Strain identifier
BacDive ID: 5628
Type strain:
Species: Psychroflexus gondwanensis
Strain history: CIP <- 1994, ACAM
NCBI tax ID(s): 1189619 (strain), 251 (species)
General
@ref: 2160
BacDive-ID: 5628
DSM-Number: 5423
keywords: 16S sequence, Bacteria, mesophilic, Gram-negative, rod-shaped
description: Psychroflexus gondwanensis DSM 5423 is a mesophilic, Gram-negative, rod-shaped bacterium that was isolated from organic lake.
NCBI tax id
NCBI tax id | Matching level |
---|---|
251 | species |
1189619 | strain |
strain history
@ref | history |
---|---|
2160 | <- P. Franzmann <- S.J. Dobson <- R. Garric |
40940 | 1994, ACAM |
119704 | CIP <- 1994, ACAM |
doi: 10.13145/bacdive5628.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacteroidota
- domain: Bacteria
- phylum: Bacteroidota
- class: Flavobacteriia
- order: Flavobacteriales
- family: Flavobacteriaceae
- genus: Psychroflexus
- species: Psychroflexus gondwanensis
- full scientific name: Psychroflexus gondwanensis corrig. (Dobson et al. 1993) Bowman et al. 1999
synonyms
@ref synonym 20215 Flavobacterium gondwanense 20215 Psychroflexus gondwanense
@ref: 2160
domain: Bacteria
phylum: Bacteroidetes
class: Flavobacteriia
order: Flavobacteriales
family: Flavobacteriaceae
genus: Psychroflexus
species: Psychroflexus gondwanensis
full scientific name: Psychroflexus gondwanensis (Dobson et al. 1993) Bowman et al. 1999 emend. Hahnke et al. 2016
type strain: yes
Morphology
cell morphology
- @ref: 119704
- gram stain: negative
- cell shape: rod-shaped
- motility: no
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
2160 | BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) | yes | https://mediadive.dsmz.de/medium/514 | Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water |
40940 | Marine agar (MA) | yes | Distilled water make up to (1000.000 ml);Marine agar (55.100 g) | |
119704 | CIP Medium 13 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
2160 | positive | growth | 28 | mesophilic |
40940 | positive | growth | 22 | psychrophilic |
119704 | positive | growth | 22-25 | psychrophilic |
119704 | no | growth | 5 | psychrophilic |
119704 | no | growth | 15 | psychrophilic |
119704 | no | growth | 37 | mesophilic |
119704 | no | growth | 41 | thermophilic |
Physiology and metabolism
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
119704 | 16947 | citrate | - | carbon source |
119704 | 4853 | esculin | + | hydrolysis |
119704 | 17632 | nitrate | - | reduction |
119704 | 16301 | nitrite | - | reduction |
enzymes
@ref | value | activity | ec |
---|---|---|---|
119704 | oxidase | + | |
119704 | gelatinase | + | |
119704 | amylase | - | |
119704 | DNase | + | |
119704 | caseinase | - | 3.4.21.50 |
119704 | catalase | + | 1.11.1.6 |
119704 | gamma-glutamyltransferase | + | 2.3.2.2 |
119704 | lecithinase | - | |
119704 | lipase | - |
fatty acid profile
fatty acids
@ref fatty acid percentage ECL 51523 C15:0 1.2 15 51523 C12:0 3OH 3.4 13.455 51523 C13:0 3OH/C15:1 i I/H 0.8 14.469 51523 C13:0 ANTEISO 0.5 12.701 51523 C14:0 ISO 6.6 13.618 51523 C14:0 ISO 3OH 0.8 15.117 51523 C15:0 2OH 4 16.217 51523 C15:0 3OH 0.3 16.504 51523 C15:0 ANTEISO 32.8 14.711 51523 C15:0 ISO 1.5 14.621 51523 C15:1 ANTEISO A 16.7 14.526 51523 C16:0 iso 5.9 15.626 51523 C16:0 iso 3OH 17.6 17.145 51523 C17:0 iso 3OH 0.8 18.161 51523 Unidentified 1.1 15.59 51523 Unidentified 5.9 18.272 - type of FA analysis: whole cell analysis
- method/protocol: CCUG
Isolation, sampling and environmental information
isolation
@ref | sample type | continent | sampling date | geographic location | country | origin.country | isolation date |
---|---|---|---|---|---|---|---|
2160 | organic lake | Australia and Oceania | |||||
51523 | Australia and Oceania | 1987 | Vestfold Hills | Antarctica | ATA | ||
119704 | Environment, Organic lake, depth 5m | Antarctica | Antarctica | ATA | 1987 |
isolation source categories
- Cat1: #Environmental
- Cat2: #Aquatic
- Cat3: #Lake (large)
taxonmaps
- @ref: 69479
- File name: preview.99_5257.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_423;96_1937;97_2714;98_3370;99_5257&stattab=map
- Last taxonomy: Psychroflexus gondwanensis
- 16S sequence: JX986967
- Sequence Identity:
- Total samples: 203
- soil counts: 12
- aquatic counts: 184
- animal counts: 7
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
2160 | 1 | Risk group (German classification) |
119704 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Psychroflexus gondwanensis partial 16S rRNA gene, type strain DSM 5423T | FR849911 | 1476 | ena | 1189619 |
20218 | Flavobacterium gondwanense 16S ribosomal RNA gene | M92278 | 1476 | ena | 251 |
2160 | Psychroflexus gondwanensis strain LMG 13192 16S ribosomal RNA gene, partial sequence | JX986967 | 1475 | ena | 251 |
GC content
- @ref: 2160
- GC-content: 35.8
- method: sequence analysis
External links
@ref: 2160
culture collection no.: DSM 5423, ACAM 44, ATCC 51278, LMG 13192, CCUG 33444, CIP 104040
straininfo link
- @ref: 75111
- straininfo: 7703
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 7678983 | Direct sequencing of the polymerase chain reaction-amplified 16S rRNA gene of Flavobacterium gondwanense sp. nov. and Flavobacterium salegens sp. nov., two new species from a hypersaline Antarctic lake. | Dobson SJ, Colwell RR, McMeekin TA, Franzmann PD | Int J Syst Bacteriol | 10.1099/00207713-43-1-77 | 1993 | Antarctic Regions, Bacterial Typing Techniques, Base Sequence, Flavobacterium/*classification/*genetics, *Genes, Bacterial, Molecular Sequence Data, Phylogeny, *Polymerase Chain Reaction/methods, RNA, Bacterial/*analysis, RNA, Ribosomal, 16S/*analysis/genetics, Sequence Analysis, Sodium Chloride, *Water Microbiology | Genetics |
Phylogeny | 21984670 | Psychroflexus halocasei sp. nov., isolated from a microbial consortium on a cheese. | Seiler H, Bleicher A, Busse HJ, Hufner J, Scherer S | Int J Syst Evol Microbiol | 10.1099/ijs.0.034801-0 | 2011 | Bacterial Typing Techniques, Base Composition, Cheese/*microbiology, DNA, Bacterial/genetics, Fatty Acids/analysis, Flavobacteriaceae/*classification/genetics/isolation & purification, *Food Microbiology, *Microbial Consortia, Molecular Sequence Data, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/analysis | Biotechnology |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
2160 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5423) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-5423 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
40940 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/15904 | ||||
51523 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 33444) | https://www.ccug.se/strain?id=33444 | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
75111 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID7703.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
119704 | Curators of the CIP | Collection of Institut Pasteur (CIP 104040) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104040 |