Strain identifier

BacDive ID: 554

Type strain: Yes

Species: Anoxybacillus contaminans

Strain Designation: R-16222

Strain history: <- D. Janssens, LMG <- E. De Clerck, Univ. Ghent, Belgium; R-16222

NCBI tax ID(s): 225988 (species)

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General

@ref: 6128

BacDive-ID: 554

DSM-Number: 15866

keywords: 16S sequence, Bacteria, facultative anaerobe, spore-forming, thermophilic, Gram-variable, motile, rod-shaped

description: Anoxybacillus contaminans R-16222 is a facultative anaerobe, spore-forming, thermophilic bacterium that was isolated from gelatin.

NCBI tax id

  • NCBI tax id: 225988
  • Matching level: species

strain history

  • @ref: 6128
  • history: <- D. Janssens, LMG <- E. De Clerck, Univ. Ghent, Belgium; R-16222

doi: 10.13145/bacdive554.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Anoxybacillus
  • species: Anoxybacillus contaminans
  • full scientific name: Anoxybacillus contaminans De Clerck et al. 2004

@ref: 6128

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Anoxybacillus

species: Anoxybacillus contaminans

full scientific name: Anoxybacillus contaminans De Clerck et al. 2004

strain designation: R-16222

type strain: yes

Morphology

cell morphology

  • @ref: 30118
  • gram stain: variable
  • cell length: 7 µm
  • cell width: 0.85 µm
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 30118
  • production: no

Culture and growth conditions

culture medium

  • @ref: 6128
  • name: CASO AGAR (MERCK 105458) (DSMZ Medium 220)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/220
  • composition: Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
6128positivegrowth50thermophilic
30118positiveoptimum50thermophilic

culture pH

@refabilitytypepHPH range
30118positivegrowth4.5-9.5alkaliphile
30118positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 30118
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 30118
  • spore formation: yes

halophily

  • @ref: 30118
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: <5 %

observation

  • @ref: 30118
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3011822599arabinose+carbon source
3011828757fructose+carbon source
3011828260galactose+carbon source
301185291gelatin+carbon source
3011817234glucose+carbon source
3011817754glycerol+carbon source
3011828087glycogen+carbon source
3011817306maltose+carbon source
3011837684mannose+carbon source
3011837657methyl D-glucoside+carbon source
30118506227N-acetylglucosamine+carbon source
3011816634raffinose+carbon source
3011833942ribose+carbon source
3011817992sucrose+carbon source
3011827082trehalose+carbon source
3011818222xylose+carbon source
301184853esculin+hydrolysis
3011817632nitrate+reduction

enzymes

@refvalueactivityec
30118catalase+1.11.1.6
30118gelatinase+

Isolation, sampling and environmental information

isolation

  • @ref: 6128
  • sample type: gelatin
  • country: France
  • origin.country: FRA
  • continent: Europe

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Industrial
  • Cat3: #Engineered product

taxonmaps

  • @ref: 69479
  • File name: preview.99_9471.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_79;96_465;97_532;98_6991;99_9471&stattab=map
  • Last taxonomy: Anoxybacillus contaminans subclade
  • 16S sequence: AJ551330
  • Sequence Identity:
  • Total samples: 497
  • soil counts: 196
  • aquatic counts: 98
  • animal counts: 184
  • plant counts: 19

Safety information

risk assessment

  • @ref: 6128
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 30118
  • description: Anoxybacillus contaminans partial 16S rRNA gene, type strain LMG 21881T
  • accession: AJ551330
  • length: 1541
  • database: nuccore
  • NCBI tax ID: 225988

GC content

  • @ref: 30118
  • GC-content: 44.4

External links

@ref: 6128

culture collection no.: DSM 15866, LMG 21881

straininfo link

  • @ref: 70232
  • straininfo: 89484

literature

  • topic: Phylogeny
  • Pubmed-ID: 15143046
  • title: Anoxybacillus contaminans sp. nov. and Bacillus gelatini sp. nov., isolated from contaminated gelatin batches.
  • authors: De Clerck E, Rodriguez-Diaz M, Vanhoutte T, Heyrman J, Logan NA, De Vos P
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.02960-0
  • year: 2004
  • mesh: Bacillaceae/*classification/genetics/*isolation & purification/metabolism, Bacillus/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, *Drug Contamination, *Gelatin, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
6128Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15866)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15866
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30118Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2647328776041
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
70232Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID89484.1StrainInfo: A central database for resolving microbial strain identifiers