Strain identifier

BacDive ID: 5770

Type strain: Yes

Species: Fusobacterium mortiferum

Strain Designation: 350A

Strain history: <- CCUG; CCUG 14475 <- ATCC; ATCC 25557 <- W. E. C. Moore; VPI 4123A <- A. Prevot; 350A

NCBI tax ID(s): 850 (species)

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General

@ref: 8328

BacDive-ID: 5770

DSM-Number: 19809

keywords: 16S sequence, Bacteria, anaerobe

description: Fusobacterium mortiferum 350A is an anaerobe bacterium that was isolated from human maxillary abscess.

NCBI tax id

  • NCBI tax id: 850
  • Matching level: species

strain history

  • @ref: 8328
  • history: <- CCUG; CCUG 14475 <- ATCC; ATCC 25557 <- W. E. C. Moore; VPI 4123A <- A. Prevot; 350A

doi: 10.13145/bacdive5770.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/fusobacteriota
  • domain: Bacteria
  • phylum: Fusobacteriota
  • class: Fusobacteriia
  • order: Fusobacteriales
  • family: Fusobacteriaceae
  • genus: Fusobacterium
  • species: Fusobacterium mortiferum
  • full scientific name: Fusobacterium mortiferum (Harris 1901) Moore and Holdeman 1970 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Bacillus mortiferus
    20215Hiblerillus rectus
    20215Clostridium rectum

@ref: 8328

domain: Bacteria

phylum: Fusobacteria

class: Fusobacteriia

order: Fusobacteriales

family: Fusobacteriaceae

genus: Fusobacterium

species: Fusobacterium mortiferum

full scientific name: Fusobacterium mortiferum (Harris 1901) Moore and Holdeman 1970 emend. De Witte et al. 2017

strain designation: 350A

type strain: yes

Morphology

colony morphology

  • @ref: 46109
  • incubation period: 2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8328PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
8328COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperature
8328positivegrowth37
46109positivegrowth37

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8328anaerobe
46109anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose+fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase+3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate+
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
8328--+++-----++/-----+-----+------
8328--+++-----++----+-----+------

Isolation, sampling and environmental information

isolation

@refsample type
8328human maxillary abscess
46109Human maxillary abscess

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Oral cavity and airways
#Host Body-Site#Other#Abscess

taxonmaps

  • @ref: 69479
  • File name: preview.99_7123.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_140;96_3341;97_4098;98_5240;99_7123&stattab=map
  • Last taxonomy: Fusobacterium mortiferum
  • 16S sequence: HG324078
  • Sequence Identity:
  • Total samples: 32499
  • soil counts: 447
  • aquatic counts: 2288
  • animal counts: 29675
  • plant counts: 89

Safety information

risk assessment

  • @ref: 8328
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Fusobacterium mortiferum 16S rRNA gene, strain ATCC 25557AJ8670321495nuccore850
20218Fusobacterium mortiferum partial 16S rRNA gene, type strain DSM 19809THG3240781482nuccore850
20218Fusobacterium mortiferum rRNA small subunitX554141333nuccore850
124043Fusobacterium mortiferum partial 16S rRNA gene, strain CCUG 14475LT5746751367nuccore850

