Strain identifier
BacDive ID: 5416
Type strain:
Species: Andreesenia angusta
Strain Designation: MK-1
Strain history: <- H.U. Beuscher and J.R. Andreesen, MK-1
NCBI tax ID(s): 39480 (species)
General
@ref: 944
BacDive-ID: 5416
DSM-Number: 1989
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped, colony-forming
description: Andreesenia angusta MK-1 is an anaerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from sewage sludge.
NCBI tax id
- NCBI tax id: 39480
- Matching level: species
strain history
- @ref: 944
- history: <- H.U. Beuscher and J.R. Andreesen, MK-1
doi: 10.13145/bacdive5416.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Tissierellales
- family: Gottschalkiaceae
- genus: Andreesenia
- species: Andreesenia angusta
- full scientific name: Andreesenia angusta (Beuscher and Andreesen 1985) Poehlein et al. 2017
synonyms
@ref synonym 20215 Gottschalkia angusta 20215 Eubacterium angustum
@ref: 944
domain: Bacteria
phylum: Firmicutes
class: Tissierellia
order: Clostridiales
family: Gottschalkiaceae
genus: Andreesenia
species: Andreesenia angusta
full scientific name: Andreesenia angusta (Beuscher and Andreesen 1985) Poehlein et al. 2017
strain designation: MK-1
type strain: yes
Morphology
cell morphology
- @ref: 43456
- gram stain: positive
- cell length: 3-7 µm
- cell width: 1-1.5 µm
- cell shape: rod-shaped
- motility: no
- flagellum arrangement: polar
colony morphology
- @ref: 43456
- colony size: 0.5-1.5 mm
- colony color: non-pigmented
- colony shape: circular
Culture and growth conditions
culture medium
- @ref: 944
- name: GOTTSCHALKIA MEDIUM (DSMZ Medium 76)
- growth: yes
- link: https://mediadive.dsmz.de/medium/76
- composition: Name: GOTTSCHALKIA MEDIUM (DSMZ Medium 76) Composition: Uric acid 2.0 g/l Na2CO3 1.5 g/l Yeast extract 1.0 g/l K2HPO4 0.91 g/l KOH 0.67 g/l Na-thioglycolate 0.5 g/l MgSO4 x 7 H2O 0.25 g/l CaCl2 x 2 H2O 0.015 g/l FeSO4 x 7 H2O 0.006 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l Sodium resazurin 0.0005 g/l NaOH 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l H3BO3 6e-06 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
944 | positive | growth | 37 | mesophilic |
43456 | positive | optimum | 30-37 | mesophilic |
43456 | positive | growth | 18-45 |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
43456 | positive | optimum | 7.5-8.5 | alkaliphile |
43456 | positive | growth | 6.5-10 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
944 | anaerobe |
43456 | obligate anaerobe |
spore formation
- @ref: 43456
- spore formation: no
observation
- @ref: 43456
- observation: requires thiamine for growth but does not require selenium, tungstate or molybdate
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
43456 | 16708 | adenine | - | assimilation |
43456 | 16335 | adenosine | - | assimilation |
43456 | 17057 | cellobiose | - | assimilation |
43456 | 15824 | D-fructose | - | assimilation |
43456 | 12936 | D-galactose | - | assimilation |
43456 | 17634 | D-glucose | - | assimilation |
43456 | 16899 | D-mannitol | - | assimilation |
43456 | 16024 | D-mannose | - | assimilation |
43456 | 16988 | D-ribose | - | assimilation |
43456 | 17924 | D-sorbitol | - | assimilation |
43456 | 65327 | D-xylose | - | assimilation |
43456 | 16235 | guanine | - | assimilation |
43456 | 17368 | hypoxanthine | - | assimilation |
43456 | 30849 | L-arabinose | - | assimilation |
43456 | 17716 | lactose | - | assimilation |
43456 | 17306 | maltose | - | assimilation |
43456 | 17992 | sucrose | - | assimilation |
43456 | 5291 | gelatin | - | hydrolysis |
43456 | 28017 | starch | - | hydrolysis |
43456 | 17632 | nitrate | - | reduction |
43456 | 16189 | sulfate | - | reduction |
43456 | 27226 | uric acid | + | assimilation |
43456 | 15318 | xanthine | + | assimilation |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
43456 | 17968 | butyrate | no |
43456 | 18276 | dihydrogen | no |
43456 | 16136 | hydrogen sulfide | no |
43456 | 30089 | acetate | yes |
43456 | 16134 | ammonia | yes |
43456 | 16526 | carbon dioxide | yes |
43456 | 15740 | formate | yes |
enzymes
@ref | value | activity | ec |
---|---|---|---|
43456 | catalase | - | 1.11.1.6 |
43456 | lecithinase | - | |
43456 | lipase | - | |
43456 | urease | - | 3.5.1.5 |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
944 | sewage sludge | Göttingen | Germany | DEU | Europe |
43456 | sewage plant sludge | Goettingen | Germany | DEU | Europe |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Industrial | #Plant (Factory) |
#Engineered | #Waste | #Sewage sludge |
Safety information
risk assessment
- @ref: 944
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 944
- description: Eubacterium angustum strain ATCC 43737 16S ribosomal RNA gene, partial sequence
- accession: L34612
- length: 1464
- database: ena
- NCBI tax ID: 39480
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Andreesenia angusta DSM 1989 | GCA_001855385 | contig | ncbi | 39480 |
66792 | [Eubacterium] angustum strain DSM 1989 | 39480.3 | wgs | patric | 39480 |
66792 | Andreesenia angusta strain DSM 1989 | 39480.4 | wgs | patric | 39480 |
66792 | Andreesenia angusta MK-1 | 2775507286 | complete | img | 39480 |
GC content
@ref | GC-content | method |
---|---|---|
944 | 40.3 | thermal denaturation, midpoint method (Tm) |
43456 | 43.7 | |
944 | 43.6 | sequence analysis |
External links
@ref: 944
culture collection no.: DSM 1989, ATCC 43737
straininfo link
- @ref: 74905
- straininfo: 46849
literature
- topic: Genetics
- Pubmed-ID: 28082491
- title: Genome Sequence of Uric Acid-Fermenting Eubacterium angustum DSM 1989T (MK-1).
- authors: Poehlein A, Galperin MY, Andreesen JR, Daniel R
- journal: Genome Announc
- DOI: 10.1128/genomeA.01439-16
- year: 2017
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
944 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1989) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-1989 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
43456 | Anja Poehlein, Natalya Yutin, Rolf Daniel, Michael Y. Galperin | 10.1099/ijsem.0.002008 | Proposal for the reclassification of obligately purine-fermenting bacteria Clostridium acidurici (Barker 1938) and Clostridium purinilyticum (Dürre et al. 1981) as Gottschalkia acidurici gen. nov. comb. nov. and Gottschalkia purinilytica comb. nov. and of Eubacterium angustum (Beuscher and Andreesen 1985) as Andreesenia angusta gen. nov. comb. nov. in the family Gottschalkiaceae fam. nov. | IJSEM 67: 2711-2719 2017 | 28853681 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
74905 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID46849.1 | StrainInfo: A central database for resolving microbial strain identifiers |