Strain identifier

BacDive ID: 5776

Type strain: Yes

Species: Fusobacterium necrophorum subsp. funduliforme

Strain Designation: Fn524, Fn 524, 6161

Strain history: CIP <- 1996, JCM <- T. shinjo: strain Fn 524 <- M. Kanoe <- VPI: strain 6161

NCBI tax ID(s): 1095747 (strain), 143387 (subspecies)

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General

@ref: 8249

BacDive-ID: 5776

DSM-Number: 19678

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, human pathogen

description: Fusobacterium necrophorum subsp. funduliforme Fn524 is an anaerobe, mesophilic human pathogen that was isolated from bovine liver abscess.

NCBI tax id

NCBI tax idMatching level
143387subspecies
1095747strain

strain history

@refhistory
8249<- JCM <- T. Shinjo; Fn524 <- M. Kanoe <- VPI; strain 6161
67770T. Shinjo Fn 524 (Biovar B) <-- M. Kanoe <-- VPI 6161.
121253CIP <- 1996, JCM <- T. shinjo: strain Fn 524 <- M. Kanoe <- VPI: strain 6161

doi: 10.13145/bacdive5776.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/fusobacteriota
  • domain: Bacteria
  • phylum: Fusobacteriota
  • class: Fusobacteriia
  • order: Fusobacteriales
  • family: Fusobacteriaceae
  • genus: Fusobacterium
  • species: Fusobacterium necrophorum subsp. funduliforme
  • full scientific name: Fusobacterium necrophorum subsp. funduliforme (ex Hallé 1898) Shinjo et al. 1991

@ref: 8249

domain: Bacteria

phylum: Fusobacteria

class: Fusobacteriia

order: Fusobacteriales

family: Fusobacteriaceae

genus: Fusobacterium

species: Fusobacterium necrophorum subsp. funduliforme

full scientific name: Fusobacterium necrophorum subsp. funduliforme (ex Hallé 1898) Shinjo et al. 1991

strain designation: Fn524, Fn 524, 6161

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8249COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8249CHOPPED MEAT MEDIUM (DSMZ Medium 78)yeshttps://mediadive.dsmz.de/medium/78Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water
41348MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
121253CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperaturerange
8249positivegrowth37mesophilic
41348positivegrowth37mesophilic
55350positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8249anaerobe
55350anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan+energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase+4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    55350C12:03.112
    55350C13:00.513
    55350C14:011.914
    55350C15:00.715
    55350C16:012.316
    55350C18:02.318
    55350C13:0 3OH/C15:1 i I/H2.914.469
    55350C13:0 ISO 2OH0.913.814
    55350C13:1 at 12-130.612.931
    55350C14:0 3OH/C16:1 ISO I9.715.485
    55350C14:1 ω5c0.113.899
    55350C15:0 ANTEISO1.814.711
    55350C15:0 ISO 3OH1.616.135
    55350C15:1 ω8c0.714.792
    55350C16:0 3OH0.917.52
    55350C16:1 ω5c0.515.908
    55350C16:1 ω7c9.615.819
    55350C16:1 ω9c1.915.774
    55350C17:1 ISO I/C16:0 DMA5.616.481
    55350C17:1 ω8c0.416.792
    55350C18:1 ω7c /12t/9t0.917.824
    55350C18:1 ω9c5.917.769
    55350C18:2 ω6,9c/C18:0 ANTE8.317.724
    55350Unidentified3.59.152
    55350Unidentified0.39.438
    55350Unidentified0.412.262
    55350Unidentified0.513.766
    55350Unidentified2.114.28
    55350Unidentified114.948
    55350Unidentified4.916.296
    55350Unidentified118.057
    55350Unidentified1.518.098
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
8249---------------+-------------
8249---------------+-------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
8249bovine liver abscessJapanJPNAsia
55350Bovine liver abscess
67770Bovine liver abscess
121253Animal, Bovine liver abscess

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body-Site#Other#Abscess
#Host Body-Site#Organ#Liver

taxonmaps

  • @ref: 69479
  • File name: preview.99_1526.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_140;96_874;97_1022;98_1205;99_1526&stattab=map
  • Last taxonomy: Fusobacterium necrophorum
  • 16S sequence: AM905356
  • Sequence Identity:
  • Total samples: 30069
  • soil counts: 1769
  • aquatic counts: 1051
  • animal counts: 26978
  • plant counts: 271

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
8249yesyes2Risk group (German classification)
1212532Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Fusobacterium necrophorum subsp. funduliforme gene for 16S ribosomal RNA, paertial sequence, strain: JCM 3724AB5254131469ena143387
20218Fusobacterium necrophorum subsp. funduliforme strain JCM 3724 clone 1 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer and tRNA-Ile gene, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF369878537ena143387
20218Fusobacterium necrophorum subsp. funduliforme strain JCM 3724 clone 2 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF369879362ena143387
8249Fusobacterium necrophorum subsp. funduliforme partial 16S rRNA gene, strain DSM 19678TAM9053561487ena143387
8249Fusobacterium necrophorum subsp. funduliforme strain ATCC 51357 16S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF342845746ena143387

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Fusobacterium necrophorum subsp. funduliforme ATCC 513571095747.3wgspatric1095747
66792Fusobacterium necrophorum subsp. funduliforme strain CCUG 42162143387.88wgspatric143387
66792Fusobacterium necrophorum funduliforme ATCC 513572537561963draftimg1095747
67770Fusobacterium necrophorum subsp. funduliforme ATCC 51357GCA_000262225contigncbi1095747
67770Fusobacterium necrophorum subsp. funduliforme CCUG 42162GCA_008801775contigncbi143387

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno91.939no
flagellatedno94.732no
gram-positiveno92.347no
anaerobicyes99.999no
aerobicno98.579no
halophileno75.091no
spore-formingno91.212no
glucose-utilyes81.225no
thermophileno97.272no
glucose-fermentyes51.6no

External links

@ref: 8249

culture collection no.: DSM 19678, ATCC 51357, CCUG 42162, CIP 104859, JCM 3724, VPI 6161

straininfo link

  • @ref: 75255
  • straininfo: 46149

literature

  • topic: Phylogeny
  • Pubmed-ID: 1883714
  • title: Proposal of two subspecies of Fusobacterium necrophorum (Flugge) Moore and Holdeman: Fusobacterium necrophorum subsp. necrophorum subsp. nov., nom. rev. (ex Flugge 1886), and Fusobacterium necrophorum subsp. funduliforme subsp. nov., nom. rev. (ex Halle 1898).
  • authors: Shinjo T, Fujisawa T, Mitsuoka T
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-41-3-395
  • year: 1991
  • mesh: Base Composition, DNA, Bacterial, Fusobacterium necrophorum/*classification/genetics/metabolism, Sequence Homology, Nucleic Acid
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8249Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19678)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19678
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41348Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16814
55350Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 42162)https://www.ccug.se/strain?id=42162
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75255Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46149.1StrainInfo: A central database for resolving microbial strain identifiers
121253Curators of the CIPCollection of Institut Pasteur (CIP 104859)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104859