Strain identifier

BacDive ID: 5413

Type strain: Yes

Species: Anaerofustis stercorihominis

Strain history: CIP <- 2004, CCUG <- 2003, Y. Song, VAMC, Los Angeles, USA

NCBI tax ID(s): 445971 (strain), 214853 (species)

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General

@ref: 6851

BacDive-ID: 5413

DSM-Number: 17244

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, rod-shaped

description: Anaerofustis stercorihominis DSM 17244 is an anaerobe, rod-shaped bacterium that was isolated from human feces, autistic child.

NCBI tax id

NCBI tax idMatching level
445971strain
214853species

strain history

@refhistory
6851<- CCUG <- Y.Song, VAMC
67771<- CCUG <- Y Song, WAL 14563
123311CIP <- 2004, CCUG <- 2003, Y. Song, VAMC, Los Angeles, USA

doi: 10.13145/bacdive5413.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Eubacteriaceae
  • genus: Anaerofustis
  • species: Anaerofustis stercorihominis
  • full scientific name: Anaerofustis stercorihominis Finegold et al. 2004

@ref: 6851

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Eubacteriaceae

genus: Anaerofustis

species: Anaerofustis stercorihominis

full scientific name: Anaerofustis stercorihominis Finegold et al. 2004

type strain: yes

Morphology

cell morphology

@refcell shapegram stainmotility
67771rod-shaped
67771positive
123311rod-shapednegativeno

colony morphology

  • @ref: 123311
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6851BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58)yeshttps://mediadive.dsmz.de/medium/58Name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58) Composition: Glucose 10.0 g/l Casein peptone 10.0 g/l Bacto Soytone 5.0 g/l Meat extract 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l MnSO4 x H2O 0.05 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Tween 80 Resazurin Distilled water
33967MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
123311CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6
123311CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperature
6851positivegrowth37
33967positivegrowth37
57858positivegrowth37
67771positivegrowth37

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
6851anaerobe
57858anaerobe
67771anaerobe
123311anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12331117108D-arabinose-degradation
12331115824D-fructose+degradation
12331117634D-glucose-degradation
12331165327D-xylose-degradation
12331117057cellobiose-degradation
12331117716lactose-degradation
12331117306maltose-degradation
12331117814salicin-degradation
12331117992sucrose-degradation
1233114853esculin-hydrolysis
12331117632nitrate-reduction
12331116301nitrite-reduction
12331117632nitrate+respiration
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
12331135581indoleno
6838035581indoleno

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
123311oxidase-
123311beta-galactosidase-3.2.1.23
123311gelatinase-
123311amylase-
123311DNase-
123311caseinase-3.4.21.50
123311catalase-1.11.1.6
123311tween esterase-
123311lecithinase-
123311lipase-
123311protease-
123311urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123311----------++--------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
6851-----------------------------
6851-----------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
6851human feces, autistic childLos AngelesUSAUSANorth America
57858Human feces,6-yr-old autistic boyUSAUSANorth America
67771From human feces, autistic childLos AngelesUSAUSANorth America
123311Human, FecesUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Host#Human#Child
#Host Body Product#Gastrointestinal tract#Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_4758.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_488;96_2353;97_2864;98_3565;99_4758&stattab=map
  • Last taxonomy: Anaerofustis stercorihominis subclade
  • 16S sequence: AJ518871
  • Sequence Identity:
  • Total samples: 20433
  • soil counts: 177
  • aquatic counts: 375
  • animal counts: 19796
  • plant counts: 85

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
68511Risk group (German classification)
1233111Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6851
  • description: Anaerofustis stercorihominis 16S rRNA gene, type strain wal 14563
  • accession: AJ518871
  • length: 1462
  • database: nuccore
  • NCBI tax ID: 445971

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Anaerofustis stercorihominis DSM 17244445971.6wgspatric445971
66792Anaerofustis stercorihominis DSM 17244641736193draftimg445971
67771Anaerofustis stercorihominis DSM 17244GCA_000154825scaffoldncbi445971

GC content

@refGC-content
685170
6777170.0

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesno64no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes78.478yes
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)yes87.397no
69480spore-formingspore-formingAbility to form endo- or exosporesno61.727no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no97.924yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno87.472no
69480flagellatedmotile2+Ability to perform flagellated movementno85.026no

External links

@ref: 6851

culture collection no.: DSM 17244, ATCC BAA-858, CCUG 47767, CIP 108481, WAL 14563, KCTC 15239

straininfo link

  • @ref: 74902
  • straininfo: 132772

literature

  • topic: Phylogeny
  • Pubmed-ID: 16701499
  • title: Anaerofustis stercorihominis gen. nov., sp. nov., from human feces.
  • authors: Finegold SM, Lawson PA, Vaisanen ML, Molitoris DR, Song Y, Liu C, Collins MD
  • journal: Anaerobe
  • DOI: 10.1016/j.anaerobe.2003.10.002
  • year: 2004

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6851Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17244)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17244
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33967Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6108
57858Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 47767)https://www.ccug.se/strain?id=47767
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
74902Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID132772.1StrainInfo: A central database for resolving microbial strain identifiers
123311Curators of the CIPCollection of Institut Pasteur (CIP 108481)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108481