Strain identifier

BacDive ID: 5506

Type strain: Yes

Species: Elizabethkingia meningoseptica

Strain Designation: 14 (serotype A)

Strain history: CIP <- 1960, E.O. King, CDC: strain 14, Flavobacterium meningosepticum

NCBI tax ID(s): 1216967 (strain), 238 (species)

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General

@ref: 1230

BacDive-ID: 5506

DSM-Number: 2800

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped, antibiotic resistance, human pathogen

description: Elizabethkingia meningoseptica 14 is an obligate aerobe, mesophilic, Gram-negative human pathogen that has multiple antibiotic resistances and was isolated from cerebrospinal fluid of premature infant.

NCBI tax id

NCBI tax idMatching level
1216967strain
238species

strain history

@refhistory
1230<- NCTC <- E.O. King, 14 (serotype A)
365061960, E.O. King, CDC: strain 14, Flavobacterium meningosepticum
67770IAM 14198 <-- IFO 12535 <-- AJ 2517 <-- ATCC 13253 <-- E. O. King 14 (Group A).
120894CIP <- 1960, E.O. King, CDC: strain 14, Flavobacterium meningosepticum

doi: 10.13145/bacdive5506.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Weeksellaceae
  • genus: Elizabethkingia
  • species: Elizabethkingia meningoseptica
  • full scientific name: Elizabethkingia meningoseptica (King 1959) Kim et al. 2005

  • synonyms

    @refsynonym
    20215Flavobacterium meningosepticum
    20215Chryseobacterium meningosepticum

@ref: 1230

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Weeksellaceae

genus: Elizabethkingia

species: Elizabethkingia meningoseptica

full scientific name: Elizabethkingia meningoseptica (King 1959) Kim et al. 2005 emend. Nicholson et al. 2018

strain designation: 14 (serotype A)

type strain: yes

Morphology

cell morphology

  • @ref: 120894
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1230NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
36506MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120894CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
120894CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
1230positivegrowth37mesophilic
36506positivegrowth30mesophilic
67770positivegrowth37mesophilic
120894positivegrowth25-30mesophilic
120894nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120894
  • oxygen tolerance: obligate aerobe

antibiogram

  • @ref: 1230
  • medium: Mueller-Hinton Agar
  • Penicillin G: 0
  • oxacillin: 0
  • ampicillin: 0
  • ticarcillin: 10
  • mezlocillin: 14
  • cefalotin: 0
  • cefazolin: 0
  • cefotaxime: 8
  • aztreonam: 0
  • imipenem: 16
  • tetracycline: 12-14
  • chloramphenicol: 20-22
  • gentamycin: 0
  • amikacin: 10
  • vancomycin: 22
  • erythromycin: 12-14
  • lincomycin: 0
  • ofloxacin: 20
  • norfloxacin: 12
  • colistin: 0
  • pipemidic acid: 0
  • nitrofurantoin: 0
  • bacitracin: 0
  • polymyxin b: 0
  • kanamycin: 18
  • neomycin: 0
  • doxycycline: 28
  • ceftriaxone: 10
  • clindamycin: 28
  • fosfomycin: 0
  • moxifloxacin: 30-32
  • linezolid: 8
  • nystatin: 0
  • quinupristin/dalfopristin: 0
  • teicoplanin: 14
  • piperacillin/tazobactam: 30

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12089416947citrate-carbon source
1208944853esculin+hydrolysis
12089417632nitrate-reduction
12089416301nitrite+reduction
6837418257ornithine-degradation
6837429016arginine-hydrolysis
6837425094lysine-degradation
6837416199urea-hydrolysis
6837418403L-arabitol-builds acid from
6837418024D-galacturonic acid-builds acid from
68374Potassium 5-ketogluconate-builds acid from
6837416899D-mannitol-builds acid from
6837417306maltose-builds acid from
6837415963ribitol-builds acid from
6837418394palatinose-builds acid from
6837415792malonate-assimilation
6837427897tryptophan+energy source
6837417634D-glucose-builds acid from
6837417992sucrose-builds acid from
6837430849L-arabinose-builds acid from
6837418333D-arabitol-builds acid from
6837427082trehalose-builds acid from
6837462345L-rhamnose-builds acid from
6837417268myo-inositol-builds acid from
6837417057cellobiose-builds acid from
6837430911sorbitol-builds acid from