External links

@ref: 8328

culture collection no.: DSM 19809, ATCC 25557, CCUG 14475, VPI 4123A

straininfo link

  • @ref: 75249
  • straininfo: 38596

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism1533618Sucrose fermentation by Fusobacterium mortiferum ATCC 25557: transport, catabolism, and products.Thompson J, Nguyen NY, Robrish SAJ Bacteriol10.1128/jb.174.10.3227-3235.19921992Acetates/metabolism, Acetic Acid, Amino Acid Sequence, Biological Transport, Active, Butyrates/metabolism, Enzyme Induction, Fusobacterium/*metabolism, Glycoside Hydrolases/isolation & purification/metabolism, Lactates/metabolism, Lactic Acid, Molecular Sequence Data, Phosphoenolpyruvate Sugar Phosphotransferase System/metabolism, Phosphofructokinase-1/isolation & purification/metabolism, Substrate Specificity, Sucrose/*metabolism, beta-FructofuranosidaseEnzymology
Metabolism1937813Sugar metabolism by fusobacteria: regulation of transport, phosphorylation, and polymer formation by Fusobacterium mortiferum ATCC 25557.Robrish SA, Oliver C, Thompson JInfect Immun10.1128/iai.59.12.4547-4554.19911991Biological Transport, *Carbohydrate Metabolism, Fusobacterium/growth & development/*metabolism, Glucose/metabolism, Glycogen/metabolism, Phosphorylation, Polysaccharides/*metabolism
Pathogenicity2640313Effects of periodontopathic bacteria on IL-1 and IL-1 inhibitor production by human polymorphonuclear neutrophils.Yamazaki K, Polak B, Bird PS, Gemmell E, Hara K, Seymour GJOral Microbiol Immunol10.1111/j.1399-302x.1989.tb00251.x1989*Actinomyces, *Bacteroides, *Fusobacterium, Growth Inhibitors/biosynthesis, Humans, Interleukin-1/antagonists & inhibitors/*biosynthesis, Lymphokines/*biosynthesis, Neutrophils/*metabolism, Periodontal Diseases/*microbiologyBiotechnology
Enzymology7730284Purification from Fusobacterium mortiferum ATCC 25557 of a 6-phosphoryl-O-alpha-D-glucopyranosyl:6-phosphoglucohydrolase that hydrolyzes maltose 6-phosphate and related phospho-alpha-D-glucosides.Thompson J, Gentry-Weeks CR, Nguyen NY, Folk JE, Robrish SAJ Bacteriol10.1128/jb.177.9.2505-2512.19951995Amino Acid Sequence, Carbohydrate Sequence, Enzyme Stability, Fusobacterium/*enzymology, Glucosephosphates/*metabolism, Glucosides/*metabolism, Isoelectric Focusing, Models, Biological, Molecular Sequence Data, Sequence Analysis, Sequence Homology, Amino Acid, Substrate Specificity, Sugar Phosphates/*metabolism, alpha-Glucosidases/biosynthesis/isolation & purification/*metabolismMetabolism
Metabolism8195080Phosphoenolpyruvate-dependent maltose:phosphotransferase activity in Fusobacterium mortiferum ATCC 25557: specificity, inducibility, and product analysis.Robrish SA, Fales HM, Gentry-Weeks C, Thompson JJ Bacteriol10.1128/jb.176.11.3250-3256.19941994Aerobiosis, Anaerobiosis, Carbohydrate Sequence, Cell Membrane Permeability, Enzyme Induction, Fusobacterium/*enzymology, Glucosides/metabolism, Hydrolysis, Maltose/*metabolism, Molecular Sequence Data, Phosphoenolpyruvate Sugar Phosphotransferase System/*metabolism, Phosphorylation, Stereoisomerism, Sugar Phosphates/*isolation & purification/metabolismEnzymology
Pathogenicity9573823Helicobacter pylori adheres selectively to Fusobacterium spp.Andersen RN, Ganeshkumar N, Kolenbrander PEOral Microbiol Immunol10.1111/j.1399-302x.1998.tb00751.x1998*Bacterial Adhesion/drug effects, Culture Media, Depression, Chemical, Fusobacterium/drug effects/*physiology, Helicobacter pylori/drug effects/*physiology, Hot Temperature, Humans, Lactose/pharmacology, Mouth/microbiology, Species SpecificityCultivation
Phylogeny27312552Clinical Fusobacterium mortiferum Isolates Cluster with Undifferentiated Clostridium rectum Species Based on 16S rRNA Gene Phylogenetic Analysis.Lee Y, Eun CS, Han DSAnn Clin Lab Sci46/3/2792016Base Sequence, Clostridium/*genetics, Fusobacterium/*genetics/*isolation & purification, Humans, *Phylogeny, RNA, Ribosomal, 16S/*geneticsPathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8328Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19809)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19809
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
46109Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 14475)https://www.ccug.se/strain?id=14475
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75249Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID38596.1StrainInfo: A central database for resolving microbial strain identifiers
124043Dr. Isabel Schober, Dr. Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy