Strain identifier

BacDive ID: 5413

Type strain: Yes

Species: Anaerofustis stercorihominis

Strain history: CIP <- 2004, CCUG <- 2003, Y. Song, VAMC, Los Angeles, USA

NCBI tax ID(s): 445971 (strain), 214853 (species)

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General

@ref: 6851

BacDive-ID: 5413

DSM-Number: 17244

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, rod-shaped

description: Anaerofustis stercorihominis DSM 17244 is an anaerobe, mesophilic, rod-shaped bacterium that was isolated from human feces, autistic child.

NCBI tax id

NCBI tax idMatching level
214853species
445971strain

strain history

@refhistory
6851<- CCUG <- Y.Song, VAMC
67771<- CCUG <- Y Song, WAL 14563
123311CIP <- 2004, CCUG <- 2003, Y. Song, VAMC, Los Angeles, USA

doi: 10.13145/bacdive5413.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Eubacteriaceae
  • genus: Anaerofustis
  • species: Anaerofustis stercorihominis
  • full scientific name: Anaerofustis stercorihominis Finegold et al. 2004

@ref: 6851

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Eubacteriaceae

genus: Anaerofustis

species: Anaerofustis stercorihominis

full scientific name: Anaerofustis stercorihominis Finegold et al. 2004

type strain: yes

Morphology

cell morphology

@refcell shapegram stainconfidencemotility
67771rod-shaped
67771positive
69480positive100
123311rod-shapednegativeno

colony morphology

  • @ref: 123311
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6851BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58)yeshttps://mediadive.dsmz.de/medium/58Name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58) Composition: Glucose 10.0 g/l Casein peptone 10.0 g/l Bacto Soytone 5.0 g/l Meat extract 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l MnSO4 x H2O 0.05 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Tween 80 Resazurin Distilled water
33967MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
123311CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6
123311CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperaturerange
6851positivegrowth37mesophilic
33967positivegrowth37mesophilic
57858positivegrowth37mesophilic
67771positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
6851anaerobe
57858anaerobe
67771anaerobe
69480anaerobe99.999
123311anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 98.808

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12331117108D-arabinose-degradation
12331115824D-fructose+degradation
12331117634D-glucose-degradation
12331165327D-xylose-degradation
12331117057cellobiose-degradation
12331117716lactose-degradation
12331117306maltose-degradation
12331117814salicin-degradation
12331117992sucrose-degradation
1233114853esculin-hydrolysis
12331117632nitrate-reduction
12331116301nitrite-reduction
12331117632nitrate+respiration
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
12331135581indoleno
6838035581indoleno

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
123311oxidase-
123311beta-galactosidase-3.2.1.23
123311gelatinase-
123311amylase-
123311DNase-
123311caseinase-3.4.21.50
123311catalase-1.11.1.6
123311tween esterase-
123311lecithinase-
123311lipase-
123311protease-
123311urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123311----------++--------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
6851-----------------------------
6851-----------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
6851human feces, autistic childLos AngelesUSAUSANorth America
57858Human feces,6-yr-old autistic boyUSAUSANorth America
67771From human feces, autistic childLos AngelesUSAUSANorth America
123311Human, FecesUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Host#Human#Child
#Host Body Product#Gastrointestinal tract#Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_4758.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_488;96_2353;97_2864;98_3565;99_4758&stattab=map
  • Last taxonomy: Anaerofustis stercorihominis subclade
  • 16S sequence: AJ518871
  • Sequence Identity:
  • Total samples: 20433
  • soil counts: 177
  • aquatic counts: 375
  • animal counts: 19796
  • plant counts: 85

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
68511Risk group (German classification)
1233111Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6851
  • description: Anaerofustis stercorihominis 16S rRNA gene, type strain wal 14563
  • accession: AJ518871
  • length: 1462
  • database: ena
  • NCBI tax ID: 445971

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Anaerofustis stercorihominis DSM 17244445971.6wgspatric445971
66792Anaerofustis stercorihominis DSM 17244641736193draftimg445971
67771Anaerofustis stercorihominis DSM 17244GCA_000154825scaffoldncbi445971

GC content

@refGC-content
685170
6777170.0

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno64no
gram-positiveyes91.407yes
anaerobicyes98.948yes
halophileno65.619no
spore-formingno67.246no
glucose-utilyes84.58no
aerobicno98.61yes
flagellatedno96.414no
thermophileno96.095yes
motileno90.335no
glucose-fermentyes55.885no

External links

@ref: 6851

culture collection no.: DSM 17244, ATCC BAA 858, CCUG 47767, CIP 108481, WAL 14563, KCTC 15239

straininfo link

  • @ref: 74902
  • straininfo: 132772

literature

  • topic: Phylogeny
  • Pubmed-ID: 16701499
  • title: Anaerofustis stercorihominis gen. nov., sp. nov., from human feces.
  • authors: Finegold SM, Lawson PA, Vaisanen ML, Molitoris DR, Song Y, Liu C, Collins MD
  • journal: Anaerobe
  • DOI: 10.1016/j.anaerobe.2003.10.002
  • year: 2004

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6851Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17244)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17244
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33967Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6108
57858Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 47767)https://www.ccug.se/strain?id=47767
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
74902Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID132772.1StrainInfo: A central database for resolving microbial strain identifiers
123311Curators of the CIPCollection of Institut Pasteur (CIP 108481)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108481