antibiotic resistance

  • @ref: 120894
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6837435581indoleyes
12089435581indoleyes

metabolite tests

  • @ref: 68374
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
68374L-aspartate arylamidase+3.4.11.21
68374alpha-maltosidase+
68374alpha-galactosidase+3.2.1.22
68374alpha-glucosidase+3.2.1.20
68374beta-galactosidase+3.2.1.23
68374N-acetyl-beta-glucosaminidase+3.2.1.52
68374beta-glucuronidase-3.2.1.31
68374beta-glucosidase+3.2.1.21
68374lipase+
68374urease-3.5.1.5
68374lysine decarboxylase-4.1.1.18
68374arginine dihydrolase-3.5.3.6
68374ornithine decarboxylase-4.1.1.17
120894oxidase+
120894beta-galactosidase+3.2.1.23
120894alcohol dehydrogenase-1.1.1.1
120894gelatinase+
120894amylase-
120894caseinase+3.4.21.50
120894catalase+1.11.1.6
120894tween esterase-
120894lecithinase-
120894lysine decarboxylase-4.1.1.18
120894ornithine decarboxylase-4.1.1.17
120894tryptophan deaminase-
120894urease-3.5.1.5

API ID32E

@refODCADH ArgLDC LysURELARLGAT5KGLIPRPbeta GLUMANMALADOPLEbeta GURMNTINDbeta NAGbeta GALGLUSACLARADARLalpha GLUalpha GALTRERHAINOCELSORalphaMALAspA
44136-------+++------+++----++-----++

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120894++-++----+++----+-+-----+------+-+--------------------+----+-+------------------------+--+++--+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateisolation date
1230cerebrospinal fluid of premature infantGeorgia, CDCUSAUSANorth America
44136Human cerebrospinal fluid,premature infantMassachusettsUSAUSANorth America1959
67770Spinal fluid from newborn
120894Human, Infant, cerebrospinal fluidMassachusettsUnited States of AmericaUSANorth America1949

isolation source categories

Cat1Cat2Cat3
#Infection#Patient
#Host Body Product#Fluids#Cerebrospinal fluid
#Host#Human#Child

taxonmaps

  • @ref: 69479
  • File name: preview.99_808.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_76;96_186;97_202;98_225;99_808&stattab=map
  • Last taxonomy: Elizabethkingia
  • 16S sequence: AY468445
  • Sequence Identity:
  • Total samples: 13141
  • soil counts: 1194
  • aquatic counts: 4420
  • animal counts: 6692
  • plant counts: 835

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
1230yesyes2Risk group (German classification)
1208942Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Chryseobacterium meningosepticum partial 16S rRNA gene, strain 13253TAJ7045401450ena238
20218Chryseobacterium meningosepticum strain ATCC 13253 16S ribosomal RNA gene, partial sequenceAY3365681450ena238
20218Chryseobacterium meningosepticum strain LMG 4027 16S ribosomal RNA gene, partial sequenceAY4684451452ena238
20218Elizabethkingia meningoseptica ATCC:13253 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceFJ4103863332ena238
20218Chryseobacterium meningosepticum 16S ribosomal RNA gene, partial sequenceM587761280ena238
20218Flavobacterium meningosepticum ATCC 13253 16S rRNA gene, partial sequenceU02896305ena238
20218Elizabethkingia meningoseptica strain BCRC 10677 16S-23S ribosomal RNA intergenic spacer, complete sequenceEU014552616ena238
20218Flavobacterium meningosepticum 16S ribosomal RNAD140181269ena238
20218Elizabethkingia meningoseptica gene for 16S rRNA, partial sequence, strain: NBRC 12535AB6802911442ena238

