Strain identifier

BacDive ID: 5430

Type strain: No

Species: Eubacterium limosum

Strain Designation: 2A

Strain history: DSM 2593 <-- E. Naumann <-- R. Walther 2A.

NCBI tax ID(s): 1736 (species)

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General

@ref: 1144

BacDive-ID: 5430

DSM-Number: 2593

keywords: 16S sequence, Bacteria, anaerobe, mesophilic

description: Eubacterium limosum 2A is an anaerobe, mesophilic bacterium that was isolated from river mud.

NCBI tax id

  • NCBI tax id: 1736
  • Matching level: species

strain history

@refhistory
1144<- E. Naumann <- R. Walther, 2A
67770DSM 2593 <-- E. Naumann <-- R. Walther 2A.

doi: 10.13145/bacdive5430.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Eubacteriaceae
  • genus: Eubacterium
  • species: Eubacterium limosum
  • full scientific name: Eubacterium limosum (Eggerth 1935) Prévot 1938 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Bacteroides limosus
    20215Butyribacterium limosum

@ref: 1144

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Eubacteriaceae

genus: Eubacterium

species: Eubacterium limosum

full scientific name: Eubacterium limosum (Eggerth 1935) Prévot 1938

strain designation: 2A

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1144METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a)yeshttps://mediadive.dsmz.de/medium/120aName: METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a) Composition: Methanol 15.4688 g/l NaCl 2.19727 g/l Yeast extract 1.95312 g/l Casitone 1.95312 g/l NaHCO3 0.830078 g/l NH4Cl 0.488281 g/l MgSO4 x 7 H2O 0.488281 g/l K2HPO4 0.341797 g/l Na2S x 9 H2O 0.292969 g/l L-Cysteine HCl x H2O 0.292969 g/l CaCl2 x 2 H2O 0.244141 g/l KH2PO4 0.224609 g/l HCl 0.00244141 g/l FeSO4 x 7 H2O 0.00195312 g/l FeCl2 x 4 H2O 0.00146484 g/l Sodium resazurin 0.000488281 g/l CoCl2 x 6 H2O 0.000185547 g/l Pyridoxine hydrochloride 9.76563e-05 g/l MnCl2 x 4 H2O 9.76563e-05 g/l ZnCl2 6.83594e-05 g/l Thiamine HCl 4.88281e-05 g/l Riboflavin 4.88281e-05 g/l Calcium D-(+)-pantothenate 4.88281e-05 g/l Nicotinic acid 4.88281e-05 g/l p-Aminobenzoic acid 4.88281e-05 g/l (DL)-alpha-Lipoic acid 4.88281e-05 g/l Na2MoO4 x 2 H2O 3.51562e-05 g/l NiCl2 x 6 H2O 2.34375e-05 g/l Folic acid 1.95312e-05 g/l Biotin 1.95312e-05 g/l H3BO3 5.85938e-06 g/l CuCl2 x 2 H2O 1.95313e-06 g/l Vitamin B12 9.76563e-07 g/l H2SO4 Distilled water
1144CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)yeshttps://mediadive.dsmz.de/medium/110Name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l D-Glucose 4.0 g/l Starch 1.0 g/l Maltose 1.0 g/l Cellobiose 1.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Sodium resazurin 0.0005 g/l Vitamin K1 NaOH Distilled water
1144CHOPPED MEAT MEDIUM (DSMZ Medium 78)yeshttps://mediadive.dsmz.de/medium/78Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water

culture temp

@refgrowthtypetemperature
1144positivegrowth37
67770positivegrowth37

Physiology and metabolism

oxygen tolerance

  • @ref: 1144
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
1144river mudGöttingen, LeineGermanyDEUEurope
46529River mudGöttingen,LeineGermanyDEUEurope
67770River mud

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Mud (Sludge)
#Environmental#Aquatic#River (Creek)

taxonmaps

  • @ref: 69479
  • File name: preview.99_67.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_30;96_53;97_56;98_61;99_67&stattab=map
  • Last taxonomy: Eubacterium
  • 16S sequence: LC480790
  • Sequence Identity:
  • Total samples: 42112
  • soil counts: 550
  • aquatic counts: 2430
  • animal counts: 38820
  • plant counts: 312

Safety information

risk assessment

  • @ref: 1144
  • pathogenicity human: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 67770
  • description: Eubacterium limosum JCM 10283 gene for 16S ribosomal RNA, partial sequence
  • accession: LC480790
  • length: 1463
  • database: nuccore
  • NCBI tax ID: 1736

GC content

  • @ref: 1144
  • GC-content: 46.4

External links

@ref: 1144

culture collection no.: DSM 2593, JCM 10283, CCUG 16794

straininfo link

  • @ref: 74918
  • straininfo: 47635

Reference

@idauthorscataloguedoi/urltitle
1144Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2593)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2593
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
46529Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 16794)https://www.ccug.se/strain?id=16794
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
74918Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47635.1StrainInfo: A central database for resolving microbial strain identifiers