Strain identifier

BacDive ID: 5894

Type strain: No

Species: Natrinema salaciae

Strain Designation: MDB20

Strain history: M. S. da Costa MDB20.

NCBI tax ID(s): 1186196 (species)

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General

@ref: 17827

BacDive-ID: 5894

DSM-Number: 25054

keywords: 16S sequence, Archaea, thermophilic

description: Natrinema salaciae MDB20 is a thermophilic archaeon that was isolated from anoxic brine.

NCBI tax id

  • NCBI tax id: 1186196
  • Matching level: species

strain history

@refhistory
17827<- M. da Costa, Univ. Coimbra, Portugal; MDB20 <- M. Taborda
67770M. S. da Costa MDB20.

doi: 10.13145/bacdive5894.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Halobacteria
  • order: Natrialbales
  • family: Natrialbaceae
  • genus: Natrinema
  • species: Natrinema salaciae
  • full scientific name: Natrinema salaciae Albuquerque et al. 2012

@ref: 17827

domain: Archaea

phylum: Euryarchaeota

class: Halobacteria

order: Halobacteriales

family: Natrialbaceae

genus: Natrinema

species: Natrinema salaciae

full scientific name: Natrinema salaciae Albuquerque et al. 2012

strain designation: MDB20

type strain: no

Morphology

colony morphology

  • @ref: 17827
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

  • @ref: 17827
  • name: HALOBACTERIA MEDIUM (DSMZ Medium 372)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/372
  • composition: Name: HALOBACTERIA MEDIUM (DSMZ Medium 372) Composition: NaCl 200.0 g/l MgSO4 x 7 H2O 20.0 g/l Agar 20.0 g/l Yeast extract 5.0 g/l Casamino acids 5.0 g/l Na3-citrate 3.0 g/l KCl 2.0 g/l Na glutamate 1.0 g/l FeCl2 x 4 H2O 0.036 g/l MnCl2 x 4 H2O 0.00036 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
17827positivegrowth45thermophilic
67770positivegrowth45thermophilic

Physiology and metabolism

observation

  • @ref: 67770
  • observation: quinones: MK-8, MK-8(H2)

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
17827anoxic brineEastern Mediterranean Sea, Lake Medee (34°19.778'N and 22°31.341'E) at a depth of 3050 mItalyITAEurope34.329622.5224
67770Brine from Lake Medee, at a depth of 3050 m

isolation source categories

Cat1Cat2
#Environmental#Aquatic
#Condition#Anoxic (anaerobic)
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_191737.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17652;96_69524;97_91960;98_124971;99_191737&stattab=map
  • Last taxonomy: Natrinema salaciae subclade
  • 16S sequence: JQ669384
  • Sequence Identity:
  • Total samples: 1
  • soil counts: 1

Safety information

risk assessment

  • @ref: 17827
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 17827
  • description: Natrinema salaciae strain MDB20 16S ribosomal RNA gene, partial sequence
  • accession: JQ669384
  • length: 1454
  • database: ena
  • NCBI tax ID: 1186196

GC content

  • @ref: 67770
  • GC-content: 64.6
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 17827

culture collection no.: DSM 25054, JCM 17868

straininfo link

  • @ref: 75374
  • straininfo: 401057

literature

  • topic: Phylogeny
  • Pubmed-ID: 22817877
  • title: Natrinema salaciae sp. nov., a halophilic archaeon isolated from the deep, hypersaline anoxic Lake Medee in the Eastern Mediterranean Sea.
  • authors: Albuquerque L, Taborda M, La Cono V, Yakimov M, da Costa MS
  • journal: Syst Appl Microbiol
  • DOI: 10.1016/j.syapm.2012.06.005
  • year: 2012
  • mesh: Anaerobiosis, Genes, Archaeal/genetics, Halobacteriaceae/*classification/genetics/*isolation & purification, Italy, Lakes/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Salinity, Salts, Water Microbiology
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
17827Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25054)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25054
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75374Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID401057.1StrainInfo: A central database for resolving microbial strain identifiers