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Elizabethkingia meningoseptica NCTC10016GCA_900475375completencbi238
66792Elizabethkingia meningoseptica ATCC 13253 = NBRC 12535GCA_000401415contigncbi1216967
66792Elizabethkingia meningoseptica Elizabethkingia meningoseptica CCUG 214GCA_900092105contigncbi238
66792Elizabethkingia meningoseptica ATCC 13253 = NBRC 12535 [PRJNA199489]1216967.6wgspatric1216967
66792Elizabethkingia meningoseptica ATCC 13253 = NBRC 12535 [PRJNA205457]1216967.5wgspatric1216967
66792Elizabethkingia meningoseptica strain Elizabethkingia meningoseptica CCUG 214238.66wgspatric238
66792Elizabethkingia meningoseptica strain NCTC10016238.80completepatric238
66792Elizabethkingia meningoseptica ATCC 132532545824725draftimg1216967
66792Elizabethkingia meningoseptica NBRC 125352563367139draftimg1216967
66792Elizabethkingia meningoseptica Group A NCTC 100162823236545completeimg238
67770Elizabethkingia meningoseptica ATCC 13253 = NBRC 12535GCA_000367325contigncbi1216967

GC content

  • @ref: 1230
  • GC-content: 38.4

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno95.4no
gram-positiveno97.971no
anaerobicno99.578no
aerobicyes90.637no
halophileno94.639no
spore-formingno94.636no
thermophileno99.542yes
glucose-utilyes85.135no
motileno90.54no
glucose-fermentno92.177no

External links

@ref: 1230

culture collection no.: DSM 2800, ATCC 13253, IAM 14198, IFO 12535, NBRC 12535, NCTC 10016, CCUG 214, CIP 60.57, LMG 12279, JCM 21065, AJ 2517, CCM 2719, CCTM La 3011, CDC 14, CECT 447, GIFU 506, LMG 4027, PCM 2225, RIMD 0614002

