Strain identifier

BacDive ID: 5416

Type strain: Yes

Species: Andreesenia angusta

Strain Designation: MK-1

Strain history: <- H.U. Beuscher and J.R. Andreesen, MK-1

NCBI tax ID(s): 39480 (species)

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General

@ref: 944

BacDive-ID: 5416

DSM-Number: 1989

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped, colony-forming

description: Andreesenia angusta MK-1 is an anaerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from sewage sludge.

NCBI tax id

  • NCBI tax id: 39480
  • Matching level: species

strain history

  • @ref: 944
  • history: <- H.U. Beuscher and J.R. Andreesen, MK-1

doi: 10.13145/bacdive5416.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Tissierellales
  • family: Gottschalkiaceae
  • genus: Andreesenia
  • species: Andreesenia angusta
  • full scientific name: Andreesenia angusta (Beuscher and Andreesen 1985) Poehlein et al. 2017
  • synonyms

    @refsynonym
    20215Gottschalkia angusta
    20215Eubacterium angustum

@ref: 944

domain: Bacteria

phylum: Firmicutes

class: Tissierellia

order: Clostridiales

family: Gottschalkiaceae

genus: Andreesenia

species: Andreesenia angusta

full scientific name: Andreesenia angusta (Beuscher and Andreesen 1985) Poehlein et al. 2017

strain designation: MK-1

type strain: yes

Morphology

cell morphology

  • @ref: 43456
  • gram stain: positive
  • cell length: 3-7 µm
  • cell width: 1-1.5 µm
  • cell shape: rod-shaped
  • motility: no
  • flagellum arrangement: polar

colony morphology

  • @ref: 43456
  • colony size: 0.5-1.5 mm
  • colony color: non-pigmented
  • colony shape: circular

Culture and growth conditions

culture medium

  • @ref: 944
  • name: GOTTSCHALKIA MEDIUM (DSMZ Medium 76)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/76
  • composition: Name: GOTTSCHALKIA MEDIUM (DSMZ Medium 76) Composition: Uric acid 2.0 g/l Na2CO3 1.5 g/l Yeast extract 1.0 g/l K2HPO4 0.91 g/l KOH 0.67 g/l Na-thioglycolate 0.5 g/l MgSO4 x 7 H2O 0.25 g/l CaCl2 x 2 H2O 0.015 g/l FeSO4 x 7 H2O 0.006 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l Sodium resazurin 0.0005 g/l NaOH 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l H3BO3 6e-06 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
944positivegrowth37mesophilic
43456positiveoptimum30-37mesophilic
43456positivegrowth18-45

culture pH

@refabilitytypepHPH range
43456positiveoptimum7.5-8.5alkaliphile
43456positivegrowth6.5-10

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
944anaerobe
43456obligate anaerobe

spore formation

  • @ref: 43456
  • spore formation: no

observation

  • @ref: 43456
  • observation: requires thiamine for growth but does not require selenium, tungstate or molybdate

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4345616708adenine-assimilation
4345616335adenosine-assimilation
4345617057cellobiose-assimilation
4345615824D-fructose-assimilation
4345612936D-galactose-assimilation
4345617634D-glucose-assimilation
4345616899D-mannitol-assimilation
4345616024D-mannose-assimilation
4345616988D-ribose-assimilation
4345617924D-sorbitol-assimilation
4345665327D-xylose-assimilation
4345616235guanine-assimilation
4345617368hypoxanthine-assimilation
4345630849L-arabinose-assimilation
4345617716lactose-assimilation
4345617306maltose-assimilation
4345617992sucrose-assimilation
434565291gelatin-hydrolysis
4345628017starch-hydrolysis
4345617632nitrate-reduction
4345616189sulfate-reduction
4345627226uric acid+assimilation
4345615318xanthine+assimilation

metabolite production

@refChebi-IDmetaboliteproduction
4345617968butyrateno
4345618276dihydrogenno
4345616136hydrogen sulfideno
4345630089acetateyes
4345616134ammoniayes
4345616526carbon dioxideyes
4345615740formateyes

enzymes

@refvalueactivityec
43456catalase-1.11.1.6
43456lecithinase-
43456lipase-
43456urease-3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
944sewage sludgeGöttingenGermanyDEUEurope
43456sewage plant sludgeGoettingenGermanyDEUEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Plant (Factory)
#Engineered#Waste#Sewage sludge

Safety information

risk assessment

  • @ref: 944
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 944
  • description: Eubacterium angustum strain ATCC 43737 16S ribosomal RNA gene, partial sequence
  • accession: L34612
  • length: 1464
  • database: ena
  • NCBI tax ID: 39480

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Andreesenia angusta DSM 1989GCA_001855385contigncbi39480
66792[Eubacterium] angustum strain DSM 198939480.3wgspatric39480
66792Andreesenia angusta strain DSM 198939480.4wgspatric39480
66792Andreesenia angusta MK-12775507286completeimg39480

GC content

@refGC-contentmethod
94440.3thermal denaturation, midpoint method (Tm)
4345643.7
94443.6sequence analysis

External links

@ref: 944

culture collection no.: DSM 1989, ATCC 43737

straininfo link

  • @ref: 74905
  • straininfo: 46849

literature

  • topic: Genetics
  • Pubmed-ID: 28082491
  • title: Genome Sequence of Uric Acid-Fermenting Eubacterium angustum DSM 1989T (MK-1).
  • authors: Poehlein A, Galperin MY, Andreesen JR, Daniel R
  • journal: Genome Announc
  • DOI: 10.1128/genomeA.01439-16
  • year: 2017

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
944Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1989)https://www.dsmz.de/collection/catalogue/details/culture/DSM-1989
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
43456Anja Poehlein, Natalya Yutin, Rolf Daniel, Michael Y. Galperin10.1099/ijsem.0.002008Proposal for the reclassification of obligately purine-fermenting bacteria Clostridium acidurici (Barker 1938) and Clostridium purinilyticum (Dürre et al. 1981) as Gottschalkia acidurici gen. nov. comb. nov. and Gottschalkia purinilytica comb. nov. and of Eubacterium angustum (Beuscher and Andreesen 1985) as Andreesenia angusta gen. nov. comb. nov. in the family Gottschalkiaceae fam. nov.IJSEM 67: 2711-2719 201728853681
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
74905Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46849.1StrainInfo: A central database for resolving microbial strain identifiers