Strain identifier

BacDive ID: 5427

Type strain: Yes

Species: Anaerostipes hadrus

Strain Designation: VP 82-52

Strain history: <- DSM <- ATCC <- LV Holdeman, VPI 82-52

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General

@ref: 1356

BacDive-ID: 5427

DSM-Number: 3319

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, rod-shaped

description: Anaerostipes hadrus VP 82-52 is an anaerobe, mesophilic, rod-shaped bacterium that was isolated from human faeces.

NCBI tax id

NCBI tax idMatching level
649757strain
649756species

strain history

@refhistory
1356<- ATCC <- L.V. Holdeman, VPI 82-52
67771<- DSM <- ATCC <- LV Holdeman, VPI 82-52

doi: 10.13145/bacdive5427.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Anaerostipes
  • species: Anaerostipes hadrus
  • full scientific name: Anaerostipes hadrus (Moore et al. 1976) Allen-Vercoe et al. 2013
  • synonyms

    • @ref: 20215
    • synonym: Eubacterium hadrum

@ref: 1356

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Lachnospiraceae

genus: Anaerostipes

species: Anaerostipes hadrus

full scientific name: Anaerostipes hadrus (Moore et al. 1976) Allen-Vercoe et al. 2013

strain designation: VP 82-52

type strain: yes

Morphology

cell morphology

@refcell shapemotilitygram stain
67771rod-shapedno
67771positive

Culture and growth conditions

culture medium

  • @ref: 1356
  • name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/110
  • composition: Name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l D-Glucose 4.0 g/l Starch 1.0 g/l Maltose 1.0 g/l Cellobiose 1.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Sodium resazurin 0.0005 g/l Vitamin K1 NaOH Distilled water

culture temp

@refgrowthtypetemperaturerange
1356positivegrowth37mesophilic
67771positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
1356anaerobe
67771anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate+
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCPALArgAProALGAPheALeuAPyrATyrAAlaAHisAGGASerA
1356-+/--++---------------------

Isolation, sampling and environmental information

isolation

@refsample type
1356human faeces
67771From human faeces

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Gastrointestinal tract#Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_2505.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_33;96_1350;97_1603;98_1949;99_2505&stattab=map
  • Last taxonomy: Anaerostipes hadrus subclade
  • 16S sequence: FR749934
  • Sequence Identity:
  • Total samples: 55629
  • soil counts: 188
  • aquatic counts: 983
  • animal counts: 54372
  • plant counts: 86

Safety information

risk assessment

  • @ref: 1356
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Eubacterium hadrum partial 16S rRNA gene, type strain DSM 3319T, clone 1FR7499321616ena649757
20218Eubacterium hadrum partial 16S rRNA gene, type strain DSM 3319T, clone 2FR7499331618ena649757
20218Eubacterium hadrum partial 16S rRNA gene, type strain DSM 3319T, clone 3FR7499341515ena649757

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Anaerostipes hadrus DSM 3319649757.3wgspatric649757
66792Anaerostipes hadrus comb. nov. VPI 82-52, DSM 33192534681714draftimg649757
67771Anaerostipes hadrus ATCC 29173 = JCM 17467 DSM 3319GCA_000332875scaffoldncbi649757

GC content

  • @ref: 1356
  • GC-content: 32.0

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveyes89.407no
anaerobicyes99.118yes
halophileno63.612no
spore-formingno63.774no
glucose-utilyes93.007no
thermophileno97.098yes
flagellatedno92.726no
aerobicno98.8yes
motileno92.267yes
glucose-fermentyes68.798no

External links

@ref: 1356

culture collection no.: DSM 3319, ATCC 29173, KCTC 15606, VPI 82-52, JCM 9980, KCTC 15009

straininfo link

  • @ref: 74915
  • straininfo: 39388

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny22982042Anaerostipes hadrus comb. nov., a dominant species within the human colonic microbiota; reclassification of Eubacterium hadrum Moore et al. 1976.Allen-Vercoe E, Daigneault M, White A, Panaccione R, Duncan SH, Flint HJ, O'Neal L, Lawson PAAnaerobe10.1016/j.anaerobe.2012.09.0022012Bacterial Typing Techniques, Butyrates/metabolism, Cluster Analysis, Colon/*microbiology, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Female, Genotype, Gram-Positive Bacteria/*classification/genetics/*isolation & purification/physiology, Humans, Middle Aged, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics
Phylogeny30351260Reclassification of Eubacterium hallii as Anaerobutyricum hallii gen. nov., comb. nov., and description of Anaerobutyricum soehngenii sp. nov., a butyrate and propionate-producing bacterium from infant faeces.Shetty SA, Zuffa S, Bui TPN, Aalvink S, Smidt H, De Vos WMInt J Syst Evol Microbiol10.1099/ijsem.0.0030412018Bacterial Typing Techniques, Base Composition, Butyrates/metabolism, DNA, Bacterial/genetics, Eubacterium/*classification/metabolism, Fatty Acids/chemistry, Feces/*microbiology, Humans, Infant, Nucleic Acid Hybridization, Phosphatidylethanolamines/chemistry, Phosphatidylglycerols/chemistry, *Phylogeny, Propionates/metabolism, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAMetabolism
Phylogeny34491487Description of Anaerostipes faecalis sp. nov., a new segmented filamentous bacterium isolated from swine faeces.Choi SH, Choi JY, Park JE, Kim JS, Kang SW, Lee J, Lee MK, Lee JS, Lee JH, Jung H, Hur TY, Kim HB, Lee JH, Kim JK, Hong Y, Park SHAntonie Van Leeuwenhoek10.1007/s10482-021-01646-z2021Animals, Bacterial Typing Techniques, Clostridiales, DNA, Bacterial/genetics, *Fatty Acids/analysis, Feces, *Phospholipids, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, SwineTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1356Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3319)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3319
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
74915Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID39388.1StrainInfo: A central database for resolving microbial strain identifiers