Strain identifier

BacDive ID: 5500

Type strain: Yes

Species: Cellulophaga algicola

Strain Designation: IC166

Strain history: CIP <- 2002, J. Bowman, Tasmania Univ., Tasmania, Australia: strain ACAM 630

NCBI tax ID(s): 688270 (strain), 59600 (species)

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General

@ref: 5265

BacDive-ID: 5500

DSM-Number: 14237

keywords: genome sequence, 16S sequence, Bacteria, aerobe, chemoorganotroph, psychrophilic, Gram-negative, motile, rod-shaped

description: Cellulophaga algicola IC166 is an aerobe, chemoorganotroph, psychrophilic bacterium that was isolated from sea ice diatons, macrophyte surfaces.

NCBI tax id

NCBI tax idMatching level
688270strain
59600species

strain history

@refhistory
5265<- J. P. Bowman
407102002, J. Bowman, Tasmania Univ., Tasmania, Australia: strain ACAM 630
116223CIP <- 2002, J. Bowman, Tasmania Univ., Tasmania, Australia: strain ACAM 630

doi: 10.13145/bacdive5500.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Cellulophaga
  • species: Cellulophaga algicola
  • full scientific name: Cellulophaga algicola Bowman 2000

@ref: 5265

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Cellulophaga

species: Cellulophaga algicola

full scientific name: Cellulophaga algicola Bowman 2000 emend. Hahnke et al. 2016

strain designation: IC166

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
43416negative1.5-4 µm0.4-0.5 µmrod-shapedyes
69480negative99.976
116223negativerod-shapedno

colony morphology

  • @ref: 43416
  • colony color: yellow-orange
  • medium used: marine 2216 agar

pigmentation

  • @ref: 43416
  • production: no
  • name: Flexirubin

multimedia

  • @ref: 66793
  • multimedia content: EM_DSM_14237_1.jpg
  • caption: electron microscopic image
  • intellectual property rights: © HZI/Manfred Rohde

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5265BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
40710Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
43416marine 2216 agaryes
116223CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
5265positivegrowth10-20psychrophilic
40710positivegrowth20psychrophilic
43416positiveoptimum20-25
43416positiveoptimum15-20psychrophilic
43416positivegrowth-2-28
116223positivegrowth5-25psychrophilic
116223nogrowth30mesophilic
116223nogrowth37mesophilic
116223nogrowth41thermophilic
116223nogrowth45thermophilic

culture pH

  • @ref: 43416
  • ability: positive
  • type: optimum
  • pH: 7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 43416
  • oxygen tolerance: aerobe

nutrition type

  • @ref: 43416
  • type: chemoorganotroph

spore formation

@refspore formationconfidence
69481no100
69480no99.994

halophily

@refsaltgrowthtested relationconcentration
43416NaClpositivegrowth0.5-10 %
43416NaClpositiveoptimum2 %
116223NaClnogrowth0 %
116223NaClnogrowth2 %
116223NaClnogrowth4 %
116223NaClnogrowth6 %
116223NaClnogrowth8 %
116223NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
434162509agar+hydrolysis
4341685146carboxymethylcellulose+hydrolysis
43416casein+hydrolysis
4341617057cellobiose+builds acid from
4341615824D-fructose+builds acid from
4341612936D-galactose+builds acid from
4341617634D-glucose+builds acid from
4341652071dextran-hydrolysis
434165291gelatin+hydrolysis
4341617895L-tyrosine+hydrolysis
4341617716lactose+builds acid from
4341629864mannitol+builds acid from
4341628017starch+hydrolysis
4341617992sucrose+builds acid from
4341635020tributyrin+hydrolysis
4341653426tween 80+hydrolysis
4341627226uric acid-hydrolysis
4341615318xanthine-hydrolysis
1162234853esculin-hydrolysis
116223606565hippurate-hydrolysis
11622317632nitrate+reduction
11622316301nitrite-reduction
11622315792malonate-assimilation

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitiveis resistant
4341628971ampicillinyesyes
434163393carbenicillinyesyes
4341617076streptomycinyesyes
4341627902tetracyclineyesyes

metabolite production

@refChebi-IDmetaboliteproduction
4341616136hydrogen sulfideno
4341635581indoleno
11622335581indoleno

metabolite tests

  • @ref: 116223
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
116223oxidase+
116223beta-galactosidase+3.2.1.23
116223alcohol dehydrogenase-1.1.1.1
116223gelatinase+/-
116223amylase+
116223caseinase+3.4.21.50
116223catalase+1.11.1.6
116223tween esterase+
116223gamma-glutamyltransferase+2.3.2.2
116223lecithinase-
116223lipase-
116223lysine decarboxylase-4.1.1.18
116223ornithine decarboxylase-4.1.1.17
116223phenylalanine ammonia-lyase-4.3.1.24
116223protease+
116223tryptophan deaminase-
116223urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116223-+++-++---+++--+++--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116223-----------------++--------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcontinentcountryorigin.countryisolation date
5265sea ice diatons, macrophyte surfacesPrydz BayAustralia and Oceania
116223Sea ice colonial diatom MelosiraAntarcticaAntarcticaATA1996

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Ice
#Environmental#Aquatic#Marine
#Host#Other

