz.de/medium/372
- composition: Name: HALOBACTERIA MEDIUM (DSMZ Medium 372) Composition: NaCl 200.0 g/l MgSO4 x 7 H2O 20.0 g/l Agar 20.0 g/l Yeast extract 5.0 g/l Casamino acids 5.0 g/l Na3-citrate 3.0 g/l KCl 2.0 g/l Na glutamate 1.0 g/l FeCl2 x 4 H2O 0.036 g/l MnCl2 x 4 H2O 0.00036 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
17827 | positive | growth | 45 | thermophilic |
67770 | positive | growth | 45 | thermophilic |
Physiology and metabolism
observation
- @ref: 67770
- observation: quinones: MK-8, MK-8(H2)
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | latitude | longitude |
---|---|---|---|---|---|---|---|
17827 | anoxic brine | Eastern Mediterranean Sea, Lake Medee (34°19.778'N and 22°31.341'E) at a depth of 3050 m | Italy | ITA | Europe | 34.3296 | 22.5224 |
67770 | Brine from Lake Medee, at a depth of 3050 m |
isolation source categories
Cat1 | Cat2 |
---|---|
#Environmental | #Aquatic |
#Condition | #Anoxic (anaerobic) |
#Condition | #Saline |
taxonmaps
- @ref: 69479
- File name: preview.99_191737.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17652;96_69524;97_91960;98_124971;99_191737&stattab=map
- Last taxonomy: Natrinema salaciae subclade
- 16S sequence: JQ669384
- Sequence Identity:
- Total samples: 1
- soil counts: 1
Safety information
risk assessment
- @ref: 17827
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 17827
- description: Natrinema salaciae strain MDB20 16S ribosomal RNA gene, partial sequence
- accession: JQ669384
- length: 1454
- database: ena
- NCBI tax ID: 1186196
GC content
- @ref: 67770
- GC-content: 64.6
- method: high performance liquid chromatography (HPLC)
External links
@ref: 17827
culture collection no.: DSM 25054, JCM 17868
straininfo link
- @ref: 75374
- straininfo: 401057
literature
- topic: Phylogeny
- Pubmed-ID: 22817877
- title: Natrinema salaciae sp. nov., a halophilic archaeon isolated from the deep, hypersaline anoxic Lake Medee in the Eastern Mediterranean Sea.
- authors: Albuquerque L, Taborda M, La Cono V, Yakimov M, da Costa MS
- journal: Syst Appl Microbiol
- DOI: 10.1016/j.syapm.2012.06.005
- year: 2012
- mesh: Anaerobiosis, Genes, Archaeal/genetics, Halobacteriaceae/*classification/genetics/*isolation & purification, Italy, Lakes/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Salinity, Salts, Water Microbiology
- topic2: Genetics
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
17827 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25054) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-25054 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
75374 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID401057.1 | StrainInfo: A central database for resolving microbial strain identifiers |