Strain identifier

BacDive ID: 5501

Type strain: Yes

Species: Cellulophaga baltica

Strain Designation: NN015840

Strain history: CIP <- 1999, P. Nielsen, Novo Nordisk, Denmark: strain NN015840 <- J.E. Johansen

NCBI tax ID(s): 76594 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 17745

BacDive-ID: 5501

DSM-Number: 24729

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped

description: Cellulophaga baltica NN015840 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from surface of brown alga Fucus serattus L..

NCBI tax id

  • NCBI tax id: 76594
  • Matching level: species

strain history

@refhistory
17745<- NCIMB <- J. E. Johansen
116720CIP <- 1999, P. Nielsen, Novo Nordisk, Denmark: strain NN015840 <- J.E. Johansen

doi: 10.13145/bacdive5501.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Cellulophaga
  • species: Cellulophaga baltica
  • full scientific name: Cellulophaga baltica Johansen et al. 1999

@ref: 17745

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Cellulophaga

species: Cellulophaga baltica

full scientific name: Cellulophaga baltica Johansen et al. 1999

strain designation: NN015840

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
43372negative2.2-4.5 µm0.6-0.8 µmrod-shapedno
69480negative99.85
116720negativerod-shapedno

colony morphology

@refincubation periodcolony sizecolony colorcolony shapemedium used
177451-2 days
433727 mmYellow with flame-like edgecircularTSA+20% sea salt
433720.5-1 mmpale yellowcircularCYT

pigmentation

  • @ref: 43372
  • production: no
  • name: Flexirubin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17745BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
39487Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
43372TSA+20% sea saltyes
43372CYTyes
116720CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13
116720CIP Medium 326yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=326

culture temp

@refgrowthtypetemperaturerange
17745positivegrowth28mesophilic
39487positivegrowth25mesophilic
43372positivemaximum32mesophilic
43372positiveoptimum25mesophilic
116720positivegrowth5-30
116720nogrowth37mesophilic
116720nogrowth41thermophilic
116720nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 43372
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.996

halophily

@refsaltgrowthtested relationconcentrationhalophily level
43372Marine saltspositivegrowth10-50 %
43372Marine saltspositiveoptimum20 %slightly halophilic
116720NaClpositivegrowth2-4 %
116720NaClnogrowth0 %
116720NaClnogrowth6 %
116720NaClnogrowth8 %
116720NaClnogrowth10 %

observation

  • @ref: 43372
  • observation: Colony morphology changes significantly depending on the temperature, substrate and salinity.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
43372167632-oxobutanoate+assimilation
43372309162-oxoglutarate-assimilation
43372286442-oxopentanoate-assimilation
433722509agar+hydrolysis
433722509agar+degradation
4337258187alginate+degradation
4337217665alpha-D-glucose 6-phosphate+assimilation
4337236219alpha-lactose-assimilation
433728295beta-hydroxybutyrate+assimilation
433723435carrageenan+degradation
43372casein+hydrolysis
43372casein+degradation
4337218333D-arabitol+assimilation
4337215824D-fructose+assimilation
4337212936D-galactose+assimilation
4337218024D-galacturonic acid-assimilation
433728391D-gluconate-assimilation
4337214314D-glucose 6-phosphate-assimilation
4337216899D-mannitol-assimilation
4337216024D-mannose+assimilation
4337216634raffinose-assimilation
4337217924D-sorbitol-assimilation
4337216551D-trehalose+assimilation
4337223652dextrin-assimilation
433724767elastin+degradation
4337215740formate-assimilation
43372167244-hydroxybutyrate-assimilation
433725291gelatin+degradation
4337228066gentiobiose+assimilation
4337229042glucose 1-phosphate-assimilation
4337232323glucuronamide+assimilation
4337228087glycogen-assimilation
4337285249hydroxyethylcellulose+degradation
4337230849L-arabinose-assimilation
4337218287L-fucose+assimilation
4337229985L-glutamate+assimilation
4337215729L-ornithine-assimilation
4337217203L-proline-assimilation
4337262345L-rhamnose-assimilation
4337216857L-threonine-assimilation
433726359lactulose-assimilation
4337215792malonate-assimilation
4337217306maltose-assimilation
4337237657methyl D-glucoside-assimilation
4337251850methyl pyruvate+assimilation
4337275146monomethyl succinate-assimilation
4337217268myo-inositol+assimilation
4337217272propionate-assimilation
4337233951psicose-assimilation
4337228017starch+hydrolysis
4337228017starch+degradation
4337230031succinate-assimilation
4337217992sucrose+assimilation
4337232528turanose-assimilation
4337253423tween 40-assimilation
1167204853esculin-hydrolysis
116720606565hippurate-hydrolysis
11672017632nitrate+reduction
11672016301nitrite-reduction
11672035020tributyrin-hydrolysis
11672015792malonate-assimilation