straininfo link

  • @ref: 74991
  • straininfo: 13937

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism3761040Menaquinone (vitamin K2)-6 production by mutants of Flavobacterium meningosepticum.Tani Y, Asahi S, Yamada HJ Nutr Sci Vitaminol (Tokyo)10.3177/jnsv.32.1371986Amino Acids/metabolism, Carotenoids/biosynthesis, Drug Resistance, Microbial, Flavobacterium/drug effects/genetics/*metabolism, *Mutation, Naphthols/pharmacology, Potassium Cyanide/pharmacology, Vitamin K/*biosynthesisBiotechnology
Enzymology9683486Chemical structure of lipid A isolated from Flavobacterium meningosepticum lipopolysaccharide.Kato H, Haishima Y, Iida T, Tanaka A, Tanamoto KJ Bacteriol10.1128/JB.180.15.3891-3899.19981998Amides, Chromatography, Gas, Disaccharides/chemistry, Esters, Flavobacterium/*chemistry/immunology, Gas Chromatography-Mass Spectrometry, Lipid A/*chemistry/isolation & purification, Lipopolysaccharides/chemistry, Methylation, Models, Molecular, Nuclear Magnetic Resonance, BiomolecularPhylogeny
Metabolism10817691Carbapenemases of Chryseobacterium (Flavobacterium) meningosepticum: distribution of blaB and characterization of a novel metallo-beta-lactamase gene, blaB3, in the type strain, NCTC 10016.Woodford N, Palepou MF, Babini GS, Holmes B, Livermore DMAntimicrob Agents Chemother10.1128/AAC.44.6.1448-1452.20002000Amino Acid Sequence, *Bacterial Proteins, Flavobacterium/*enzymology/*genetics, *Genes, Bacterial, Metalloproteins/genetics/metabolism, Molecular Sequence Data, beta-Lactamases/*genetics/metabolismGenetics
Phylogeny15879269Transfer of Chryseobacterium meningosepticum and Chryseobacterium miricola to Elizabethkingia gen. nov. as Elizabethkingia meningoseptica comb. nov. and Elizabethkingia miricola comb. nov.Kim KK, Kim MK, Lim JH, Park HY, Lee STInt J Syst Evol Microbiol10.1099/ijs.0.63541-02005Bacterial Typing Techniques, Base Composition, Chryseobacterium/*classification/cytology/physiology, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Fatty Acids/analysis/isolation & purification, Flavobacteriaceae/*classification/cytology/physiology, Genes, Bacterial, Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAEnzymology
Pathogenicity19327918First isolation and identification of Elizabethkingia meningoseptica from cultured tiger frog, Rana tigerina rugulosa.Xie ZY, Zhou YC, Wang SF, Mei B, Xu XD, Wen WY, Feng YQVet Microbiol10.1016/j.vetmic.2009.02.0112009Animals, Anti-Bacterial Agents/pharmacology, Cataract/*veterinary, China, Chryseobacterium/drug effects/genetics/*isolation & purification/pathogenicity, Conjunctivitis/microbiology, Flavobacteriaceae Infections/genetics/*veterinary, Humans, Microbial Sensitivity Tests, Opportunistic Infections/microbiology, Ranidae/*microbiology, Torticollis/veterinary, VirulencePhylogeny
Phylogeny19819990Soonwooa buanensis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from seawater.Joung Y, Song J, Lee K, Oh HM, Joh K, Cho JCInt J Syst Evol Microbiol10.1099/ijs.0.017707-02009DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Flavobacteriaceae/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiologyMetabolism
Phylogeny21169462Elizabethkingia anophelis sp. nov., isolated from the midgut of the mosquito Anopheles gambiae.Kampfer P, Matthews H, Glaeser SP, Martin K, Lodders N, Faye IInt J Syst Evol Microbiol10.1099/ijs.0.026393-02010Animals, Anopheles/*microbiology, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Flavobacteriaceae/*classification/genetics/*isolation & purification/metabolism, Gastrointestinal Tract/microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/geneticsMetabolism
Enzymology21545730Biofilm formation and adherence characteristics of an Elizabethkingia meningoseptica isolate from Oreochromis mossambicus.Jacobs A, Chenia HYAnn Clin Microbiol Antimicrob10.1186/1476-0711-10-162011Animals, *Bacterial Adhesion, *Biofilms, Flavobacteriaceae/chemistry/genetics/*isolation & purification/*physiology, Hydrophobic and Hydrophilic Interactions, Tilapia/*microbiologyPhylogeny
Phylogeny25858248Elizabethkingia endophytica sp. nov., isolated from Zea mays and emended description of Elizabethkingia anophelisKampfer et al. 2011.Kampfer P, Busse HJ, McInroy JA, Glaeser SPInt J Syst Evol Microbiol10.1099/ijs.0.0002362015Alabama, Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacteriaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phosphatidylethanolamines/chemistry, *Phylogeny, Plant Stems/microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spermidine/analogs & derivatives/chemistry, Vitamin K 2/analogs & derivatives/chemistry, Zea mays/*microbiologyGenetics
Pathogenicity31120389Global Proteome Profiling Reveals Drug-Resistant Traits in Elizabethkingia meningoseptica: An Opportunistic Nosocomial Pathogen.Agrawal A, Ravikumar R, Varun CN, Kumar M, Chatterjee O, Advani J, Gopalakrishnan L, Nagaraj S, Mohanty V, Patil AH, Sreeramulu B, Malik A, Pinto SM, Prasad TSKOMICS10.1089/omi.2019.00392019Anti-Bacterial Agents/pharmacology, Chryseobacterium/*drug effects/*metabolism, Communicable Diseases/*metabolism, Humans, Microbial Sensitivity Tests, Proteomics/*methodsMetabolism
Phylogeny33835912Elizabethkingia argenteiflava sp. nov., isolated from the pod of soybean, Glycine max.Hwang JH, Kim J, Kim JH, Mo SInt J Syst Evol Microbiol10.1099/ijsem.0.0047672021Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacteriaceae/*classification/isolation & purification, Nucleic Acid Hybridization, Phosphatidylethanolamines/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Soybeans/*microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1230Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2800)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2800
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36506Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/9874
44136Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 214)https://www.ccug.se/strain?id=214
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68374Automatically annotated from API ID32E
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
74991Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13937.1StrainInfo: A central database for resolving microbial strain identifiers
120894Curators of the CIPCollection of Institut Pasteur (CIP 60.57)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2060.57