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
52651Risk group (German classification)
1162231Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5265
  • description: Melosira colonizing bacterium IC166 16S ribosomal RNA gene, partial sequence
  • accession: AF001366
  • length: 1453
  • database: ena
  • NCBI tax ID: 688270

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Cellulophaga algicola DSM 14237GCA_000186265completencbi688270
66792Cellulophaga algicola DSM 14237688270.3completepatric688270
66792Cellulophaga algicola IC166, DSM 14237649633031completeimg688270

GC content

@refGC-contentmethod
526536-38thermal denaturation, midpoint method (Tm)
4341633.77
526533.8sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno80.862yes
flagellatedno94.581no
gram-positiveno97.486no
anaerobicno99.714yes
aerobicyes90.279yes
halophileno70.384no
spore-formingno94.64no
glucose-utilyes91.766no
thermophileno99.228yes
glucose-fermentno88.091no

External links

@ref: 5265

culture collection no.: DSM 14237, ACAM 630, CIP 107446, LMG 21425

straininfo link

  • @ref: 74985
  • straininfo: 99981

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11034497Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.Bowman JPInt J Syst Evol Microbiol10.1099/00207713-50-5-18612000Antarctic Regions, Bacteroidetes/chemistry/*classification/genetics, Base Composition, Cytophaga/chemistry/*classification/genetics, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Eukaryota/*microbiology, Fatty Acids/analysis, Genotype, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics
Genetics21475589Complete genome sequence of Cellulophaga algicola type strain (IC166).Abt B, Lu M, Misra M, Han C, Nolan M, Lucas S, Hammon N, Deshpande S, Cheng JF, Tapia R, Goodwin L, Pitluck S, Liolios K, Pagani I, Ivanova N, Mavromatis K, Ovchinikova G, Pati A, Chen A, Palaniappan K, Land M, Hauser L, Chang YJ, Jeffries CD, Detter JC, Brambilla E, Rohde M, Tindall BJ, Goker M, Woyke T, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HP, Lapidus AStand Genomic Sci10.4056/sigs.15438452011
Metabolism26463370Expression and characterization of a cold-adapted, thermotolerant and denaturant-stable GH5 endoglucanase Celal_2753 that withstands boiling from the psychrophilic bacterium Cellulophaga algicola IC166(T).Wang Y, Yu W, Han FBiotechnol Lett10.1007/s10529-015-1971-52015Carboxymethylcellulose Sodium/metabolism, Cellulase/chemistry/*isolation & purification/*metabolism, Cold Temperature, Detergents/metabolism, Electrophoresis, Polyacrylamide Gel, Enzyme Activators/metabolism, Enzyme Stability, Flavobacteriaceae/*enzymology, Hot Temperature, Hydrogen-Ion Concentration, Hydrolysis, Molecular Weight, Potassium Chloride/metabolism, Protein Denaturation/*drug effects/*radiation effects, Sodium Chloride/metabolismEnzymology
Enzymology31052274Biochemical Characterization of a New beta-Agarase from Cellulophaga Algicola.Han Z, Zhang Y, Yang JInt J Mol Sci10.3390/ijms200921432019Bacterial Proteins/chemistry/*metabolism, Catalytic Domain, Enzyme Stability, Flavobacteriaceae/*enzymology, Glycoside Hydrolases/chemistry/*metabolism, Protein Binding, Substrate SpecificityMetabolism
Metabolism31325715Chromatographic analysis of alginate degradation by five recombinant alginate lyases from Cellulophaga algicola DSM 14237.Fischer A, Wefers DFood Chem10.1016/j.foodchem.2019.1251422019Alginates/chemistry/*metabolism, Bacterial Proteins/genetics/metabolism, Chromatography, Gel/*methods, Chromatography, Ion Exchange/*methods, Cloning, Molecular, Flavobacteriaceae/*enzymology/genetics, Hexuronic Acids/metabolism, Hydrolysis, Polysaccharide-Lyases/genetics/*metabolism, Recombinant Proteins/genetics/metabolism, Substrate SpecificityEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5265Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14237)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14237
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
40710Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4882
43416Birte Abt, Megan Lu, Monica Misra, Cliff Han, Matt Nolan, Susan Lucas, Nancy Hammon, Shweta Deshpande, Jan-Fang Cheng , Roxane Tapia, Lynne Goodwin, Sam Pitluck, Konstantinos Liolios, Ioanna Pagani, Natalia Ivanova, Konstantinos Mavromatis, Galina Ovchinikova, Amrita Pati, Amy Chen, Krishna Palaniappan, Miriam Land, Loren Hauser, Yun-Juan Chang, Cynthia D. Jeffries, John C. Detter, Evelyne Brambilla, Manfred Rohde, Brian J. Tindall, Markus Göker, Tanja Woyke, James Bristow, Jonathan A. Eisen, Victor Markowitz, Philip Hugenholtz, Nikos C. Kyrpides, Hans-Peter Klenk, Alla Lapidus10.4056/sigs.1543845Complete genome sequence of Cellulophaga algicola type strain (IC166)Stand Genomic Sci 4: 72-80 201121475589
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
74985Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID99981.1StrainInfo: A central database for resolving microbial strain identifiers
116223Curators of the CIPCollection of Institut Pasteur (CIP 107446)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107446