Strain identifier

BacDive ID: 5628

Type strain: Yes

Species: Psychroflexus gondwanensis

Strain history: CIP <- 1994, ACAM

NCBI tax ID(s): 1189619 (strain), 251 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 2160

BacDive-ID: 5628

DSM-Number: 5423

keywords: 16S sequence, Bacteria, mesophilic, Gram-negative, rod-shaped

description: Psychroflexus gondwanensis DSM 5423 is a mesophilic, Gram-negative, rod-shaped bacterium that was isolated from organic lake.

NCBI tax id

NCBI tax idMatching level
251species
1189619strain

strain history

@refhistory
2160<- P. Franzmann <- S.J. Dobson <- R. Garric
409401994, ACAM
119704CIP <- 1994, ACAM

doi: 10.13145/bacdive5628.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Psychroflexus
  • species: Psychroflexus gondwanensis
  • full scientific name: Psychroflexus gondwanensis corrig. (Dobson et al. 1993) Bowman et al. 1999
  • synonyms

    @refsynonym
    20215Flavobacterium gondwanense
    20215Psychroflexus gondwanense

@ref: 2160

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Psychroflexus

species: Psychroflexus gondwanensis

full scientific name: Psychroflexus gondwanensis (Dobson et al. 1993) Bowman et al. 1999 emend. Hahnke et al. 2016

type strain: yes

Morphology

cell morphology

  • @ref: 119704
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2160BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
40940Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
119704CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
2160positivegrowth28mesophilic
40940positivegrowth22psychrophilic
119704positivegrowth22-25psychrophilic
119704nogrowth5psychrophilic
119704nogrowth15psychrophilic
119704nogrowth37mesophilic
119704nogrowth41thermophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11970416947citrate-carbon source
1197044853esculin+hydrolysis
11970417632nitrate-reduction
11970416301nitrite-reduction

enzymes

@refvalueactivityec
119704oxidase+
119704gelatinase+
119704amylase-
119704DNase+
119704caseinase-3.4.21.50
119704catalase+1.11.1.6
119704gamma-glutamyltransferase+2.3.2.2
119704lecithinase-
119704lipase-

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    51523C15:01.215
    51523C12:0 3OH3.413.455
    51523C13:0 3OH/C15:1 i I/H0.814.469
    51523C13:0 ANTEISO0.512.701
    51523C14:0 ISO6.613.618
    51523C14:0 ISO 3OH0.815.117
    51523C15:0 2OH416.217
    51523C15:0 3OH0.316.504
    51523C15:0 ANTEISO32.814.711
    51523C15:0 ISO1.514.621
    51523C15:1 ANTEISO A16.714.526
    51523C16:0 iso5.915.626
    51523C16:0 iso 3OH17.617.145
    51523C17:0 iso 3OH0.818.161
    51523Unidentified1.115.59
    51523Unidentified5.918.272
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

Isolation, sampling and environmental information

isolation

@refsample typecontinentsampling dategeographic locationcountryorigin.countryisolation date
2160organic lakeAustralia and Oceania
51523Australia and Oceania1987Vestfold HillsAntarcticaATA
119704Environment, Organic lake, depth 5mAntarcticaAntarcticaATA1987

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Lake (large)

taxonmaps

  • @ref: 69479
  • File name: preview.99_5257.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_423;96_1937;97_2714;98_3370;99_5257&stattab=map
  • Last taxonomy: Psychroflexus gondwanensis
  • 16S sequence: JX986967
  • Sequence Identity:
  • Total samples: 203
  • soil counts: 12
  • aquatic counts: 184
  • animal counts: 7

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
21601Risk group (German classification)
1197041Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Psychroflexus gondwanensis partial 16S rRNA gene, type strain DSM 5423TFR8499111476ena1189619
20218Flavobacterium gondwanense 16S ribosomal RNA geneM922781476ena251
2160Psychroflexus gondwanensis strain LMG 13192 16S ribosomal RNA gene, partial sequenceJX9869671475ena251

GC content

  • @ref: 2160
  • GC-content: 35.8
  • method: sequence analysis

External links

@ref: 2160

culture collection no.: DSM 5423, ACAM 44, ATCC 51278, LMG 13192, CCUG 33444, CIP 104040

straininfo link

  • @ref: 75111
  • straininfo: 7703

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny7678983Direct sequencing of the polymerase chain reaction-amplified 16S rRNA gene of Flavobacterium gondwanense sp. nov. and Flavobacterium salegens sp. nov., two new species from a hypersaline Antarctic lake.Dobson SJ, Colwell RR, McMeekin TA, Franzmann PDInt J Syst Bacteriol10.1099/00207713-43-1-771993Antarctic Regions, Bacterial Typing Techniques, Base Sequence, Flavobacterium/*classification/*genetics, *Genes, Bacterial, Molecular Sequence Data, Phylogeny, *Polymerase Chain Reaction/methods, RNA, Bacterial/*analysis, RNA, Ribosomal, 16S/*analysis/genetics, Sequence Analysis, Sodium Chloride, *Water MicrobiologyGenetics
Phylogeny21984670Psychroflexus halocasei sp. nov., isolated from a microbial consortium on a cheese.Seiler H, Bleicher A, Busse HJ, Hufner J, Scherer SInt J Syst Evol Microbiol10.1099/ijs.0.034801-02011Bacterial Typing Techniques, Base Composition, Cheese/*microbiology, DNA, Bacterial/genetics, Fatty Acids/analysis, Flavobacteriaceae/*classification/genetics/isolation & purification, *Food Microbiology, *Microbial Consortia, Molecular Sequence Data, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/analysisBiotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2160Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5423)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5423
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40940Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15904
51523Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 33444)https://www.ccug.se/strain?id=33444
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75111Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID7703.1StrainInfo: A central database for resolving microbial strain identifiers
119704Curators of the CIPCollection of Institut Pasteur (CIP 104040)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104040