Strain identifier

BacDive ID: 5433

Type strain: No

Species: Eubacterium sp.

Strain Designation: 32

Strain history: STAFF 1028 <-- ATCC 10825 <-- H. A. Barker 32 of group A2.

NCBI tax ID(s): 142586 (species)

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General

@ref: 8889

BacDive-ID: 5433

DSM-Number: 20517

keywords: 16S sequence, Bacteria, anaerobe, mesophilic

description: Eubacterium sp. 32 is an anaerobe, mesophilic bacterium that was isolated from Intestinal contents of white rats.

NCBI tax id

  • NCBI tax id: 142586
  • Matching level: species

strain history

@refhistory
8889<- ATCC <- H.A. Barker, 32 (Butyribacterium rettgeri)
67770STAFF 1028 <-- ATCC 10825 <-- H. A. Barker 32 of group A2.

doi: 10.13145/bacdive5433.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Eubacteriaceae
  • genus: Eubacterium
  • species: Eubacterium sp.
  • full scientific name: Eubacterium Prévot 1938 (Approved Lists 1980)

@ref: 8889

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Eubacteriaceae

genus: Eubacterium

species: Eubacterium sp.

full scientific name: Eubacterium sp.

strain designation: 32

type strain: no

Culture and growth conditions

culture medium

  • @ref: 8889
  • name: PYG MEDIUM (MODIFIED) (DSMZ Medium 104)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/104
  • composition: Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

culture temp

@refgrowthtypetemperaturerange
8889positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 8889
  • oxygen tolerance: anaerobe

murein

  • @ref: 8889
  • murein short key: B04
  • type: B2alpha {L-Ser} [L-Orn] D-Glu-D-Lys(D-Orn)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate+degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase+3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase+4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
8889------+/------+------+---------

Isolation, sampling and environmental information

isolation

  • @ref: 67770
  • sample type: Intestinal contents of white rats

taxonmaps

  • @ref: 69479
  • File name: preview.99_2312.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_30;96_53;97_56;98_1804;99_2312&stattab=map
  • Last taxonomy: Eubacterium maltosivorans subclade
  • 16S sequence: LC145549
  • Sequence Identity:
  • Total samples: 12960
  • soil counts: 159
  • aquatic counts: 925
  • animal counts: 11759
  • plant counts: 117

Sequence information

16S sequences

  • @ref: 67770
  • description: Eubacterium limosum gene for 16S ribosomal RNA, partial sequence, strain: JCM 9976
  • accession: LC145549
  • length: 1482
  • database: ena
  • NCBI tax ID: 1736

GC content

  • @ref: 8889
  • GC-content: 48.3

External links

@ref: 8889

culture collection no.: DSM 20517, ATCC 10825, NCIB 9554, NCTC 10469, JCM 9976, KCTC 2487, KCTC 5004, NCIMB 9554

straininfo link

  • @ref: 74921
  • straininfo: 35290

Reference

@idauthorscataloguedoi/urltitle
8889Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20517)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20517
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
74921Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID35290.1StrainInfo: A central database for resolving microbial strain identifiers