metabolite production

  • @ref: 116720
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11672015688acetoin+
11672017234glucose-

enzymes

@refvalueactivityec
43372catalase+1.11.1.6
43372cytochrome oxidase-1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
116720oxidase-
116720beta-galactosidase+3.2.1.23
116720alcohol dehydrogenase-1.1.1.1
116720gelatinase+
116720amylase+
116720caseinase+3.4.21.50
116720catalase+1.11.1.6
116720tween esterase-
116720gamma-glutamyltransferase+2.3.2.2
116720lecithinase-
116720lipase-
116720lysine decarboxylase-4.1.1.18
116720ornithine decarboxylase-4.1.1.17
116720phenylalanine ammonia-lyase-4.3.1.24
116720protease+
116720tryptophan deaminase-
116720urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116720-+-+-+----++-+-+++--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116720---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentlatitudelongitudeisolation date
17745surface of brown alga Fucus serattus L.Fucus serattusBaltic SeaDenmarkDNKEurope
43372surface of the brown alga Fucus serratusBornholm (Island in Baltic Sea)DenmarkDNKEurope5514
116720Surface of brown alga Fucus serratus L.Baltic SeaDenmarkDNKEurope1995

isolation source categories

Cat1Cat2Cat3
#Host#Algae#Brown Algae
#Environmental#Aquatic#Marine
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_5415.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_102;96_900;97_2087;98_4039;99_5415&stattab=map
  • Last taxonomy: Cellulophaga
  • 16S sequence: AJ005972
  • Sequence Identity:
  • Total samples: 652
  • soil counts: 22
  • aquatic counts: 605
  • animal counts: 25

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
177451Risk group (German classification)
1167201Risk group (French classification)

Sequence information

16S sequences

  • @ref: 17745
  • description: Cytophaga baltica 16S rRNA gene, type strain NN015840, partial
  • accession: AJ005972
  • length: 1474
  • database: ena
  • NCBI tax ID: 76594

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Cellulophaga baltica DSM 24729GCA_900102165scaffoldncbi76594
66792Cellulophaga baltica strain DSM 2472976594.4wgspatric76594
66792Cellulophaga baltica DSM 247292622736528draftimg76594

GC content

  • @ref: 43372
  • GC-content: 33
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno83.794no
flagellatedno94.888no
gram-positiveno97.873no
anaerobicno99.674yes
aerobicyes91.33yes
halophileno78.119no
spore-formingno95.193no
glucose-fermentno89.469no
thermophileno99.582yes
glucose-utilyes89.108no

External links

@ref: 17745

culture collection no.: DSM 24729, ATCC 700862, CIP 106307, LMG 18535, NCIMB 13610

straininfo link

  • @ref: 74986
  • straininfo: 12967

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10425785Description of Cellulophaga baltica gen. nov., sp. nov. and Cellulophaga fucicola gen. nov., sp. nov. and reclassification of [Cytophaga] lytica to Cellulophaga lytica gen. nov., comb. nov.Johansen JE, Nielsen P, Sjoholm CInt J Syst Bacteriol10.1099/00207713-49-3-12311999Bacteria/*classification/cytology/genetics/isolation & purification, Bacteroides/classification/genetics, Base Composition, Cytophaga/*classification/cytology/genetics, DNA, Bacterial/chemistry/genetics/isolation & purification, DNA, Ribosomal/chemistry/genetics, Denmark, Flavobacterium/classification/genetics, Molecular Sequence Data, Nucleic Acid Hybridization, Phaeophyta/*microbiology, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Water MicrobiologyEnzymology
36239409Elucidation of the O-antigen structure of Escherichia coli O93 and characterization of its biosynthetic genes.Furevi A, Stahle J, Muheim C, Gkotzis S, Daley DO, Udekwu KI, Widmalm GGlycobiology10.1093/glycob/cwac0692022

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
17745Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24729)https://www.dsmz.de/collection/catalogue/details/culture/DSM-24729
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39487Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18424
43372Jens E. Johansen, Preben Nielsen, Carsten Sjraholm10.1099/00207713-49-3-1231Description of Cellulophaga baltica gen. nov., sp. nov. and Cellulophaga fucicola gen. nov., sp. nov. and reclassification of Cytophaga Iytica to Cellulophaga lytica gen. nov., comb. nov.IJSB 49: 1231-1240 199910425785
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
74986Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID12967.1StrainInfo: A central database for resolving microbial strain identifiers
116720Curators of the CIPCollection of Institut Pasteur (CIP 106307)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106307