Strain identifier
BacDive ID: 5469
Type strain: 
Species: Thermonema rossianum
Strain Designation: NR-27
Strain history: <- M.S. Da Costa, University of Coimbra
NCBI tax ID(s): 1313301 (strain), 55505 (species)
version 8.1 (current version)
General
@ref: 3889
BacDive-ID: 5469
DSM-Number: 10300
keywords: genome sequence, 16S sequence, Bacteria, thermophilic, Gram-negative
description: Thermonema rossianum NR-27 is a thermophilic, Gram-negative bacterium that was isolated from saline hot springs.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 55505 | species |
| 1313301 | strain |
strain history
- @ref: 3889
- history: <- M.S. Da Costa, University of Coimbra
doi: 10.13145/bacdive5469.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacteroidota
- domain: Bacteria
- phylum: Bacteroidota
- class: Cytophagia
- order: Cytophagales
- family: Thermonemataceae
- genus: Thermonema
- species: Thermonema rossianum
- full scientific name: Thermonema rossianum Nobre et al. 1997
@ref: 3889
domain: Bacteria
phylum: Bacteroidetes
class: Cytophagia
order: Cytophagales
family: Thermonemataceae
genus: Thermonema
species: Thermonema rossianum
full scientific name: Thermonema rossianum Nobre et al. 1997 emend. Hahnke et al. 2016
strain designation: NR-27
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: negative
- confidence: 100
Culture and growth conditions
culture medium
- @ref: 3889
- name: THERMUS 162 MEDIUM (DSMZ Medium 878)
- growth: yes
- link: https://mediadive.dsmz.de/medium/878
- composition: Name: THERMUS 162 MEDIUM (DSMZ Medium 878) Composition: Agar 28.0 g/l Na2HPO4 x 12 H2O 4.3 g/l Yeast extract 1.0 g/l Tryptone 1.0 g/l KH2PO4 0.544 g/l MgCl2 x 6 H2O 0.2 g/l Nitrilotriacetic acid 0.1 g/l CaSO4 x 2 H2O 0.04 g/l Fe(III) citrate 0.00122472 g/l MnSO4 x H2O 0.00114 g/l ZnSO4 x 7 H2O 0.00025 g/l H3BO3 0.00025 g/l CoCl2 x 6 H2O 2.25e-05 g/l CuSO4 x 5 H2O 1.25e-05 g/l Na2MoO4 x 2 H2O 1.25e-05 g/l H2SO4 Distilled water
culture temp
- @ref: 3889
- growth: positive
- type: growth
- temperature: 50
- range: thermophilic
Physiology and metabolism
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.993 |
Isolation, sampling and environmental information
isolation
- @ref: 3889
- sample type: saline hot springs
- geographic location: Bay of Naples
- country: Italy
- origin.country: ITA
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Thermal spring |
| #Condition | #Saline | |
| #Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_62819.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6181;96_27429;97_34504;98_44485;99_62819&stattab=map
- Last taxonomy: Thermonema rossianum subclade
- 16S sequence: Y08956
- Sequence Identity:
- Total samples: 26
- soil counts: 2
- aquatic counts: 23
- animal counts: 1
Safety information
risk assessment
- @ref: 3889
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 3889
- description: T.rossianum 16S rRNA gene (strain NR-27T)
- accession: Y08956
- length: 1465
- database: ena
- NCBI tax ID: 55505
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Thermonema rossianum DSM 10300 | GCA_000426825 | scaffold | ncbi | 1313301 |
| 66792 | Thermonema rossianum DSM 10300 | 1313301.3 | wgs | patric | 1313301 |
| 66792 | Thermonema rossianum DSM 10300 | 2523533595 | draft | img | 1313301 |
GC content
- @ref: 3889
- GC-content: 48.8
- method: sequence analysis
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| motile | no | 88.42 | no |
| flagellated | no | 95.773 | no |
| gram-positive | no | 98.838 | no |
| anaerobic | no | 97.028 | no |
| halophile | no | 71.567 | no |
| spore-forming | no | 94.594 | no |
| glucose-util | yes | 66.968 | no |
| aerobic | yes | 75.19 | no |
| thermophile | yes | 72.251 | yes |
| glucose-ferment | no | 90.296 | no |
External links
@ref: 3889
culture collection no.: DSM 10300
straininfo link
- @ref: 74957
- straininfo: 48799
literature
- topic: Phylogeny
- Pubmed-ID: 8995813
- title: Thermonema rossianum sp. nov., a new thermophilic and slightly halophilic species from saline hot springs in Naples, Italy.
- authors: Tenreiro S, Nobre MF, Rainey FA, Miguel C, da Costa MS
- journal: Int J Syst Bacteriol
- DOI: 10.1099/00207713-47-1-122
- year: 1997
- mesh: Bacteria, Aerobic/chemistry/*classification/genetics/growth & development, Bacteriological Techniques, Base Composition, Culture Media/metabolism, Fatty Acids/analysis, Hot Temperature, Lipids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, Sodium Chloride/metabolism, Vitamin K/analysis
- topic2: Metabolism
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 3889 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10300) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-10300 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |
| 74957 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID48799.1 | StrainInfo: A central database for resolving microbial strain identifiers |